Toxicological interactions of silver nanoparticles and non-essential metals in human hepatocarcinoma cell line

Miranda, Renata Rank; Bezerra, Arandi Ginane; Ribeiro, Ciro Alberto de Oliveira; Randi, Marco Antônio Ferreira; Voigt, Carmen Lúcia; Skytte, Lilian; Rasmussen, Kaare Lund; Kjeldsen, Frank; Neto, Francisco Filipak

doi:10.1016/j.tiv.2017.01.003

Cited by 33 publications

(29 citation statements)

References 51 publications

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“…Considering that ATP production was hampered by the exposure, cells may not have undergone apoptosis, at least in the AgNP þ Cd 2þ group, which may explain the marked increase in LDH leakage (characteristic of necrosis). In addition, we previously reported a change in the cell death mechanism from apoptosis to necrosis using timelapse confocal microscopy in HepG2 cells exposed to AgNP þ Cd 2þ (Miranda et al 2017). The findings in this study validate that co-exposure to AgNP and Cd induces significantly more harm to HepG2 cells than exposure to either of the contaminants singly.…”

Section: Cell Viability and Energy Homeostasissupporting

confidence: 82%

“…Comparison of viability data of the present study and in Miranda et al (2017) suggests that toxicological interaction of AgNP with Cd 2þ depends on the particle's characteristics, such as coating and size, as well as the period of exposure. Noncoated 2 nm AgNP (Miranda et al 2017) interaction with Cd 2þ induced earlier toxic effects (e.g.…”

Section: Cell Viability and Energy Homeostasissupporting

confidence: 53%

“…Contaminant concentrations used in the study were selected based on a previous study published by our group (Miranda et al 2017). Briefly, toxicity-screening tests of AgNP and Cd 2þ were made to identify and select two concentrations of each contaminant; a non-cytotoxic one and a cytotoxic one.…”

Section: Exposure Protocolmentioning

confidence: 99%

“…decreased cell viability and proliferation, and increased production of reactive oxygen species [ROS]), even though AgNP did not increase intracellular Cd 2þ levels (Miranda et al 2017). Based on these observations, we used mass spectrometry-based proteomics in this study to elucidate in greater detail the molecular processes underlying the toxic biological outcomes induced by a combined exposure of AgNP and Cd 2þ in HepG2 cells.…”

Section: Introductionmentioning

confidence: 99%

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Co-exposure to silver nanoparticles and cadmium induce metabolic adaptation in HepG2 cells

et al. 2018

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(2018) Co-exposure to silver nanoparticles and cadmium induce metabolic adaptation in HepG2 cells, Nanotoxicology, 12:7, 781-795, DOI: 10.1080/17435390.2018 Although multiple studies have reported the toxicological effects and underlying mechanisms of toxicity of silver nanoparticles (AgNP) in a variety of organisms, the interactions of AgNP with environmental contaminants such as cadmium are poorly understood. We used biochemical assays and mass spectrometry-based proteomics to assess the cellular and molecular effects induced by a co-exposure of HepG2 cells to AgNP and cadmium. Cell viability and energy homeostasis were slightly affected after a 4-h exposure to AgNP, cadmium, or a combination of the two; these endpoints were substantially altered after a 24-h co-exposure to AgNP and cadmium, while exposure to one of the two contaminants led only to minor changes. Proteomics analysis followed the same trend: while a 4-h exposure induced minor protein deregulation, a 24-h exposure to a combination of AgNP and cadmium deregulated 43% of the proteome. The toxicity induced by a combined exposure to AgNP and cadmium involved (1) inactivation of Nrf2, resulting in downregulation of antioxidant defense and proteasome-related proteins, (2) metabolic adaptation and ADP/ATP imbalance, and (3) increased protein synthesis possibly to reestablish homeostasis. The adaptation strategy was not sufficient to restore ADP/ATP homeostasis and to avoid cell death. ARTICLE HISTORY

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Section: Cell Viability and Energy Homeostasissupporting

confidence: 82%

Section: Cell Viability and Energy Homeostasissupporting

confidence: 53%

Section: Exposure Protocolmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Co-exposure to silver nanoparticles and cadmium induce metabolic adaptation in HepG2 cells

et al. 2018

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“…It is thus possible to distinguish between viable and dead cells [79,80]. In a study, endosome-lysosome system stability which was measured by neutral red assay decreased after exposure to the silver nanoparticle (30%) [81]. Another method is called Trypan blue exclusion test.…”

Section: Necrosis Assaymentioning

confidence: 99%

In vitro and in vivo toxicity assessment of nanoparticles

2017

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Nanotechnology has revolutionized gene therapy, diagnostics and environmental remediation. Their bulk production, uses and disposal have posed threat to the environment. With the appearance of these nanoparticles in the environment, their toxicity assessment is an immediate concern. This review is an attempt to summarize the major techniques used in cytotoxity determination. The review also presents a detailed and elaborative discussion on the toxicity imposed by different types of nanoparticles including carbon nanotubes, gold nanoparticles, silver nanoparticles, quantum dots, fullerenes, aluminium nanoparticles, zinc nanoparticles, iron nanoparticles, titanium nanoparticles and silica nanoparticles. It discusses the in vitro and in vivo toxological effects of nanoparticles on bacteria, microalgae, zebrafish, crustacean, fish, rat, mouse, pig, guinea pig, human cell lines and human. It also discusses toxological effects on organs such as liver, kidney, spleen, sperm, neural tissues, liver lysosomes, spleen macrophages, glioblastoma cells, hematoma cells and various mammalian cell lines. It provides information about the effects of nanoparticles on the gene-expression, growth and reproduction of the organisms.

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