Toxocara vitulorum ile doğal enfekte buzağılarda serum haptoglobin düzeyi, oksidatif belirteçler ve bazı biyokimyasal parametrelerin düzeyinin belirlenmesi

Abstract

Özet: Bu çalışmanın amacı Toxocara vitulorum ile doğal enfekte buzağılarda serum haptoglobin düzeyi, oksidatif belirteçler ve bazı biyokimyasal parametrelerin düzeylerinin belirlenmesi ve diyagnostik önemlerinin araştırılmasıdır. Bu amaçla, kontrol grubu olarak 10 adet klinik açıdan sağlıklı buzağı ve 1-3 aylık, 20 adet farklı cinsiyette T. vitulorum ile doğal enfekte toplam 30 adet montofon ırkı buzağı kullanıldı. Biyokimyasal analizler için kan örnekleri V. jugularis'ten alındı. Alınan kan örneklerinden bir miktarı tam kan olarak ayrıldıktan sonra geriye kalan kanların serumları elde edildi. Tam kan redükte glutatyon (GSH), serum malondialdehit (MDA), haptoglobin, albümin, aspartat amino transferaz (AST), alkalen fosfataz (ALP), üre, toplam protein ve demir (Fe) düzeyleri kolorimetrik yöntemlerle belirlendi. T. vitulorum ile doğal enfekte buzağılarda serum haptoglobin (P<0.01), AST, ALP ve MDA değerlerinin yükseldiği (P<0.05) albümin, toplam protein, Fe, GSH değerinin ise azaldığı belirlendi (P<0.05). Sonuç olarak, T. vitulorum ile enfekte buzağılarda akut faz yanıt oluştuğu ve bunun sonucu olarak da serum haptoglobin sentezinin arttığı, karaciğerin etkilenip fonksiyonlarının bozulduğu ve oksidatif stres oluştuğu belirlendi.Anahtar sözcükler: Akut faz proteinler, buzağı, oksidatif stres, Toxocara vitulorum. Investigation of the levels of serum haptoglobin, oxidative indicators and some biochemical parameters in calves naturally infected with Toxocara vitulorumSummary: The purpose of the study was to determine the levels of serum haptoglobin, oxidative indicators and some biochemical parameters in calves naturally infected with Toxocara vitulorum, and to investigate their diagnostic importance. For this purpose, 10 clinically healthy calves as control and 20 calves with naturally infected with T. vitulorum at 1-3 months of age with different gender total 30 Brown Swiss calves were used. Blood samples were collected from V. jugularis for biochemical analyses. Part of blood samples were stored without any process as a whole blood. The serum was seperated from remaining blood samples. Whole blood reduced glutathione (GSH), serum malondialdehyde (MDA), haptoglobin, albumin, aspartate amino transferase (AST), alkaline phosphatase (ALP), urea, total protein and iron (Fe) levels were determined by colorimetric methods. Levels of serum haptoglobin (P<0.01), AST, ALP and MDA (P<0.05) were determined to increase in blood whereas the levels of albumin, total protein, Fe, GSH (P<0.05) decreased in calves infected with T. vitulorum. In conclusion, the results indicated that the acute phase response was occured in calves infected with T. vitulorum, and as a results of this, the levels of serum haptoglobin synthesis increased, the functions of liver deteriorated and oxidative stress was amplified.

Effect of Vitamin C on Oxidant and Antioxidant Parameters in Cattle Immunized by Blackleg Vaccine

“…In studies conducted on cattle, MDA levels were reported to be affected in some bacterial, viral, and parasitic diseases. It is reported that the level of MDA increases in skin papilloma (Arslan et al, 2018), Fasciola gigantica (Bahrami et al, 2014), Neosporiosis (Glombowsky et al, 2017), Coccidiosis (Yilmaz andIssi, 2014), Babesiosis (Saleh, 2009), Anaplazmosis (Esmaeilnejad et al, 2018), Dictyocaulus viviparus (Deǧer et al, 2008), Hypoderma spp (Merhan et al, 2017), Theileriosis (Kızıl et al, 2011), Toxocara vitulorum (Bozukluhan et al, 2017), Hidatidosis (Heidarpour et al, 2013), Tuberculosis (Kızıl and Keltek, 2017), listeriosis (Jaguezeski et al, 2018), FMD (Khoshvaghti et al, 2014;Uzlu et al, 2016), Omphalitis (Bozukluhan et al, 2016), Brucellosis (Perin et al, 2017), Mastitis (Deveci and Güven, 2008), anthrax vaccine administration (Doğan et al, 2021), Dystocia (Bayyit and Merhan, 2020), and transplanted cattle (Chirase et al, 2004).…”

Determination of Levels of Some Acute Phase Proteins, Tumor Necrosis Factor-α, Interleukin-1 and Interleukin-6 in Cattle with Trichophythosis

“…SAA, which can increase over 1000 times in the circulation in inflammatory conditions, has functions such as preventing antibody formation by lymphocytes, inducing collagenase, and increasing leukocyte adhesion to endothelial cells (Petersen et al, 2004;Murata et al, 2004). The levels of SAA increases in bacterial (Merhan et al, 2017b), viral (Merhan et al, 2017c), and parasitic infections (Bozukluhan et al, 2017). In addition, serum levels increase in ketosis (Brodzki et al, 2021), after operations (Bozukluhan and Gökçe, 2007), and in fasting for more than 3 days (Katoh et al, 2002).…”