Toxigenic Corynebacteria: Adhesion, Invasion and Host Response

Ott, Lisa; Burkovski, Andreas

doi:10.1007/978-94-007-7624-1_8

Cited by 8 publications

(4 citation statements)

References 112 publications

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“…Furthermore, the bacteria were able to invade the cytoplasm of epithelial cells. Compared with adhesion rates found for different C. diphtheriae strains (for review, see Ott & Burkovski, 2014) and invasion rates determined (e.g. Ott et al, 2013), C. ulcerans rates observed here were at least two-to fivefold higher, indicating the high infectious potential of this pathogen.…”

Section: Discussionmentioning

confidence: 90%

Colonization of human epithelial cell lines by Corynebacterium ulcerans from human and animal sources

et al. 2015

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Corynebacterium ulcerans is an emerging pathogen transmitted by a zoonotic pathway to humans. Despite rising numbers of infections and potentially fatal outcomes, data on the colonization of the human host are lacking up to now. In this study, adhesion of two C. ulcerans isolates to human epithelial cells, invasion of host cells and the function of two putative virulence factors with respect to these processes were investigated. C. ulcerans strains BR-AD22 and 809 were able to adhere to Detroit562 and HeLa cells, and invade these epithelial cell lines with a rate comparable to other pathogens as shown by scanning electron microscopy, fluorescence microscopy and replication assays. Infection led to detrimental effects on the cells as deduced from measurements of transepithelial resistance. Mutant strains of putative virulence factors phospholipase D and DIP0733 homologue CULC22_00609 generated in this study showed no influence on colonization under the experimental conditions tested. The data presented here indicate a high infectious potential of this emerging pathogen.

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Section: Discussionmentioning

confidence: 90%

Colonization of human epithelial cell lines by Corynebacterium ulcerans from human and animal sources

et al. 2015

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“…diphtheria and C . ulcerans [ 86 ]. Different sialidases in a bacterium can have differences in their substrate specificities and could play important roles in the interaction with other organisms or in the infection of a specific tissue [ 66 ].…”

Section: Resultsmentioning

confidence: 99%

Rapidly evolving changes and gene loss associated with host switching in Corynebacterium pseudotuberculosis

et al. 2018

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Phylogenomics and genome scale positive selection analyses were performed on 29 Corynebacterium pseudotuberculosis genomes that were isolated from different hosts, including representatives of the Ovis and Equi biovars. A total of 27 genes were identified as undergoing adaptive changes. An analysis of the clades within this species and these biovars, the genes specific to each branch, and the genes responding to selective pressure show clear differences, indicating that adaptation and specialization is occurring in different clades. These changes are often correlated with the isolation host but could indicate responses to some undetermined factor in the respective niches. The fact that some of these more-rapidly evolving genes have homology to known virulence factors, antimicrobial resistance genes and drug targets shows that this type of analysis could be used to identify novel targets, and that these could be used as a way to control this pathogen.

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“…Within the phagolysosome bacteria are killed by production of reactive oxygen species (ROS) [ 11 ]. Many bacteria evolved strategies to resist to this specific stress from macrophages [ 38 , 72 , 73 ]. For example, SodA protects Salmonella Typhimurium from killing by macrophages within the early infection stage [ 74 ].…”

Section: Discussionmentioning

confidence: 99%

Corynebacterium diphtheriae Proteome Adaptation to Cell Culture Medium and Serum

et al. 2021

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Host-pathogen interactions are often studied in vitro using primary or immortal cell lines. This set-up avoids ethical problems of animal testing and has the additional advantage of lower costs. However, the influence of cell culture media on bacterial growth and metabolism is not considered or investigated in most cases. To address this question growth and proteome adaptation of Corynebacterium diphtheriae strain ISS3319 were investigated in this study. Bacteria were cultured in standard growth medium, cell culture medium, and fetal calf serum. Mass spectrometric analyses and label-free protein quantification hint at an increased bacterial pathogenicity when grown in cell culture medium as well as an influence of the growth medium on the cell envelope.

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Toxigenic Corynebacteria: Adhesion, Invasion and Host Response

Cited by 8 publications

References 112 publications

Colonization of human epithelial cell lines by Corynebacterium ulcerans from human and animal sources

Colonization of human epithelial cell lines by Corynebacterium ulcerans from human and animal sources

Rapidly evolving changes and gene loss associated with host switching in Corynebacterium pseudotuberculosis

Corynebacterium diphtheriae Proteome Adaptation to Cell Culture Medium and Serum

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