Toxigenic and non-toxigenic Pasteurella multocida genotypes, based on capsular, LPS, and virulence profile typing, associated with pneumonic pasteurellosis in Iran

Mombeni, Ehsan Gharib; Gharibi, Darioush; Ghorbanpoor, Masoud; Jabbari, Ahmad Reza; Hierro, Eva

doi:10.1016/j.vetmic.2021.109077

Cited by 7 publications

(6 citation statements)

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“…The prevalence of capsular type A in cat isolates aligns with previous studies (Arumugam et al, 2011;Aski & Tabatabaei, 2016;Ferreira et al, 2015). Moreover, in studies conducted by Prabhakar et al (2012), Ewers et al (2006), Mombeni et al (2021), Kumar et al (2004) and Aski and Tabatabaei (2016), it was reported that CapA is more commonly found than CapD in mammal isolates. Additionally, Wilson and Ho (2013) found that serogroups A and D are responsible for the majority of human infections.…”

Section: Discussionsupporting

confidence: 89%

Molecular characterization of Pasteurella multocida from cats and antibiotic sensitivity of the isolates

Ziagham,

Gharibi,

Mosallanejad

et al. 2024

Veterinary Medicine & Sci

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BackgroundCompanion animals, including dogs and cats, are frequently identified as sources of Pasteurella multocida, a bacterium that can be transmitted to humans and cause infections.ObjectivesThis survey defines the prevalence, antibiotic sensitivity, capsular types, lipopolysaccharide (LPS) types and virulence factors of P. multocida isolated from cats.MethodsA total of 100 specimens from various cat breeds were collected. P. multocida was characterized using both biochemical tests and PCR. Genotypes of isolates were determined using capsular and LPS typing methods. Additionally, virulotyping was performed by detecting the presence of 12 virulence‐associated genes. Disk diffusion was used to determine the antibiotic sensitivity of the isolates.ResultsThe prevalence of P. multocida in cats was 29%. Among the isolates, the majority were capsular type A (96.5%) and type D (3.4%), with a predominant presence of type A. Twenty‐six of the isolates (89.66%) belonged to LPS genotype L6, whereas three isolates (10.3%) belonged to genotype L3. Among the 12 virulence genes examined, sodC, oma87, ptfA, nanB and ompH showed remarkable prevalence (100%). The toxA gene was detected in four isolates (13.8%). Variations were observed in other virulence genes. The nanH gene was present in 93.1% of the isolates, whereas the pfhA gene was detected in 58.6% of the isolates. The exbD‐tonB, hgbB, sodA and hgbA genes showed prevalence rates of 96.5%, 96.5%, 96.5% and 82.8%, respectively. Additionally, particular capsule and LPS types were associated with specific virulence genes. Specifically, the toxA and pfhA genes were found to be more prevalent in isolates with capsular type A and LPS genotype L6. Most isolates were resistant to ampicillin, clindamycin, lincomycin, streptomycin and penicillin.ConclusionsAccording to this epidemiological and molecular data, P. multocida from cats possess several virulence‐associated genes and are resistant to antimicrobial medicines commonly used in humans and animals. Thus, it is crucial to consider the public health concerns of P. multocida in humans.

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Section: Discussionsupporting

confidence: 89%

Molecular characterization of Pasteurella multocida from cats and antibiotic sensitivity of the isolates

Ziagham,

Gharibi,

Mosallanejad

et al. 2024

Veterinary Medicine & Sci

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“…A compatible result was also observed in a study conducted by Aski and Tabatabaei [ 30 ], in which the prevalence of capA , capB , and capD genes in P. multocida isolates from sheep was 45.45, 13.63, and 34.09%, respectively. However, in some cases, the capB gene was absent in P. multocida isolates from sheep [ 13 , 15 ]. Nevertheless, data in the literature suggest that capsular types A and D are common among P. multocida strains isolated from sheep and goats [ 13 , 15 , 29 , 30 ].…”

Section: Resultsmentioning

confidence: 99%

“…However, in some cases, the capB gene was absent in P. multocida isolates from sheep [ 13 , 15 ]. Nevertheless, data in the literature suggest that capsular types A and D are common among P. multocida strains isolated from sheep and goats [ 13 , 15 , 29 , 30 ].…”

Section: Resultsmentioning

confidence: 99%

“…In sheep, serotype A, particularly sub-serotype A1 and A3, is more predominant than D or B serotypes [ 11 , 12 ]. Besides cellular capsules, some virulence factors required for in vivo reproduction and disease development (lipopolysaccharide) or cellular survival (iron-regulated or iron-acquisition proteins) have been identified in P. multocida as well as other virulence-associated factors such as toxins, fimbriae, adhesins, sialic acid metabolism, hyaluronidase, or outer membrane proteins [ 13 – 15 ].…”

Section: Introductionmentioning

confidence: 99%

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First study on capsular serotypes and virulence factors of Pasteurella multocida isolates from Phan Rang sheep in Vietnam

Nguyen

Huy

et al. 2023

Vet World

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Background and Aim: Pasteurella multocida is considered as a main factor mediating pneumonic pasteurellosis in ruminants, including sheep. It is also a current threat to Phan Rang sheep in Vietnam. This study aimed to characterize P. multocida isolated from Phan Rang sheep, their antibiotic resistance profile, and the prevalence of some virulence-associated genes of these strains. Materials and Methods: Bacteria were isolated on brain heart infusion, 10% sheep blood agar plates, and screened by biochemical tests. The polymerase chain reaction technique was used with specific primers to identify P. multocida, the presence of virulence-associated genes, and serotypes of isolates. Antimicrobial susceptibility and biofilm formation of isolates were examined using the disk diffusion method and crystal violet-based method, respectively. Results: A total of 41 P. multocida strains were isolated from 485 samples from clinically sick and healthy sheep. Of the isolates, 58.53% were serotype A, 9.75% were serotype B, and 31.71% were serotype D. Healthy animals were infected with serotype D only. All 15 virulence genes were identified in all strains isolated from clinically sick sheep, while strains isolated from healthy sheep carried 11/15 virulence genes tested. Among virulence-associated genes exbB, exbD, tonB, ompA, oma87, fimA, hgbA, and nanB were detected in over 90% of isolates, whereas hgbB, nanH, tbpA and pfhA were less frequent. Interestingly, pmHAS and tadD were highly prevalent in capsular type A strains, whereas the toxA gene was detected in capsular type D strains only. All of the isolated strains were fully susceptible to enrofloxacin, ciprofloxacin, neomycin, and ofloxacin. About 92.68% were susceptible to chloramphenicol and 90.24% to amikacin, but there was high resistance to erythromycin, tetracycline, and amoxicillin. Our results reveal that 53.65% of 41 isolates could produce biofilm, whereas 46.34% could not. Conclusion: Pasteurella multocida from Phan Rang sheep possess many virulence genes and resistance to several common antibiotics such as erythromycin, tetracycline, and amoxicillin. The results are an important warning regarding antibiotic resistance of P. multocida.

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“…Based on its capsular antigen, P. multocida is grouped into five serotypes: A, B, D, E and F [ 3 ]. Goats are the preferred hosts for P. multocida serotype A and D strains [ 4 ]. The capsular antigen of serotype A, mainly composed of hyaluronic acid, promotes the colonization of P. multocida in the mucosa of the lower respiratory tract [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

Activation of MyD88-Dependent TLR Signaling Modulates Immune Response of the Mouse Heart during Pasteurella multocida Infection

Jiang

et al. 2023

Microorganisms

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Pasteurella multocida (P. multocida) is an important zoonotic pathogen. In addition to lung lesions, necropsies have revealed macroscopic lesions in the heart in clinical cases. However, most previous studies focused on lung lesions while ignoring heart lesions. Therefore, to investigate the immune response of the P. multocida-infected heart, two murine infection models were established by using P. multocida serotype A (Pm HN02) and D (Pm HN01) strains. Histopathological examination revealed heterogeneous inflammatory responses, including immune cell infiltration in the epicardial and myocardial areas of the heart. Transcriptome sequencing was performed on infected cardiac tissues. To explore the traits of immune responses, we performed the functional enrichment analysis of differentially expressed genes, gene set enrichment analysis and gene set variation analysis. The results showed that the innate immune pathways were significantly regulated in both groups, including the NOD-like receptor signaling pathway, the complement and coagulation cascade and cytokine–cytokine receptor interaction. The Toll-like receptor signaling pathway was only significantly activated in the Pm HN02 group. For the Pm HN02 group, immunohistochemistry analysis further verified the significant upregulation of the hub component MyD88 at the protein level. In conclusion, this study reveals critical pathways for host heart recognition and defense against P. multocida serotypes A and D. Moreover, MyD88 was upregulated by P. multocida serotype A in the heart, providing a theoretical basis for future prevention, diagnosis and treatment research.

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Toxigenic and non-toxigenic Pasteurella multocida genotypes, based on capsular, LPS, and virulence profile typing, associated with pneumonic pasteurellosis in Iran

Cited by 7 publications

References 37 publications

Molecular characterization of Pasteurella multocida from cats and antibiotic sensitivity of the isolates

Molecular characterization of Pasteurella multocida from cats and antibiotic sensitivity of the isolates

First study on capsular serotypes and virulence factors of Pasteurella multocida isolates from Phan Rang sheep in Vietnam

Activation of MyD88-Dependent TLR Signaling Modulates Immune Response of the Mouse Heart during Pasteurella multocida Infection

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