Toxoplasmosis in adult patients with haematologic malignancy: Seroprevalence of anti-Toxoplasma antibodies and molecular diagnosis

Shawa, Taha Mohammed; El-Taweel, Hend A; El-Gammal, Maha; Khalil, Safia; Ibrahim, Heba

doi:10.21608/puj.2023.203903.1209

Cited by 1 publication

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“…Nearly, 39.3% of the population of Iran and 35.1-41% of people in the Northwest Iran are infected with toxoplasmosis [ 2 , 3 ]. Latent toxoplasmosis has long been considered asymptomatic in immunocompetent people, but it can result in serious or life-threatening disease in patients with haematologic malignancy, immunocompromised cancer patients, organ transplants and pregnant women [ 1 , 4 – 6 ]. Toxoplasmosis is also associated with various autoimmune [ 7 ] and inflammatory diseases [ 8 ] as well as some cancers [ 9 , 10 ].…”

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confidence: 99%

Genetic diversity and seroprevalence of Toxoplasma gondii in COVID‑19 patients; a first case-control study in Iran

Hasanzadeh,

Ahmadpour,

Mahami-Oskouei

et al. 2024

BMC Infect Dis

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Background Toxoplasmosis is a serious or life-threatening disease in immunosuppressed patients and pregnant women. This study examined the likely association between Toxoplasma gondii infection and COVID-19 patients with moderate illness. Methods Seventy blood samples were collected from patients at the Health Reference Laboratory of Tabriz, Northwest Iran from April 2021 to September 2021. In addition, 70 healthy subjects of the same age (37 ± 15 years) and sex distribution were ethnically matched. Sera samples were examined for the detection of anti-Toxoplasma antibodies using ELISA. Nested-PCR targets were amplified based on the B1 and GRA6 genes. GRA6 amplicons were subjected to sequencing and phylogenetic analysis. Results The seroprevalence of toxoplasmosis based on IgG titer was 35.7% in the COVID‑19 patients and 27.1% in the control group, representing not to be associated with the Toxoplasma seropositivity in COVID‑19 patients (P = 0.18) compared to healthy subjects. Anti-T. gondii IgM was not found in any of the patients and healthy individuals. According to PCR amplification of the B1 and GRA6 genes, the frequency of T. gondii in COVID-19 patients was 14.2% (10/70). However, no T. gondii infection was detected in the healthy group. The CD4+T cell count was relatively lower in toxoplasmosis-infected patients (430–450 cells/mm3) than in control group (500–1500 cells/mm3). High genetic diversity (Hd: 0.710) of the type I strain of T. gondii was characterized in the patients. Present results showed that consumption of raw vegetables and close contact with stray cats can increase the transmission of T. gondii to COVID-19 patients (P < 0.01). Conclusions The current study revealed that T. gondii type I infection is unequivocally circulating among the COVID-19 patients in Tabriz; However, no significant association was observed between the occurrence of Toxoplasma and the severity of COVID-19. To make more accurate health decisions, multicenter investigations with a larger sample size of different ethnic groups of the Iranian population are needed.

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