Toxoplasma gondii: A simple high-throughput assay for drug screening in vitro

Jin, Chun Mei; Kaewintajuk, Kusuma; Jiang, Jing; Jeong, Woo Jin; Kamata, Masaki; Kim, Hye Sook; Wataya, Yusuke; Park, Hyun

doi:10.1016/j.exppara.2008.10.006

Cited by 30 publications

(27 citation statements)

References 24 publications

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“…Drugs were tested for anti-Toxoplasma activity in vitro by targeting the parasite tachyzoite form as previously described (33), with some modifications. HFFs were harvested during exponential growth (day 2) and cultured in 96-well plates (100-l suspension consisting of 6 ϫ 10 4 cells/ml; Costar, USA).…”

Section: Methodsmentioning

confidence: 99%

Repurposing the Open Access Malaria Box To Discover Potent Inhibitors of Toxoplasma gondii and Entamoeba histolytica

Boyom

Fokou

Tchokouaha

et al. 2014

Antimicrob Agents Chemother

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Section: Methodsmentioning

confidence: 99%

Repurposing the Open Access Malaria Box To Discover Potent Inhibitors of Toxoplasma gondii and Entamoeba histolytica

Boyom

Fokou

Tchokouaha

et al. 2014

Antimicrob Agents Chemother

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“…The first two stages are followed by the multistep identification and validation of drug targets, which in the case of T. gondii as an intracellular parasite should be extended to the host cells [20][21][22].…”

Section: Preliminary Testing Of Plant-derived and New Synthetic Compomentioning

confidence: 99%

Human toxoplasmosis–Searching for novel chemotherapeutics

Antczak

Dzitko

Długońska

2016

Biomedicine & Pharmacotherapy

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“…Over the past many years, the conventional Trypan Blue has been used for assessing cell viability of various parasites and cells as well (Gold 1997;Wiehart et al 2006;Jin et al 2009;Tan et al 2010). MF has been used widely on malaria (Field 1940;Fenton and Innes 1945;Reilly et al 1997), filaria (Sivanandam and Mak 1975), Leishmania (Chunge et al 1989), Acanthamoeba spp.…”

Section: Discussionmentioning

confidence: 99%

Modified Field stain - rapid viability test for Trichomonas vaginalis

Afzan

Sivanandam

2011

Journal of Applied Microbiology

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Aims: We previously reported that Modified Field Stain (MF) can be used as a rapid stain for diagnosis. In the present study we extend the observation to include the stain as an alternative method to assess viability of the cells. Methods and Results: Six isolates of Trichomonas vaginalis were used to assess the utility of the Modified Field stain as a rapid viability test for T. vaginalis and to compare with 0AE4% Trypan Blue dye exclusion test in three conditions; normal in vitro culture growth using Hollander medium, lysed in distilled water and treated with metronidazole. MF stain showed similar growth profile pattern as Trypan Blue dye exclusion for identifying viable cells of T. vaginalis. Although, Trypan Blue dye exclusion test is ready made, rapid and widely used in laboratory as reliable viability assay, however, the limitation using Trypan Blue is the dye was unable to show internal morphological changes during the parasite's transition from being viable to non-viable. On day 3 where cultures peaked the correlation factor of both assays done to assess the viability of parasites harvested from the controls, metronidazole and distilled water treated parasites were more than 0AE9 respectively. Conclusions: This confirms that MF staining does not only record permanently the morphological changes and retain internal structural details but also provides a reliable and rapid viability assay for the parasites. Significance and Impact of the Study: Therefore, in our study, Modified Field's stain may offer the researchers and laboratory technologists the opportunity to get the result on the same day and the most important thing is the ability to differentiate between viable and non-viable of T. vaginalis under three different conditions (normal culture, drug and distilled water condition). Modified Field's staining method enhanced the morphological identification of T. vaginalis compared to Trypan Blue dye exclusion.

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Toxoplasma gondii: A simple high-throughput assay for drug screening in vitro

Cited by 30 publications

References 24 publications

Repurposing the Open Access Malaria Box To Discover Potent Inhibitors of Toxoplasma gondii and Entamoeba histolytica

Repurposing the Open Access Malaria Box To Discover Potent Inhibitors of Toxoplasma gondii and Entamoeba histolytica

Human toxoplasmosis–Searching for novel chemotherapeutics

Modified Field stain - rapid viability test for Trichomonas vaginalis

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