Toxin accumulation, detoxification and oxidative stress in bivalve (Anomalocardia flexuosa) exposed to the dinoflagellate Prorocentrum lima

Leite, Isabel do Prado; Sandrini-Neto, Leonardo; Squella, Francisco José Lagreze; Alves, Thiago; Schramm, Mathias Alberto; Calado, Sabrina Loise de Morais; Assis, Helena Cristina Silva de; Mafra, Luiz L.

doi:10.1016/j.aquatox.2020.105738

Cited by 8 publications

(3 citation statements)

References 71 publications

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“…In this study, all three antioxidant enzymes (SOD, CAT, and GST) were generally stimulated in the gills of scallops, indicating that the antioxidant system was activated in response to the OA exposure, which may have caused oxidative stress to the scallops. Similar to our results, increased antioxidant enzymes activity was also observed in the bivalves Nodipecten subnodosus (Campa-Cordova et al, 2009) and Anomalocardia flexuosa (Leite et al, 2021), when exposed to Prorocentrum lima (a dinoflagellate producing DSP toxins).…”

Section: Oxidative Stress Biomarkerssupporting

confidence: 91%

iTRAQ-Based Quantitative Proteomic Analysis Reveals Toxicity Mechanisms in Chlamys farreri Exposed to Okadaic Acid

Wang

Zhang

et al. 2021

Front. Mar. Sci.

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Okadaic acid (OA), produced by dinoflagellates during harmful algal blooms, is a principal diarrhetic shellfish poisoning toxin. This toxin poses a potential threat to bivalves with economic values. To better understand the toxicity mechanism of OA to bivalves, in this study, oxidative stress biomarkers (superoxide dismutase, SOD; catalase, CAT; glutathione S-transferase, GST; malondialdehyde, MDA) and the expression of detoxification genes (heat shock protein 70, HSP70; heat shock protein 90, HSP90; cytochrome P450, CYP450) were assessed in the gills of scallops Chlamys farreri after 24 h, 48 h and 96 h exposure to OA. In addition, the digestive glands of scallops exposed to OA for 96 h were dissected for an iTRAQ based quantitative proteomic analysis. The results of OA exposure experiments showed that OA induces oxidative stress and significant enhancement of the expression of detoxification genes in scallops. The proteomics analysis revealed that 159 proteins altered remarkably in OA-treated scallops, and these proteins were involved in phagosomes, regulation of actin cytoskeleton, adherens junction, tight junction, and focal adhesion. Amino acid biosynthesis, carbon metabolism, pentose phosphate pathway, fructose and mannose metabolism in the digestive glands were also significantly impacted. Our data shed new insights on the molecular responses and toxicity mechanisms of C. farreri to OA.

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Section: Oxidative Stress Biomarkerssupporting

confidence: 91%

iTRAQ-Based Quantitative Proteomic Analysis Reveals Toxicity Mechanisms in Chlamys farreri Exposed to Okadaic Acid

Wang

Zhang

et al. 2021

Front. Mar. Sci.

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“…Several factors are believed to be important to establish an effective depuration process. These include the system design, initial water quality, oxygenation and flow rates, salinity, temperature, removal and settlement of fecal material and the period of purification (Lee and Younger, 2002). The depuration RAS system used in this study showed constant water quality parameters for each tested salinity, using a venturi injector allowed a high oxygen saturation due to the large flow of micro bubble.…”

Section: Discussionmentioning

confidence: 90%

Bioaccumulation and depuration of Escherichia coli in the tropical clam Anomalocardia brasiliana at different salinities

Squella

Sühnel

Ramos

et al. 2022

Arq. Bras. Med. Vet. Zootec.

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Anomalocardia brasiliana is an intertidal filter-feeding clam that can accumulate enterobacteria, such as Escherichia coli, and consequently affect human health. Shellfish depuration is a procedure which reduces microbiological contaminants; however, salinity and depuration time can vary across species to adequately reduce bacteria load. To analyze the effect of salinity on the bioaccumulation and depuration of E. coli by A. brasiliana, this study evaluated salinity and depuration time in animals artificially contaminated with E. coli. Each experimental group of clams were acclimated for 6 hours in a recirculating aquaculture system (RAS) and then exposed to E. coli for 18 hours. Following exposure, clams were then held at one of four salinities (35, 30, 25 e 20) for a period of one of four depuration times (0, 12, 24, 36 and 48h). The highest bioaccumulation of E. coli in A. brasiliana was observed in clams held at salinities of 35, 30 and 25. The greatest reduction of E. coli in A. brasiliana was observed in clams held at 25 for 48 hours. A salinity of 20 showed low bioaccumulation and depuration of E. coli. The results of this study will contribute to developing a protocol for depurating A. brasiliana to mitigate human health concerns.

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“…MLPs are transferred to higher trophic levels of the food web by predation, and thus the accumulation of MLPs is influenced by the feeding habits and habitat preferences of marine organisms . Moreover, some marine organisms can metabolize the toxin into less toxic compounds, while others may increase its toxicity. − The accumulation and elimination kinetics of MLPs are species- and toxin-specific. − Previous studies have reported that the orange-spotted grouper could contain a high capacity to oxidize Pacific-CTX-2 (P-CTX-2), Pacific-CTX-3 (P-CTX-3), and Pacific-CTX-4A/4B into the more potent Pacific-CTX-1 (P-CTX-1) Tridacna maxima, commonly known as giant clams, are recognized as a vector of CTXs.…”

Section: Introductionmentioning

confidence: 99%

The Prevalence of Marine Lipophilic Phycotoxins Causes Potential Risks in a Tropical Small Island Developing State

Zhu,

Li,

et al. 2024

Environ. Sci. Technol.

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Tropical small island developing states (SIDS), with their geographical isolation and limited resources, heavily rely on the fisheries industry for food and revenue. The presence of marine lipophilic phycotoxins (MLPs) poses risks to their economy and human health. To understand the contamination status and potential risks, the Republic of Kiribati was selected as the representative tropical SIDS and 55 species of 256 coral reef fish encompassing multiple trophic levels and feeding strategies were collected to analyze 17 typical MLPs. Our results showed that the potential risks of ciguatoxins were the highest and approximately 62% of fish species may pose risks for consumers. Biomagnification of ciguatoxins was observed in the food web with a trophic magnification factor of 2.90. Brevetoxin-3, okadaic acid, and dinophysistoxin-1 and -2 were first reported, but the risks posed by okadaic acid and dinophysistoxins were found to be negligible. The correlation analysis revealed that fish body size and trophic position are unreliable metrics to indicate the associated risks and prevent the consumption of contaminated fish. The potential risks of MLPs in Kiribati are of concern, and our findings can serve as valuable inputs for developing food safety policies and fisheries management strategies specific to tropical SIDS contexts.

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Toxin accumulation, detoxification and oxidative stress in bivalve (Anomalocardia flexuosa) exposed to the dinoflagellate Prorocentrum lima

Cited by 8 publications

References 71 publications

iTRAQ-Based Quantitative Proteomic Analysis Reveals Toxicity Mechanisms in Chlamys farreri Exposed to Okadaic Acid

iTRAQ-Based Quantitative Proteomic Analysis Reveals Toxicity Mechanisms in Chlamys farreri Exposed to Okadaic Acid

Bioaccumulation and depuration of Escherichia coli in the tropical clam Anomalocardia brasiliana at different salinities

The Prevalence of Marine Lipophilic Phycotoxins Causes Potential Risks in a Tropical Small Island Developing State

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