Toxocara vitulorum infection in German beef cattle

Venjakob, P.L.; Thiele, G.; Clausen, Peter‐Henning; Nijhof, Ard M.

doi:10.1007/s00436-017-5393-2

Cited by 20 publications

(14 citation statements)

References 15 publications

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“…T. vitulorum larvae are passed in great numbers in the buffalo cows colostrum 2-5 days post-calving, worms are matured in the intestine of the calves by 10 days of age and eggs are passed in feces approximately at the 3rd week of life and then adult worms are expelled from the intestine by the 5th month of age 22,23 . In Europe, this nematode parasite was reported in the latest years at the farm level in cattle [24][25][26][27][28][29] and bisons 30 . In western Romania, the parasite was detected in 0.3% of 303 fceal samples from cattle 31 .…”

Section: Discussionmentioning

confidence: 99%

Intestinal Parasites of Buffalo Calves from Romania: Molecular Characterization of Cryptoporidium spp. and Giardia Duodenalis, and the first Report of Eimeria Bareillyi

Bărburaș

Cozma

Ionică

et al. 2021

Preprint

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Buffaloes represent an important economic resource for several regions of the world including Romania; however, no reports on parasitic infections in buffaloes from Romania are available. In the present study, we examined for the gastrointestinal parasites 104 fecal samples bimonthly collected from 38 buffalo calves (2–11 weeks old) from household rearing systems in Romania. All samples were tested using the saturated salt flotation, McMaster and modified Ziehl-Nielsen staining methods. PCR coupled with isolates sequencing methods were used to identify the Giardia duodenalis assemblages and Cryptosporidium species. Overall, 33 out of 38 examined buffalo calves were infected with different gastrointestinal parasites; 16 had single infections and 17 had mixed infections with 2 or 3 parasites. Eimeria species (32/38; 84.2%) was the most prevalent parasite; 8 species were identified according to the oocyst morphology including the pathogenic E. bareillyi which detected for the first time in buffaloes from Romania. Toxocara vitulorum (11/38; 36.8%) and Strongyloides papillosus (6/38; 15.8%) were also detected. Cryptosporidium spp. were found in 4 (10.5%) buffalo calves; 2 of them were molecularly identified as C. ryanae and another one was clustered in the same clade with C. ryanae, C. bovis, and C. xiaoi. Giardia duodenalis assemblage E was also molecularly detected in a single (2.6%) buffalo calf. The presence of other buffaloes in the same barn was identified as a risk factor for infection with T. vitulorum. Our results indicate extensive parasitic infections in buffalo calves from Northwestern Romania and underline the necessity of prophylactic treatments for T. vitulorum and E. bareillyi.

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Section: Discussionmentioning

confidence: 99%

Intestinal Parasites of Buffalo Calves from Romania: Molecular Characterization of Cryptoporidium spp. and Giardia Duodenalis, and the first Report of Eimeria Bareillyi

Bărburaș

Cozma

Ionică

et al. 2021

Preprint

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“…To do so, calves were administered an anthelmintic dosage of piperazine citrate followed by a dosage of magnesium sulphate as a purgative. Six hours later, worms expelled in feces were collected into 0.9% physiological saline in a glass container and were immediately transferred to the laboratory at room temperature [33]. Adult females were differentiated from males under the dissecting microscope (Optika, Italy) based on the differential morphological features [34].…”

Section: Collection Of Adult T Vitulorum Wormsmentioning

confidence: 99%

Molecular and microscopic detection of natural and experimental infections of Toxocara vitulorum in bovine milk

Dewair

Bessat

2020

PLoS ONE

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Toxocara vitulorum is an Ascarid nematode infecting the small intestine of buffalo and cattle particularly neonate calves, with the postnatal route through milk is the main infection source. However, little is known about shedding rates and the optimum detection methods of T. vitulorum larvae in the milk of the infected bovine hosts. In this study, we aimed to evaluate the use of two methods, microscopy and PCR, and their detection limits both under the experimental and natural infection situations. In doing this, T. vitulorum eggs extracted from naturally occurring adult female worms were successfully subjected to experimental embryonation, and larvae were implemented in experimental infection of milk in ascending infection doses of 0, 1, 5, 10, 20, 50 larvae/2-ml milk samples. With the except of negative control, microscopy-based examination detected larvae in all samples, albeit with means, ranges, and the total number of larvae were detected in exponential rates relative to larvae densities in milk samples. PCR technique corresponded well to microscopy in detecting genomic DNA of T. vitulorum larvae in all milk samples down to a single larva/sample. On the other hand, and by applying the same methodology approach on 50 naturally-occurring bovine colostrum/milk samples, 13 (26%) and 20 (40%) samples were tested positive for T. vitulorum infection by microscopy and the PCR-based detection, respectively. Of these, 11 out of 26 buffalo samples (42.30%) and 2 out of 24 cow samples (8.33%) were tested positive by microscopy, while 16 (61.54%) and 3 (12.50%) of buffalo and cow samples were tested positive by PCR, respectively. By applying the Agreement Coefficient, substantial agreement (0.77) between molecular and microscopy detection was detected from all tested samples. In conclusion, larvae of T. vitulorum were unequivocally detected by microscopy and molecular methods in milk samples both under the experimental and natural field situations. Nevertheless, slightly higher rates by PCR than microscopy were obtained when detecting naturally-infected milk samples. To the best of our knowledge, this is the first in situ detection of larvae of T. vitulorum in the milk of the naturally infected animals.

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“…Inzwischen hat diese Parasitose Europa erreicht. Es gibt Berichte aus Großbritannien (56), Schottland (84), den Niederlanden (11) und neuestens auch aus Deutschland (95).…”

Section: Toxocarose Der Rinderunclassified

“…3 Wochen post partum. Wie ein Fallbericht aus Ostdeutschland dokumentiert, können auch adulte Würmer im Kot gefunden werden (95). Morphologische und molekularbiologische Untersuchungsmethoden stehen zur Verfügung.…”

Section: Diagnoseunclassified

Vernachlässigte, neue und wiederkehrende Parasitosen in Deutschland – bedeutend für die Großtierpraxis?

Klaus¹,

Conraths²,

Schares³

et al. 2017

Tierarztl Prax Ausg G

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Für Studium und Praxis SchlüsselwörterGiardia spp., Besnoitia besnoiti, Fasciola hepatica, Toxocara vitulorum, Hypoderma spp., Culicoides spp. ZusammenfassungIn der tierärztlichen Praxis spielen Parasitosen und deren Bekämpfung nach wie vor eine wesentliche Rolle. Die vergangenen Jahre haben Veränderungen der Rahmenbedingungen mit sich gebracht, unter denen sich auch Parasitosen entwickeln und verbreiten können. Hier sind vor allem die fortschreitende Globalisierung mit weltweit zunehmendem Tier-und Warenverkehr sowie die längerfristig wirkenden klimatischen Veränderungen zu nennen. An je zwei Beispielen aus der Protozoologie, der Helminthologie und der Entomologie wird der aktuelle Kenntnisstand dargestellt, wobei neu auftretende und wiederkehrende, ebenso wie längst bekannte, aber wenig beachtete Parasitosen Berück sichtigung finden. KeywordsGiardia spp., Besnoitia besnoiti, Fasciola hepatica, Toxocara vitulorum, Hypoderma spp., Culicoides spp. SummaryIn veterinary medicine, parasite-induced diseases and their control continue to play an important role. Recent years have brought changes in the framework conditions that also favor the development and spread of parasitic diseases. Globalization in particular, accompanied by increasing global movement of animals and goods, as well as climatic changes with long-term effects should be included in this context. We present the current state of knowledge, providing two examples each of protozoal, helminthic and entomologic diseases. Emerging and re-emerging as well as neglected diseases, which have been long known but have received little attention, are con sidered. Korrespondenzadresse Dr. habil.

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Toxocara vitulorum infection in German beef cattle

Cited by 20 publications

References 15 publications

Intestinal Parasites of Buffalo Calves from Romania: Molecular Characterization of Cryptoporidium spp. and Giardia Duodenalis, and the first Report of Eimeria Bareillyi

Intestinal Parasites of Buffalo Calves from Romania: Molecular Characterization of Cryptoporidium spp. and Giardia Duodenalis, and the first Report of Eimeria Bareillyi

Molecular and microscopic detection of natural and experimental infections of Toxocara vitulorum in bovine milk

Vernachlässigte, neue und wiederkehrende Parasitosen in Deutschland – bedeutend für die Großtierpraxis?

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