Toxicological in vitro effects of heavy metals on gilthead seabream (Sparus aurata L.) head–kidney leucocytes

Morcillo, Patricia; Cordero, Héctor; Meseguer, José; Esteban, M. Ángeles

doi:10.1016/j.tiv.2015.09.021

Cited by 33 publications

(16 citation statements)

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“…SOD(Mn) and SOD(Cu) gene transcription levels were not significantly modulated in medaka larvae and embryos following exposure to Cd-spiked sediments [21]. Similar results were reported in isolated leukocytes exposed to higher concentrations of Cd but for shorter times (5.6 mg/L for two hours), while CAT mRNA expression was enhanced [51]. Indeed, CAT protein has been described as a sensitive biomarker to toxic metals [52].…”

Section: Redox Homeostasis Disturbancesupporting

confidence: 76%

New Insights into Cellular Impacts of Metals in Aquatic Animals

et al. 2020

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Toxic metals remain a current important threat to aquatic ecosystems, despite regulatory efforts to reduce their release. Several toxic metals already appear in the list of priority substances polluting surface waters, while concerns arise from the increasing use of technology-critical metals such as metallic nanoparticles, rare-earth, and platinum group metals. In aquatic environments, various chemical, biological and physical processes determine the impact of metals on the biota. This review provides insights into responses to toxic metals recently reported in freshwater and marine animals. The specific emphasis is on: (i) common cellular and molecular responses; (ii) stress proteins; (iii) redox homeostasis; (iv) cytoskeleton rearrangement; (v) metabolism reshuffle; (vi) free cellular energy and mitochondrial metabolism; and (vi) immunity. These endpoints are promising, notably in multi-biomarker approaches to identify precise cellular toxicity pathways and anticipate the impact of environmental metal pollution.

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Section: Redox Homeostasis Disturbancesupporting

confidence: 76%

New Insights into Cellular Impacts of Metals in Aquatic Animals

et al. 2020

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“…Pericardial edema may be a general response under a toxic environment. It has been reported that pericardial edema occurred in zebrafish embryos exposed to different toxicants, including organic pollutants (Hill et al ., ; Shi et al ., ) and heavy metals (Almond & Trombetta, ; Morcillo et al ., ). Besides pericardial edema, AF exposure caused the elongated heart morphology in zebrafish larvae in the treatment of 2.5, 5 and 10 μ m AF at 72 hpf in our study, which indicates that the developing heart is possibly a previous target for AF toxicity.…”

Section: Discussionmentioning

confidence: 97%

Developmental toxicity of auranofin in zebrafish embryos

Gao

Jiang

et al. 2016

J of Applied Toxicology

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Auranofin (AF) is used in clinic for the treatment of rheumatoid arthritis, repurposing of AF as an anticancer drug has just finished a phase I/II clinical trial, but the developmental toxicity of AF remains obscure. This study focused on its developmental toxicity by using zebrafish embryos. Zebrafish embryos were exposed to different concentrations (1, 2.5, 5, 10 μm) of AF from 2 h post-fertilization (hpf) to 72 hpf. At 72 hpf, two major developmental defects caused by AF were found, namely severe pericardial edema and hypopigmentation, when embryos were exposed to concentrations higher than 2.5 μm. Biochemical detection of oxidative stress enzyme combined with expressions of a series of genes related to oxidative stress, cardiac, metal stress and pigment formation were subsequently tested. The superoxide dismutase activity was decreased while malondialdehyde content was accumulated by AF treatment. The expression of oxidative stress-related genes (sod1, gpx1a, gst), pigment-related genes (mitfb, trp-1a) and one metal stress-related gene ctr1 were all decreased by AF exposure. The expressions of cardiac-related genes (amhc, vmhc) and one metal-related gene hsp70 were found to be significantly upregulated by AF exposure. These findings indicated the potential developmental toxicity of AF on zebrafish early development. Copyright © 2016 John Wiley & Sons, Ltd.

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“…These molecules facilitate the recruitment of leucocytes from the HK (functionally analogous to mammalian bone marrow) to the systemic circulation and ultimately to the inflammation site (Calder et al., 2013; Meseguer et al., 1995). Acidophilic granulocytes (functionally equivalent to mammalian neutrophils) seem to be the first cells to be recruited to the inflammation site, followed by monocytes/macrophages (Campos‐Sánchez & Esteban, 2021; Meseguer et al., 1994; Morcillo et al., 2015; Sepulcre et al., 2002). Interestingly, acidophilic granulocytes are responsible for the release of large amounts of ROS and proteolytic enzymes in order to kill microorganisms and to activate phagocytosis (Dovi et al., 2004; Shaw & Martin, 2009).…”

Section: Discussionmentioning

confidence: 99%

“…Interestingly, acidophilic granulocytes are responsible for the release of large amounts of ROS and proteolytic enzymes in order to kill microorganisms and to activate phagocytosis (Dovi et al., 2004; Shaw & Martin, 2009). Furthermore, peroxidases, lysozyme and proteases are mainly stored and released by acidophilic granulocytes (Meseguer et al., 1994; Morcillo et al., 2015; Sepulcre et al., 2002). In fact, the MPO and eosinophil peroxidase activities are considered a good marker of the activation of this type of leucocytes (Rodríguez et al., 2003).…”

Section: Discussionmentioning

confidence: 99%

Effects of subcutaneous injection of λ/κ‐carrageenin on the immune and liver antioxidant status of gilthead seabream (Sparusaurata)

Campos-Sánchez

Guardiola

Beltrán

et al. 2021

Journal of Fish Diseases

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This study investigated the acute inflammatory response induced by subcutaneous injection of carrageenin (1%) or phosphate‐buffered saline (control) in gilthead seabream (Sparus aurata). Skin mucus, serum, head kidney (HK) and liver were sampled at 1.5, 3 and 6 hr post‐injection (p.i.) to determine the immune and antioxidant status of this fish species. The skin mucus of the carrageenin group showed increased superoxide dismutase and peroxidase activities, lysozyme abundance, bactericidal activity against Vibrio anguillarum and Photobacterium damselae, and total immunoglobulins compared with those of the control group. However, the carrageenin‐injected fish sampled at 6 hr p.i. showed decreased protease activity in the skin mucus and peroxidase activity in the HK leucocytes compared with the control. Moreover, the carrageenin injection had no effects on the systemic immune system, but it reduced the liver catalase activities at both 3 and 6 hr in the carrageenin group relative to those in the control group. The expression levels of several proinflammatory and cell marker genes in the HK and liver were also determined. In the HK, the expression levels of interleukin‐1β and prostaglandin D synthase 1 were upregulated at 1.5 and 3 hr, respectively, in the carrageenin group compared with those in the control group. Contrarily, the expression of the NADPH oxidase subunit phox40 (an acidophilic granulocyte marker) in the carrageenin group at 6 hr was downregulated compared with that in the control group. These results suggested that subcutaneous injection of κ/λ‐carrageenin in gilthead seabream triggered an acute skin inflammation characterized by the rapid recruitment of acidophilic granulocytes and the release of humoral mediators into the skin mucus.

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Toxicological in vitro effects of heavy metals on gilthead seabream (Sparus aurata L.) head–kidney leucocytes

Cited by 33 publications

References 50 publications

New Insights into Cellular Impacts of Metals in Aquatic Animals

New Insights into Cellular Impacts of Metals in Aquatic Animals

Developmental toxicity of auranofin in zebrafish embryos

Effects of subcutaneous injection of λ/κ‐carrageenin on the immune and liver antioxidant status of gilthead seabream (Sparusaurata)

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