“…Out of 57 studies describing the evaluation of diagnostic performance of different ELISAs for the detection of T. gondii antibodies, the majority originated from Europe ( n = 21), followed by Asia ( n = 16), South America ( n = 9), North America ( n = 6), Africa ( n = 3) and Australia ( n = 2) ( Figure 2 ). Out of the 20 animal species covered, most of the studies (91%) focused on domesticated animals [ 33 , 37 , 46 , 47 , 48 , 49 , 50 ], but 7% were on zoo and wild animals [ 28 , 44 , 51 , 52 ], and one study targeted both domestic and wild animals [ 43 ]. Serum was the most widely used (89% of studies) sample for the detection of T. gondii -specific antibodies in animals, followed by meat juice/tissue fluid (7%) [ 37 , 45 , 53 , 54 ] and milk (4%) [ 55 , 56 ].…”