Toxicological studies on stem bark, leaf and seed kernel of yellow oleander (Thevetia peruviana)

Oji, Okoro; Okafor, Queen E

doi:10.1002/(sici)1099-1573(200003)14:2<133::aid-ptr598>3.0.co;2-k

Cited by 35 publications

(26 citation statements)

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“…It is a medicinal plant used to treat different diseases, including cancer [27]. The leaves of yellow oleander had previously been studied and shown to possess antimicrobial, antifungal, antidiarrheal, insecticide, molluscacide, and rodenticide activity [8, 28–30]. However, scientific evidence to demonstrate mode of action, targets and agents responsible for the bioactivity in the fruit is still needed.…”

Section: Discussionmentioning

confidence: 99%

“…K. Schum, also known as Cascabela peruviana (L.) Lippold, yellow oleander or lucky nut in the West Indies, shows a diverse array of properties ranging from being a cardiotonic to a toxin. T. peruviana plant has been used traditionally for the treatment of gastrointestinal and inflammatory diseases, heart failures and skin tumors [7, 8]. All parts of the plant are poisonous due to the presence of cardiac toxins, but the fruit of T. peruviana , is the most toxic part of the plant because it has the highest and diverse content of cardiac glycosides.…”

Section: Introductionmentioning

confidence: 99%

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Anticancer potential of Thevetia peruviana fruit methanolic extract

Ramos-Silva

Tavares-Carreón

Figueroa

et al. 2017

BMC Complement Altern Med

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Background Thevetia peruviana (Pers.) K. Schum or Cascabela peruviana (L.) Lippold (commonly known as ayoyote, codo de fraile, lucky nut, or yellow oleander), native to Mexico and Central America, is a medicinal plant used traditionally to cure diseases like ulcers, scabies, hemorrhoids and dissolve tumors. The purpose of this study was to evaluate the cytotoxic, antiproliferative and apoptotic activity of methanolic extract of T. peruviana fruits on human cancer cell lines.MethodsThe cytotoxic activity of T. peruviana methanolic extract was carried out on human breast, colorectal, prostate and lung cancer cell lines and non-tumorigenic control cells (fibroblast and Vero), using the MTT assay. For proliferation and motility, clonogenic and wound-healing assays were performed. Morphological alterations were monitored by trypan blue exclusion, as well as DNA fragmentation and AO/EB double staining was performed to evaluate apoptosis. The extract was separated using flash chromatography, and the resulting fractions were evaluated on colorectal cancer cells for their cytotoxic activity. The active fractions were further analyzed through mass spectrometry.ResultsThe T. peruviana methanolic extract exhibited cytotoxic activity on four human cancer cell lines: prostate, breast, colorectal and lung, with values of IC50 1.91 ± 0.76, 5.78 ± 2.12, 6.30 ± 4.45 and 12.04 ± 3.43 μg/mL, respectively. The extract caused a significant reduction of cell motility and colony formation on all evaluated cancer cell lines. In addition, morphological examination displayed cell size reduction, membrane blebbing and detachment of cells, compared to non-treated cancer cell lines. The T. peruviana extract induced apoptotic cell death, which was confirmed by DNA fragmentation and AO/EB double staining. Fractions 4 and 5 showed the most effective cytotoxic activity and their MS analysis revealed the presence of the secondary metabolites: thevetiaflavone and cardiac glycosides.Conclusion T. peruviana extract has potential as natural anti-cancer product with critical effects in the proliferation, motility, and adhesion of human breast and colorectal cancer cells, and apoptosis induction in human prostate and lung cancer cell lines, with minimal effects on non-tumorigenic cell lines.Electronic supplementary materialThe online version of this article (doi:10.1186/s12906-017-1727-y) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Anticancer potential of Thevetia peruviana fruit methanolic extract

Ramos-Silva

Tavares-Carreón

Figueroa

et al. 2017

BMC Complement Altern Med

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“…It is reported that, in addition to the cardiotonic effect of T. peruviana , there is an important diuretic effect that may explain the weight loss of the treated groups. [24]…”

Section: Discussionmentioning

confidence: 99%

Increased acetylcholine esterase activity produced by the administration of an aqueous extract of the seed kernel of Thevetia peruviana and its role on acute and subchronic intoxication in mice

et al. 2014

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Background:The real mechanism for Thevetia peruviana poisoning remains unclear. Cholinergic activity is important for cardiac function regulation, however, the effect of T. peruviana on cholinergic activity is not well-known.Objective:To study the effect of the acute administration of an aqueous extract of the seed kernel of T. peruviana on the acetylcholine esterase (AChE) activity in CD1 mice as well its implications in the sub-chronic toxicity of the extract.Materials and Methods:A dose of 100 mg/kg of the extract was administered to CD1 mice and after 7 days, serum was obtained for ceruloplasmin (CP) quantitation and liver function tests. Another group of mice received a 50 mg/kg dose of the extract 3 times within 1 h time interval and AChE activity was determined for those animals. Heart tissue histological preparation was obtained from a group of mice that received a daily 50 mg/kg dose of the extract by a 30-days period.Results:CP levels for the treated group were higher than those for the control group (Student's t-test, P ≤ 0.001). AChE activity in the treated group was significantly higher than the control group (Tukey test, control vs. T. peruviana, P ≤ 0.001). Heart tissue histological preparations showed leukocyte infiltrates and necrotic areas, consistent with infarcts.Conclusion:The increased levels of AChE and the hearth tissue infiltrative lesions induced by the aqueous seed kernel extract of T. peruviana explains in part the poisoning caused by this plant, which can be related to an inflammatory process.

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“…Leaf and seed extracts are toxic against Tribolium castaneum [3][4][5] . Seeds, leaves, fruits and roots of Thevetia peruviana (Pers) are considered as potential sources of biologically active compounds, such as insecticides, rodenticide, fungicide and bactericides [6][7][8][9][10][11][12][13][14][15] . The plants toxins have tested in experiments for the uses of biological pest control.…”

Section: Introductionmentioning

confidence: 99%

Investigation on Effect of Thevetia Peruviana (Pers) on the Mortality of Holotrichia Serrata (Fab) Adults (Coleoptera: Scarabaiedae)

Theurkar¹,

Patil²,

Ghadage³

et al. 2014

Int. Res. J. Pharm.

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White grub is called as Chaffer beetle. White grub is a National pest, it has worldwide distribution. The white grub's damage agricultural crops and there adults were feed on leaves of economical and medically important host plants like Azadirachta indica, Acacia arabica, Ziziphus zizyphus and Acacia catechu. The Holotrichia serrata (Fab.), Holotrichia fissa (Br.) and Leucopholis lepidophora (Bl.) are important agriculture pest cause damage to commercial crops such as groundnut, potato, pea, maize, sugarcane etc. The bio pesticides or insecticides, an alternative to systematic insecticides and are organic, often low mammalian toxicity and less hazardous to the environment. In present study, the leaf of Thevetia peruviana (Pers.) or Yellow Oleander were extracted in aqueous and treated against the adults of Holotrichia serrata (Fab.). The tested aqueous leaf extract effectively produced 50 % mortality of Holotrichia serrata (Fab.) and their toxicity was 0.025 % after 48 hours bioassay.

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Toxicological studies on stem bark, leaf and seed kernel of yellow oleander (Thevetia peruviana)

Cited by 35 publications

References 4 publications

Anticancer potential of Thevetia peruviana fruit methanolic extract

Anticancer potential of Thevetia peruviana fruit methanolic extract

Increased acetylcholine esterase activity produced by the administration of an aqueous extract of the seed kernel of Thevetia peruviana and its role on acute and subchronic intoxication in mice

Investigation on Effect of Thevetia Peruviana (Pers) on the Mortality of Holotrichia Serrata (Fab) Adults (Coleoptera: Scarabaiedae)

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