Toxin profile of Alexandrium ostenfeldii (Dinophyceae) from the Northern Adriatic Sea revealed by liquid chromatography–mass spectrometry

Ciminiello, Patrizia; Dell’Aversano, Carmela; Fattorusso, Ernesto; Magno, Silvana; Cangini, Monica; Pompei, Marinella; Guerrini, Franca; Boni, Laurita; Pistocchi, Rossella

doi:10.1016/j.toxicon.2006.02.003

Cited by 80 publications

(73 citation statements)

References 17 publications

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“…A. ostenfeldii is historically seen as background bloomer (Cembella et al, 2000;John et al, 2003), however, dense A. ostenfeldii blooms have been reported recently in the Narragansett Bay and the New River Estuary in the U.S. east coast (Borkman et al, 2012;Tomas et al, 2012), the Baltic Sea coast of Finland (Hakanen et al, 2012), along the Adriatic coast of Italy (Ciminiello et al, 2006), and recently in a creek of the Oosterschelde estuary in the Southwest of The Netherlands (Burson et al, 2014). This Oosterschelde estuary is a shallow, macrotidal and mesotrophic estuary with large stocks of mussels, oysters, and cockles ( Fig.…”

Section: Introductionmentioning

confidence: 99%

Characterization of multiple isolates from an Alexandrium ostenfeldii bloom in The Netherlands

et al. 2015

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Section: Introductionmentioning

confidence: 99%

Characterization of multiple isolates from an Alexandrium ostenfeldii bloom in The Netherlands

et al. 2015

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“…there is evidence that CIP toxins and their producers also are found in europe, the USA, and North Africa (Aasen et al, 2005a;Bire et al, 2002;Cembella and Krock, 2008;Ciminiello et al, 2006;John et al, 2003;MacKinnon et al, 2006;Marrouchi et al, 2010;Rundberget et al, 2011;touzet et al, 2008;Villar Gonzalez et al, 2006), and their presence, along with PSP to CTX also primarily in predatory fish consumed by humans (snapper, barracuda, amber jack and moray eel), and more investigations should be conducted on the role on Mtx in human intoxication.…”

Section: Ciguatera Fish Poisoning (Cfp) Toxinsmentioning

confidence: 99%

“…However, lC-MS/MS methods are presently refined that allow highly sensitive detection of GYM, Pntx, Pttx, and SPx (Aasen et al, 2005a;Alvarez et al, 2010;Bire et al, 2002;Cembella et al, 1999;Ciminiello et al, 2006;Fux et al, 2007;Gerssen et al, 2009a,b;MacKenzie et al, 2002;Quilliam et al, 2001;Stirling, 2001;takada et al, 2001a,b;Villar Gonzalez et al, 2006;Miles et al, 2010).…”

Section: Symptomsmentioning

confidence: 99%

A roadmap for hazard monitoring and risk assessment of marine biotoxins on the basis of chemical and biological test systems

Daneshian

2013

ALTEX

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Summary Aquatic food accounts for over 40% of global animal food products, and the potential contamination with toxins of algal origin -marine biotoxins -poses a health threat for consumers. The gold standards to assess toxins in aquatic food have traditionally been in vivo methodssingle marine biotoxins or combinations of marine biotoxins and intoxication symptomatology as well as monitoring the "success" of controls implemented during fishery and aquaculture production, processing, and retailing. For this reason, a workshop was organized in ermatingen, Switzerland by the Center for Alternatives to Animal testing -europe (CAAteurope) within the framework of the transatlantic think tank for toxicology (t 4 ).In contrast to other food products where hygiene and potential contamination with microbes or mold toxins is the prime issue, the emphasis in aquatic products (shellfish and finfish) is, beyond viral and bacterial contaminations, primarily placed on potential contamination with marine biotoxins owing to the numerous and recurring human intoxications.The gold standards to assess toxins in aquatic food have traditionally been in vivo methods, i.e., the mouse and the rat bioassays. Besides the ethical issues of in vivo bioassays there are specific difficulties, e.g., different exposure route (i.p. versus oral), low inter-species comparability, high intra-species variability, and questionable extrapolation of quantitative risk to humans, thus highlighting the need for the development of more reliable detection and quantification methods. Based on this reasoning, the european Food Safety Authority (eFSA) advocates the use of an analytical method, i.e., LC-MS (Liquid Chromatography coupled Mass Spectrometry), as a substitute for in vivo bioassays for almost all classes of marine toxins. However, although lC-MS is a very sensitive method that has the advantages of being able to detect multiple toxins in a single analysis and being good for confirming the identity of toxins, the quality of quantitative analysis is dependent on the availability of calibration standards. While many new toxin structural analogues have been detected and identified using LC-MS, this technique -as with most other methods -is not good at detecting new toxin types. When new toxin analogues are detected but accurate standards are not yet available, only an approximate quantitation is possible using estimated response factors.For the purpose of risk assessment of food, however, it is imperative to focus on the possible adverse effects, independent of whether they stem from one toxin or a combination of toxins. As toxicity is defined by functional and structural changes of biological systems, the development of a human-relevant in vitro system for appropriate risk assessment of marine biotoxins in seafood stands to reason. Moreover, poor correlation of human intoxications, of acute symptomatology and long-term adverse effects, and of predictive in vitro, in vivo, and analytical detection methodologies of marine biotoxins stems not least from a...

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“…Until recently, the cyclic imines could only be detected by mouse bioassay and LC-MS-based detection techniques [48, [121][122][123][124][125]. The only method currently available that has a biological component, besides the mouse bioassay, is a fluorescence polarization assay for the detection of gymnodimines and spirolides [126].…”

Section: Cyclic Iminesmentioning

confidence: 99%

Use of Biosensors as Alternatives to Current Regulatory Methods for Marine Biotoxins

Botana¹,

Vilariño²,

Alfonso³

et al. 2012

Springer Protocols Handbooks

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Toxin profile of Alexandrium ostenfeldii (Dinophyceae) from the Northern Adriatic Sea revealed by liquid chromatography–mass spectrometry

Cited by 80 publications

References 17 publications

Characterization of multiple isolates from an Alexandrium ostenfeldii bloom in The Netherlands

Characterization of multiple isolates from an Alexandrium ostenfeldii bloom in The Netherlands

A roadmap for hazard monitoring and risk assessment of marine biotoxins on the basis of chemical and biological test systems

Use of Biosensors as Alternatives to Current Regulatory Methods for Marine Biotoxins

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