Toxin-co-regulated pilus cluster in non-O1, non-toxigenic Vibrio cholerae: evidence of a third allele of pilin gene

Novais, Rogério Carlos

doi:10.1016/s0378-1097(98)00574-6

Cited by 27 publications

(35 citation statements)

References 17 publications

(21 reference statements)

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“…However, our results contradict the assumption that most cholera toxin-producing strains are also positive for TCP, since TCP is known to be the receptor for CTXφ infection of V. cholerae. In fact, our results appear to be consistent with data from recent studies that report the presence of the two virulence genes found in epidemic strains, ctxAB and tcpA, in environmental and clinical strain of serogroups other than O1 and O139 5,10,27,28,31,32 .…”

Section: Discussionsupporting

confidence: 93%

Distribution of virulence markers in clinical and environmental Vibrio cholerae non-O1/non-O139 strains isolated in Brazil from 1991 to 2000

Theophilo

Rodrigues

Leal

et al. 2006

Rev. Inst. Med. trop. S. Paulo

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SUMMARYOne hundred seventy nine Vibrio cholerae non-O1/non-O139 strains from clinical and different environmental sources isolated in Brazil from 1991 to 2000 were serogrouped and screened for the presence of four different virulence factors. The Random Amplification of Polymorphic DNA (RAPD) technique was used to evaluate the genetic relatedness among strains. Fifty-four different serogroups were identified and V. cholerae O26 was the most common (7.8%). PCR analysis for three genes (ctxA, zot, ace) located of the CTX genetic element and one gene (tcpA) located on the VPI pathogenicity island showed that 27 strains harbored one or more of these genes. Eight (4.5%) strains possessed the complete set of CTX element genes and all but one of these belonged to the O26 serogroup suggesting that V. cholerae O26 has the potential to be an epidemic strain. The RAPD profiles revealed a wide variability among strains and no genetic correlation was observed.

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Section: Discussionsupporting

confidence: 93%

Distribution of virulence markers in clinical and environmental Vibrio cholerae non-O1/non-O139 strains isolated in Brazil from 1991 to 2000

Theophilo

Rodrigues

Leal

et al. 2006

Rev. Inst. Med. trop. S. Paulo

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“…Likewise, two strains ± i.e., strain 151 (O34) and strain 208 (O27) isolated in Mexico and in Thailand in 1986 and 1992, respectively ± appear to be similar to these strains in that, although CT negative they were both tcpA and toxT positive [26]. Apart from the presence of a`free' RS1 element, strain AS480 was devoid of all known virulence genes associated with toxigenic V. cholerae.…”

Section: Discussionmentioning

confidence: 96%

Comparison of antibiogram, virulence genes, ribotypes and DNA fingerprints of Vibrio cholerae of matching serogroups isolated from hospitalised diarrhoea cases and from the environment during 1997–1998 in Calcutta, India

Chakraborty¹,

Garg²,

Ramamurthy³

et al. 2001

Journal of Medical Microbiology

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This study identi®ed 17 matching serogroups of Vibrio cholerae belonging to serogroups other than O1 and O139 isolated from human cases and from the environment during a concurrent clinical and environmental study conducted in Calcutta, a cholera endemic area. Isolates within these matching serogroups were compared by various phenotypic and genotypic traits to determine if the environment was the source of the organisms associated with the disease. Clinical strains of V. cholerae were resistant to a greater number of drugs and exhibited multi-drug resistance compared with their environmental counterparts. Except for the presence of the genes for the El Tor haemolysin and the regulatory element ToxR in most of the strains of V. cholerae examined, non-O1, non-O139 V. cholerae strains lacked most of the other known virulence traits associated with toxigenic V. cholerae O1 or O139. Restriction fragment-length polymorphism of virulence-associated genes, ribotypes and DNA ®ngerprints of strains of matched serogroups showed considerable diversity, although some gene polymorphisms and ribotypes of a few strains of different serogroups were similar. It is concluded that despite sharing the same serogroup, environmental and clinical isolates were genetically heterogeneous and were of different lineages.

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“…In a survey that was performed for the presence of virulence associated genes in non-O1, non-O139 V. cholerae strains, they found tcpA and the putative tcp gene cluster in two distinct non-toxigenic, non-O1/non-O139 strains. As this was an unexpected result, further investigation was carried out on these two isolates (Novais et al, 1999). in the study that was performed by Singh on Territorial waters of the Baltic Sea as a source of infections caused by V. cholera non-O1, non-O139, all isolates gave negative agglutination reactions with O1 and O139 antisera.…”

Section: Discussionmentioning

confidence: 99%

Identification of Vibrio cholerae pathogenicity island (ctxA, OmpW and tcpA) in non - O139 and non - O1 V. cholerae strains isolated from Karun River in Ahvaz, Iran

Sheikh¹,

Goodarzi²,

Aslani³

2012

Afr. J. Microbiol. Res.

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Vibrio cholerae is a usual inhabitant of the marine environment and causes severe diarrheal disease contaminating thousands of people each year in developing countries. The virulence of Vibrio spp. is regulated by the ctxAB and tcpA genes. These genes are supposed to be exclusively associated with clinical strains of O1 and O139 serogroups. In the present study, we examined the presence of virulence genes that contains OmpW, ctxA and tcpA of V. cholerae classical and Eltor variants in environmental strains of non-O1 and non O139 V. cholerae that were cultured seasonally from four sampling stations of the Karun River in Ahwaz. One hundred water samples were collected from four stations of Ahvaz Karun River. The colonies that were grown on TCBS confirmed to be Vibrio spp. by biochemical, serological and molecular methods. After DNA extraction, a polymeras chian reaction (PCR) assay was performed for detection of ctxA and tcpA (both Classical and ElTor variants) and OmpW was in the strain that was recognized as V. cholerae non -O139 and non -O1. Among 100 environmental samples of fresh water in this study, 27 isolate confirmed as V. cholerae spp. by PCR assay with ompW gene. Of among them, 4 isolate that were confirmed V. cholerae non O1-non O139 had gene for toxin coregulated pilli (Classical and Eltor). V. cholerae is one of the major causes of morbidity and mortality in the developing countries. The studies that were performed on V. cholera nonO1, nonO139 strains that usually do not carry the ctx genes coding for cholera toxin (CT) or toxinco regulated pilus which are the most important virulence characteristics of the cholera causing strains.

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Toxin-co-regulated pilus cluster in non-O1, non-toxigenic Vibrio cholerae: evidence of a third allele of pilin gene

Cited by 27 publications

References 17 publications

Distribution of virulence markers in clinical and environmental Vibrio cholerae non-O1/non-O139 strains isolated in Brazil from 1991 to 2000

Distribution of virulence markers in clinical and environmental Vibrio cholerae non-O1/non-O139 strains isolated in Brazil from 1991 to 2000

Comparison of antibiogram, virulence genes, ribotypes and DNA fingerprints of Vibrio cholerae of matching serogroups isolated from hospitalised diarrhoea cases and from the environment during 1997–1998 in Calcutta, India

Identification of Vibrio cholerae pathogenicity island (ctxA, OmpW and tcpA) in non - O139 and non - O1 V. cholerae strains isolated from Karun River in Ahvaz, Iran

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