Toxoplasma gondii Isolates of Free-Ranging Chickens From Rio de Janeiro, Brazil: Mouse Mortality, Genotype, and Oocyst Shedding by Cats

Dubey, J. P.; Graham, Douglas H.; Silva, Daniele Seipel da; Lehmann, Tovi; Bahia-Oliveira, Lilian M. G.

doi:10.1645/ge-60r

Cited by 99 publications

(47 citation statements)

References 33 publications

(19 reference statements)

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“…Three virulent strains (CH4, CH5 and CH10) in mice were type I and one (CH11) was type I or II. Our results are in agreement with those by Dubey et al (2002Dubey et al ( , 2003a, who observed a predominance of T. gondii isolates as being type I in chickens obtained in the States of São Paulo and Rio de Janeiro. On the other hand, other reports have revealed that T. gondii isolates from chickens obtained in Argentina (Dubey et al, 2003c), Egypt (Dubey et al, 2003d) and the US (Dubey et al, 2003e) were mostly type III, suggesting a different epidemiological prevalence of T. gondii genotypes in Brazil, as compared to other countries.…”

Section: Discussionsupporting

confidence: 93%

“…Howe et al (1997) developed a typing system for T. gondii strains based on analysis of restriction fragments of the gene SAG2, amplified by nested-PCR, which allows a differentiation among strain genotypes of the three clonal lineages, previously described by Howe & Sibley (1995). In Brazil, this method was used for analyzing T. gondii isolates from São Paulo (Dubey et al, 2002), Rio de Janeiro (Dubey et al, 2003a) and Paraná (Dubey et al, 2003b), which showed to be predominantly type I. Ferreira et al (2006), using a multilocus PCR-RFLP, demonstrated that Brazilian strains present recombinant genotypes with typical alleles of the strains types I, II, and III in the majority of the loci.…”

Section: Introductionmentioning

confidence: 99%

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Characterization ofToxoplasma gondiifrom domestic animals from Minas Gerais, Brazil

Brandão

Ferreira²,

Melo³

et al. 2006

Parasite

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Summary :In an attempt to isolate and characterize Toxoplasma gondii from the State of Minas Gerais, Brazil, musculature samples from 72 pigs, 25 dogs, 28 free-range chickens and 50 chickens produced in industrialized farms were collected. Antibodies to T. gondii have not been detected in pigs, but were found in nine (40.9 %) out of 22 dogs, and in 15 (53.6 %) of 28 free range chickens. T. gondii was not isolated from pigs and industrialized chickens, but from eight dogs and 11 free range chickens. In order to determine T. gondii virulence, female BALB/c mice were inoculated with 10 3 , 10 2 , 10 1 and 10 0 tachyzoites of the 19 isolates. The strains RH (virulent) and ME49 (non-virulent) were used as references. Isolates were divided into three groups according to the virulence phenotype: five isolates were classified into virulent in mice, one into non-virulent and 13 into intermediate virulent. Nested-PCR of T. gondii SAG2 locus amplified DNA from 21 out of 22 DNA samples directly extracted from heart of free range chickens. These samples were genotyped through a PCR-RFLP assay. Seventeen (80.9 %) were classified into type I; one (4.8 %) into type III and three (14.3 %) into type I or II. Résumé

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Characterization ofToxoplasma gondiifrom domestic animals from Minas Gerais, Brazil

Brandão

Ferreira²,

Melo³

et al. 2006

Parasite

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“…However, such meats are under sanitary control, and hence, neosporosis and toxoplasmosis from these sources were not investigated in the present survey. The seroprevalence of T. gondii among chickens in Brazil ranges from 39% to 66% (DA SILVA et al, 2003;DUBEY et al, 2003;DUBEY et al, 2006;DE OLIVEIRA et al, 2009); and among cattle, from 1% (GONDIM et al, 1999) to 71% (SANTOS et al, 2009). On the other hand, the seroprevalence of N. caninum among cattle in Brazil ranges from 14.3% (GUIMARÃES et al, 2004) to 91.2% (GUEDES et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Occurrence of anti-Toxoplasma gondii and anti-Neospora caninum antibodies in cats with outdoor access in São Luís, Maranhão, Brazil

Braga

André

Jusi

et al. 2012

Rev. Bras. Parasitol. Vet.

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Occurrence of anti-Toxoplasma gondii and anti-Neospora caninum antibodies in cats with outdoor access in São Luís, Maranhão, Brazil AbstractThe present study aimed to investigate the frequency of anti-Toxoplasma gondii and anti-Neospora caninum antibodies in cats with outdoor access in São Luís, Maranhão, Brazil. The presence of IgG anti-T. gondii and anti-N. caninum antibodies was tested using the Indirect Immunofluorescent Antibody Test (IFAT). IgG anti-T. gondii and anti-N. caninum antibodies were detected in 101 (50.5%) and 54 (27%) sampled cats, respectively. The titers of anti-T. gondii antibodies ranged from 40 (cut-off) to 2560. On the other hand, the titers of anti-N. caninum antibodies ranged from 25 (cut-off) to 400. Twenty-seven cats (13.5%) were shown to be seropositive for both parasites. Seventy-four cats (34%) were seropositive only for T. gondii. Twenty-two cats (11%) were seropositive only for N. caninum. The present study showed that cats with outdoor access in São Luís, Maranhão, are exposed to T. gondii and N. caninum.Keywords: Toxoplasma gondii, Neospora caninum, cats, serology. ResumoO presente estudo objetivou verificar a frequência de anticorpos anti-Toxoplasma gondii e anti-Neospora caninum em gatos com acesso à rua em São Luís, Maranhão, Brasil. A presença de anticorpos IgG anti-T. gondii e anti-N. caninum foi verificado pela Reação de Imunofluorescência Indireta (RIFI). Anticorpos IgG anti-T. gondii e anti-N. caninum foram detectados em 101 (50,5%) e 54 (27%) gatos amostrados, respectivamente. Os títulos de anticorpos anti-T. gondii variaram de 40 (ponto de corte) a 2560. Por outro lado, anticorpos anti-N. caninum variaram de 25 (ponto de corte) a 400. Vinte e sete gatos (13,5%) mostraram-se soropositivos para ambos os parasitas. Setenta e quatro gatos (34%) foram soropositivos somente para T. gondii. Vinte e dois gatos (11%) foram soropositivos somente para N. caninum. O presente estudo demonstrou que gatos com acesso à rua em São Luís, Maranhão, são expostos ao T. gondii e N. caninum.

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“…Estudos de prevalência do T. gondii em fazendas de ovinos com manejo intensivo no Reino Unido ressaltam a importância da transmissão congênita e relatam que o risco de outras vias de infecção é reduzido, podendo ser a transmissão congênita suficiente para explicar a manutenção do agente nessas populações (ENGELAND et al, 1996;DUNCANSON et al, 2001 Brasil em outras espécies animais como em cães , galinhas DUBEY et al, 2003d;;DUBEY et al, 2003e), gatos (DUBEY et al, 2003;PENA et al, 2006), suínos (SANTOS et al, 2005, SOUSA et al, 2006 MONDRAGON et al,1998;FUENTES et al, 2001;ASPINALL et al, 2003). Os isolados tipo I têm se apresentando virulentos a camundongos com alta parasitemia quando comparados com os outros genótipos e com uma possível correlação com infecção congênita em humanos.…”

Section: -Discussãounclassified

“…Os isolados tipo I têm se apresentando virulentos a camundongos com alta parasitemia quando comparados com os outros genótipos e com uma possível correlação com infecção congênita em humanos. Isolados tipo II foram os mais prevalentes tanto em humanos como em animais em várias partes do mundo, havendo uma possível correlação com reativação da toxoplasmose em indivíduos imunocomprometidos (HOWE et al, 1997;MONDRAGON et al,1998;FUENTES et al, 2001 , DUBEY et al, 2003d, DUBEY et al, 2003e, DUBEY et al, 2006FERREIRA et al, 2006, DUBEY et al 2007). Outras espécies animais estudadas no Brasil, também apresentaram perfil semelhante , SANTOS et al, 2005, YAI, et al, 2006 Utilizando métodos moleculares com maior resolução, é observada a ocorrência de padrões recombinantes ou atípicos entre as amostras brasileiras (CAVALCANTE et al, 2006b;DUBEY et al 2007;DUBEY et al, 2007a).…”

Section: -Discussãounclassified

Isolamento e caracterização biológica e genotipica de Toxoplasma gondii de ovinos e caprinos

Ragozo¹

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2007Autorizo a reprodução parcial ou total desta obra, para fins acadêmicos, desde que citada a fonte. Os três meios primários para a difusão deste protozoário são a transmissão transplacentária, a ingestão de tecidos animais contendo cistos infectantes e a ingestão de alimentos e água contaminados com fezes de gatos contendo oocistos esporulados. Atualmente não há métodos que determinem se um DADOS INTERNACIONAIS DE CATALOGAÇÃO-NA-PUBLICAÇÃO (Biblioteca VirginieBuff

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Toxoplasma gondii Isolates of Free-Ranging Chickens From Rio de Janeiro, Brazil: Mouse Mortality, Genotype, and Oocyst Shedding by Cats

Cited by 99 publications

References 33 publications

Characterization ofToxoplasma gondiifrom domestic animals from Minas Gerais, Brazil

Characterization ofToxoplasma gondiifrom domestic animals from Minas Gerais, Brazil

Occurrence of anti-Toxoplasma gondii and anti-Neospora caninum antibodies in cats with outdoor access in São Luís, Maranhão, Brazil

Isolamento e caracterização biológica e genotipica de Toxoplasma gondii de ovinos e caprinos

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