Toxicology and Drug Interactions of Nutraceuticals

Cope, Rhian B.

doi:10.1007/978-3-030-04624-8_51

Cited by 2 publications

(1 citation statement)

References 340 publications

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“…Some smaller compounds were also positively correlated with AhR activity, with the highest correlation for C 10 H 10 O 2 (R 2 = 0.74) assigned to methoxy cinnamaldehyde based on its relatively clean MS 2 spectrum. The interaction of methoxy cinnamaldehyde with the AhR-responsive gene product CYP1A1 results in its oxidation into an acid (Hasegawa et al, 2002), and it appears to be mainly protective (Cope, 2019). This would be consistent with the observed anticorrelation of this product with cell toxicity (R 2 = 0.42).…”

Section: Obssupporting

confidence: 69%

Relationship between the molecular composition, visible light absorption, and health-related properties of smoldering woodsmoke aerosols

et al. 2020

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Abstract. Organic aerosols generated from the smoldering combustion of wood critically impact air quality and health for billions of people worldwide; yet, the links between the chemical components and the optical or biological effects of woodsmoke aerosol (WSA) are still poorly understood. In this work, an untargeted analysis of the molecular composition of smoldering WSA, generated in a controlled environment from nine types of heartwood fuels (African mahogany, birch, cherry, maple, pine, poplar, red oak, redwood, and walnut), identified several hundred compounds using gas chromatography mass spectrometry (GC-MS) and nano-electrospray high-resolution mass spectrometry (HRMS) with tandem multistage mass spectrometry (MSn). The effects of WSA on cell toxicity as well as gene expression dependent on the aryl hydrocarbon receptor (AhR) and estrogen receptor (ER) were characterized with cellular assays, and the visible mass absorption coefficients (MACvis) of WSA were measured with ultraviolet–visible spectroscopy. The WSAs studied in this work have significant levels of biological and toxicological activity, with exposure levels in both an outdoor and indoor environment similar to or greater than those of other toxicants. A correlation between the HRMS molecular composition and aerosol properties found that phenolic compounds from the oxidative decomposition of lignin are the main drivers of aerosol effects, while the cellulose decomposition products play a secondary role; e.g., levoglucosan is anticorrelated with multiple effects. Polycyclic aromatic hydrocarbons (PAHs) are not expected to form at the combustion temperature in this work, nor were they observed above the detection limit; thus, biological and optical properties of the smoldering WSA are not attributed to PAHs. Syringyl compounds tend to correlate with cell toxicity, while the more conjugated molecules (including several compounds assigned to dimers) have higher AhR activity and MACvis. The negative correlation between cell toxicity and AhR activity suggests that the toxicity of smoldering WSA to cells is not mediated by the AhR. Both mass-normalized biological outcomes have a statistically significant dependence on the degree of combustion of the wood. In addition, our observations support the fact that the visible light absorption of WSA is at least partially due to charge transfer effects in aerosols, as previously suggested. Finally, MACvis has no correlation with toxicity or receptor signaling, suggesting that key chromophores in this work are not biologically active on the endpoints tested.

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Section: Obssupporting

confidence: 69%