Toxigenic Species Aspergillus parasiticus Originating from Maize Kernels Grown in Serbia

Nikolić, Milica; Savic, Iva; Nikolić, Ana; Jauković, Marko; Kandić, Vesna; Stevanović, M.; Stankоvić, Slavica

doi:10.3390/toxins13120847

Cited by 7 publications

(8 citation statements)

References 33 publications

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“…The mature colonies were parsley green in color with yellow edges. Similar results were in a previous study conducted by Nikolić et al (2021). These results confirm the contamination of animal feed with A. parasiticus.…”

Section: Discussionsupporting

confidence: 92%

Molecular characterization of mycotoxin-producing Aspergillus parasiticus and sensitivity pattern to different disinfectants

Mughis¹,

Ali²,

Anjum³

et al. 2022

MJM

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Aims:The study was aimed to isolate and characterize the mycotoxin-producing filamentous Aspergillus parasiticus from the feed samples. The sensitivity pattern of the isolates was assessed against different disinfectants. Methodology and results: Fifty different feed samples were screened for A. parasiticus isolation. Isolates were subjected to macroscopic and microscopic characterization. Polymerase chain reaction was performed to confirm the isolates at the genomic level. Mycotoxin producing potential of the isolates was assessed by thin-layer chromatography (TLC). To quantify the toxins, high performance liquid (HPLC) was employed. The antifungal potential of disinfectants was determined by the well diffusion method followed by minimum inhibitory concentration (MIC) calculation. Out of twenty isolates of A. parasiticus, 11(55%) isolates were observed positive for toxin production. Three toxigenic isolates (AspP2, AspP4 and AspP8) were selected to evaluate their susceptibility against disinfectants by well diffusion method. AspP2 produced maximum (5.90 ng/mL) toxin, followed by AspP4 (3.11 ng/mL) and AspP8 (18.47 ng/mL). Terralin showed maximum fungicidal activity with 29.66 ± 8.08 mm zone of inhibition at 0.42 µg/mL MIC. Hypochlorite and Instru Star showed 99% disinfection with 30, 60 and 90 min contact time (6 mean log reduction) for all A. parasiticus isolates. Alpha Guard inhibited growth after 15 min contact time for all the isolates. Conclusion, significance and impact of study:This study provides data indicating the contamination of feed samples with mycotoxin-producing A. parasiticus isolates and their sensitivity against commercially available disinfectants. Use of these disinfectants in appropriate concentrations and time could help prevent the contamination of food, feed and healthcare settings with the fungal species.

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Section: Discussionsupporting

confidence: 92%

Molecular characterization of mycotoxin-producing Aspergillus parasiticus and sensitivity pattern to different disinfectants

Mughis¹,

Ali²,

Anjum³

et al. 2022

MJM

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show abstract

“…As an example, in a 5‐year study (2012–2016) carried out by Nikolić et al. (2021), A. parasiticus was detected on maize kernels under field conditions in Serbia. Before, in Serbia as well as in other European countries, the occurrence of A. parasiticus in maize seemed quite rare (Giorni et al., 2007).…”

Section: Resultsmentioning

confidence: 99%

“…As fungi present in A. section Flavi can be favored by warmer environmental conditions, it can be assumed that also other aflatoxigenic species different from A. flavus could be detected in a higher extent because of CC. As an example, in a 5-year study (2012-2016) carried out by Nikolić et al (2021), A. parasiticus was detected on maize kernels under field conditions in Serbia. Before, in Serbia as well as in other European countries, the occurrence of A. parasiticus in maize seemed quite rare (Giorni et al, 2007).…”

Section: 31mentioning

confidence: 99%

Changing climate, shifting mycotoxins: A comprehensive review of climate change impact on mycotoxin contamination

Casu,

Camardo Leggieri,

Toscano

et al. 2024

Comp Rev Food Sci Food Safe

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Climate change (CC) is a complex phenomenon that has the potential to significantly alter marine, terrestrial, and freshwater ecosystems worldwide. Global warming of 2°C is expected to be exceeded during the 21st century, and the frequency of extreme weather events, including floods, storms, droughts, extreme temperatures, and wildfires, has intensified globally over recent decades, differently affecting areas of the world. How CC may impact multiple food safety hazards is increasingly evident, with mycotoxin contamination in particular gaining in prominence. Research focusing on CC effects on mycotoxin contamination in edible crops has developed considerably throughout the years. Therefore, we conducted a comprehensive literature search to collect available studies in the scientific literature published between 2000 and 2023. The selected papers highlighted how warmer temperatures are enabling the migration, introduction, and mounting abundance of thermophilic and thermotolerant fungal species, including those producing mycotoxins. Certain mycotoxigenic fungal species, such as Aspergillus flavus and Fusarium graminearum, are expected to readily acclimatize to new conditions and could become more aggressive pathogens. Furthermore, abiotic stress factors resulting from CC are expected to weaken the resistance of host crops, rendering them more vulnerable to fungal disease outbreaks. Changed interactions of mycotoxigenic fungi are likewise expected, with the effect of influencing the prevalence and co‐occurrence of mycotoxins in the future. Looking ahead, future research should focus on improving predictive modeling, expanding research into different pathosystems, and facilitating the application of effective strategies to mitigate the impact of CC.

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“…The species of Aspergillus which are most relevant from a medical point of view are A. parasiticus and A. flavus, as they produce aflatoxins, secondary metabolites with harmful effects on both humans and animals [201], most notably carcinogenesis, mutagenesis, and teratogenesis [202]. Due to climate change, these species of Aspergillus can now be found in the soil of many countries worldwide, including Europe [203,204]. Considering that the use of harmful synthetic insecticides is common practice for eliminating A. parasiticus, the need for more environment-friendly methods of eradication has emerged [205].…”

Section: Antifungal Activity Against Aspergillus Parasiticusmentioning

confidence: 99%

Antimicrobial Properties of Capsaicin: Available Data and Future Research Perspectives

Periferakis,

Periferakis

et al. 2023

Nutrients

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Capsaicin is a phytochemical derived from plants of the genus Capsicum and subject of intensive phytochemical research due to its numerous physiological and therapeutical effects, including its important antimicrobial properties. Depending on the concentration and the strain of the bacterium, capsaicin can exert either bacteriostatic or even bactericidal effects against a wide range of both Gram-positive and Gram-negative bacteria, while in certain cases it can reduce their pathogenicity by a variety of mechanisms such as mitigating the release of toxins or inhibiting biofilm formation. Likewise, capsaicin has been shown to be effective against fungal pathogens, particularly Candida spp., where it once again interferes with biofilm formation. The parasites Toxoplasma gondi and Trypanosoma cruzi have been found to be susceptible to the action of this compound too while there are also viruses whose invasiveness is significantly dampened by it. Among the most encouraging findings are the prospects for future development, especially using new formulations and drug delivery mechanisms. Finally, the influence of capsaicin in somatostatin and substance P secretion and action, offers an interesting array of possibilities given that these physiologically secreted compounds modulate inflammation and immune response to a significant extent.

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Toxigenic Species Aspergillus parasiticus Originating from Maize Kernels Grown in Serbia

Cited by 7 publications

References 33 publications

Molecular characterization of mycotoxin-producing Aspergillus parasiticus and sensitivity pattern to different disinfectants

Molecular characterization of mycotoxin-producing Aspergillus parasiticus and sensitivity pattern to different disinfectants

Changing climate, shifting mycotoxins: A comprehensive review of climate change impact on mycotoxin contamination

Antimicrobial Properties of Capsaicin: Available Data and Future Research Perspectives

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