Toxoplasmose: epidemiologia e importância da doença na saúde animal

Vidotto, Odilon

doi:10.5433/1679-0359.1992v13n1p69

Cited by 16 publications

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“…Many serologic tests have been performed by several investigators in an attempt to demonstrate which is the most effective in the diagnosis of toxoplasmosis (ISHIZUKA;MIGUEL;BROGLIATO, 1974;CALAMEL;LAMBERT, 1985;KRAMER;WEISBRODE 1985;VIDOTTO, 1992).…”

Section: Introductionmentioning

confidence: 99%

Detection of anti-Toxoplasma gondii antibodies in experimentally and naturally infected non-human primates by Indirect Fluorescence Assay (IFA) and indirect ELISA

Bouer

Werther

Machado

et al. 2010

RBPV

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Section: Introductionmentioning

confidence: 99%

Detection of anti-Toxoplasma gondii antibodies in experimentally and naturally infected non-human primates by Indirect Fluorescence Assay (IFA) and indirect ELISA

Bouer

Werther

Machado

et al. 2010

RBPV

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“…In North Paraná herds, Brazil, Garcia et al (1999) detected 24% of seropositive pigs. Many of the variations in the antibody rates against T. gondii result from management conditions and type of breeding, as well as from the different serological tests used (Vidotto 1992).…”

Section: Introduction Introduction Introduction Introduction Introducmentioning

confidence: 99%

Evaluation of the indirect fluorescent antibody test and modified agglutination test for detection of antibodies against Toxoplasma gondii in experimentally infected pigs

et al. 2004

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“…Many serologic tests have been performed by several investigators in an attempt to demonstrate which is the most effective in the diagnosis of toxoplasmosis (ISHIZUKA; MIGUEL; BROGLIATO, 1974;CALAMEL;LAMBERT, 1985;KRAMER;WEISBRODE 1985;VIDOTTO, 1992).…”

Section: Introductionmentioning

confidence: 99%

Detection of anti-Toxoplasma gondii antibodies in experimentally and naturally infected non-human primates by Indirect Fluorescence Assay (IFA) and indirect ELISA

Bouer¹,

Werther²,

Machado³

et al. 2010

Rev. Bras. Parasitol. Vet. (Online)

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The Indirect Fluorescence Assay (IFA) and the indirect ELISA were comparatively used to detect IgG and IgM antibodies for Toxoplasma gondii in experimentally and naturally infected primates. In the experimentally infected group, antibodies of diagnostic value were detected at day 9 post-infection (PI) with the IFA (IgG and IgM) and with IgG-ELISA. IgM-ELISA detected antibodies for T. gondii starting at day 3 PI until the end of the experiment (102 days PI). Of the 209 naturally infected sera tested, from many zoos of State of Sao Paulo, 64.59 and 67.94% were positive in the IgG-IFA test and IgG-ELISA respectively. IgM-ELISA test detected seropositivity in 52.63% of the sera although IgM-IFA test detected it in only in 0.96% of the samples. The differential toxoplasmosis diagnosis was accomplished with Neospora caninum by IFA, observing 61 (29.2%) seropositive animals for this parasite and 149 (70.8%) negative. Sixty animals were positive for both T. gondii and N. caninum. Pneumonia, splenomegaly, and intestinal ulcers were macroscopically observed. Unremarkable interstitial pneumonia, enteritis, colitis, splenitis, and glomerulitis were microscopically observed. The immunohistochemical stain could not detect the presence of T. gondii in the tissues of the animals infected experimentally.Keywords: Toxoplasma gondii, toxoplasmosis, primates, Indirect Fluorescence Assay, ELISA. ResumoDetectou-se anticorpos das classes IgG e IgM anti-Toxoplasma gondii em primatas experimentalmente e naturalmente infectados, utilizando-se como técnicas comparativas a RIFI e o ELISA-teste. No grupo dos primatas experimentalmente infectados, anticorpos de valor diagnóstico foram detectados a partir do 9º dia de infecção tanto na RIFI (IgG e IgM) como no ELISA-IgG. O ELISA IgM detectou anticorpos a partir do 3º dia de infecção até o final do experimento (102 dias pós-infecção). Dos 209 soros dos primatas naturalmente infectados, de diversos zoológicos do Estado de São Paulo, 64,59 e 67,94% mostraram-se positivos na RIFI-IgG e no ELISA-IgG, respectivamente. O ELISA-IgM detectou soropositividade em 52,63% dos soros ao passo que a RIFI-IgM detectou apenas 0,96%. Foi realizado também diagnóstico diferencial para Neospora caninum, através da RIFI, observando-se 61 (29,2%) animais soropositivos para este parasita e 149 (70,8%) animais negativos. Sessenta animais foram positivos para T. gondii e N. caninum. Pneumonia, esplenomegalia e úlceras intestinais foram observadas macroscopicamente. Pneumonia intersticial, enterite, colite, esplenite e glomerulite foram os achados microscópicos. A imunoistoquímica não revelou a presença do T. gondii nos tecidos dos animais experimentalmente infectados.

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Toxoplasmose: epidemiologia e importância da doença na saúde animal

Cited by 16 publications

References 0 publications

Detection of anti-Toxoplasma gondii antibodies in experimentally and naturally infected non-human primates by Indirect Fluorescence Assay (IFA) and indirect ELISA

Detection of anti-Toxoplasma gondii antibodies in experimentally and naturally infected non-human primates by Indirect Fluorescence Assay (IFA) and indirect ELISA

Evaluation of the indirect fluorescent antibody test and modified agglutination test for detection of antibodies against Toxoplasma gondii in experimentally infected pigs

Detection of anti-Toxoplasma gondii antibodies in experimentally and naturally infected non-human primates by Indirect Fluorescence Assay (IFA) and indirect ELISA

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