Toxicological findings about an anticancer fraction with casearins described by traditional and alternative techniques as support to the Brazilian Unified Health System (SUS)

Ferreira, Paulo Michel Pinheiro; Santos, Denise Barbosa; Silva, Jurandy do Nascimento; Goudinho, Amanda Freitas; Ramos, Carla Lorena Silva; Souza, Patrícia Canteri de; Almeida, Ricardo Sérgio; Moura, Diego Sousa; Oliveira, Rhaul; Grisólia, César Koppe; Cavalheiro, Alberto José; Melo‐Cavalcante, Ana Amélia de Carvalho; Ferreira, José Roberto de Oliveira; Filho, Manoel Odorico de Moraes; Pessoa, Cláudia

doi:10.1016/j.jep.2019.112004

Cited by 9 publications

(4 citation statements)

References 72 publications

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“…In the case of plant toxicity/poisoning, its harmful action must be proven experimentally. For humans, this experimental reproduction should be carried out in the same animal species, naturally affected, or related species (e.g., mice and rats), although different susceptibilities to the effects of toxic herbals among species are a common mark [ 60 , 61 ].…”

Section: Discussionmentioning

confidence: 99%

Chemotherapeutic and Safety Profile of a Fraction from Mimosa caesalpiniifolia Stem Bark

Ferreira

Drumond

Silva

et al. 2021

Journal of Oncology

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Mimosa caesalpiniifolia (Fabaceae) is used by Brazilian people to treat hypertension, bronchitis, and skin infections. Herein, we evaluated the antiproliferative action of the dichloromethane fraction from M. caesalpiniifolia (DFMC) stem bark on murine tumor cells and the in vivo toxicogenetic profile. Initially, the cytotoxic activity of DFMC on primary cultures of Sarcoma 180 (S180) cells by Alamar Blue, trypan, and cytokinesis block micronucleus (CBMN) assays was assessed after 72 h of exposure, followed by the treatment of S180-bearing Swiss mice for 7 days, physiological investigations, and DNA/chromosomal damage. DFMC and betulinic acid revealed similar in vitro antiproliferative action on S180 cells and induced a reduction in viable cells, induced a reduction in viable cells and caused the emergence of bridges, buds, and morphological features of apoptosis and necrosis. S180-transplanted mice treated with DFMC (50 and 100 mg/kg/day), a betulinic acid-rich dichloromethane, showed for the first time in vivo tumor growth reduction (64.8 and 80.0%) and poorer peri- and intratumor quantities of vessels. Such antiproliferative action was associated with detectible side effects (loss of weight, reduction of spleen, lymphocytopenia, and neutrophilia and increasing of GOT and micronucleus in bone marrow), but preclinical general anticancer properties of the DFMC were not threatened by toxicological effects, and these biomedical discoveries validate the ethnopharmacological reputation of Mimosa species as emerging phytotherapy sources of lead molecules.

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Section: Discussionmentioning

confidence: 99%

Chemotherapeutic and Safety Profile of a Fraction from Mimosa caesalpiniifolia Stem Bark

Ferreira

Drumond

Silva

et al. 2021

Journal of Oncology

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“…A Casearia sylvestris Swartz é uma planta que possui distribuições nas regiões tropicais e subtropicais do Brasil e também em outros países da América do Sul e Ásia (Ferreira et al, 2011). Conhecida popularmente como "Guaçatonga" ela está presente no uso popular e tradicional no Brasil (Ferreira et al, 2019;Ferreira et al, 2011). Esta planta compõe uma das 71 espécies que podem tratar as doenças com alta incidência no Brasil estando ela descrita na "Lista Nacional de Plantas Medicinais de Interesse do SUS" (RENISUS).…”

Section: Introductionunclassified

As propriedades antimicrobianas do extrato glicólico de Casearia sylvestris Sw. em biofilme monotípico de cepas de Candida albicans e Candida glabrata

Assis

Santos

Silva

et al. 2022

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Objetivos: A resistência dos microrganismos perante o uso indevido dos fármacos é atualmente um dos problemas de saúde pública, tornando-se necessárias pesquisas sobre compostos naturais com atividade antimicrobiana, como a Casearia sylvestris. Este estudo objetivou avaliar a atividade antimicrobiana do extrato glicólico de Casearia sylvestris sobre cepas-padrão de Candida albicans (ATCC 18804) e Candida glabrata (ATCC 9030) em cultura planctônica, verificando a concentração inibitória mínima e concentração microbicida mínima (CIM e CMM) e também sua ação sobre biofilmes. Metodologia: O extrato glicólico de C. sylvestris, de origem comercial, foi obtido na concentração de 200 mg/ml (20%) eluídos em 80% de propilenoglicol. Os valores da CIM e CMM foram determinados pelo método de microdiluição em caldo, segundo Clinical and Laboratory Standards Institute (CLSI), normas M27-A2, com modificações. Logo, foram iniciados os testes em biofilmes monotípicos de 48h, com troca de caldo a cada 24h, destes micro-organismos. Após submetido aos tratamentos o biofilme foi preparado para o teste metabólico de MTT. Resultados: Candida albicans apresentou CMM em 50 mg/mL, em Candida glabrata não observou-se CIM e CMM. No teste de MTT os resultados mostraram efetividade da concentração de 25 mg/mL sobre Candida glabrata, com redução de ≥ 29%, em Candida albicans não observou-se resultados significativos nas concentrações analisadas. Conclusão: O extrato apresentou efeito antimicrobiano contra os gêneros testados. Salienta-se também a importância de testes in vitro e in vivo que corroborem os resultados apresentados, com finalidade de aumentar o embasamento da aplicação do extrato.

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“…It has a huge pharmacological and cytotoxic arsenal, anti-inflammatory, antiplasmodial, and anti-ulcer properties [24]. C. sylvestris (“guaçatonga”) is part of popular/traditional use in Brazil [24, 25]. This plant is cited in the “National List of Medicinal Plants of Interest to SUS” (RENISUS), which contains 71 species that could treat the diseases with a high incidence in Brazil [25].…”

Section: Introductionmentioning

confidence: 99%

“…C. sylvestris (“guaçatonga”) is part of popular/traditional use in Brazil [24, 25]. This plant is cited in the “National List of Medicinal Plants of Interest to SUS” (RENISUS), which contains 71 species that could treat the diseases with a high incidence in Brazil [25]. Indigenous tribes use the macerated bark of C. sylvestris to treat gastrointestinal disorders (i.e., diarrhea), leprosy, snake bites, and to heal wounds [24], while decocted bark is used as an anti-inflammatory [26], and for snake bite where the bark is infused for on-site application [27].…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial and antibiofilm activities of Casearia sylvestris extracts from distinct Brazilian biomes against Streptococcus mutans and Candida albicans

Ribeiro

Fratucelli

Bueno

et al. 2019

BMC Complement Altern Med

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BackgroundDental caries is a biofilm-diet-dependent worldwide public health problem, and approaches against microorganisms in cariogenic biofilms are necessary.MethodsThe antimicrobial and antibiofilm activities of 12 Casearia sylvestris extracts (0.50 mg/mL) from different Brazilian biomes (Atlantic Forest, Cerrado, Caatinga, Pampa, and Pantanal) and varieties (sylvestris, lingua, and intermediate) were tested against two species found in cariogenic biofilms (Streptococcus mutans and Candida albicans). The extracts effective against S. mutans were used to evaluate the "adhesion strength" of this bacterium to the salivary pellicle and initial glucan matrix and the S. mutans-GtfB activity. Also, the antimicrobial activity against S. mutans of three fractions (methanol, ethyl acetate, and hexane; 0.25 mg/mL) from the extracts was evaluated.ResultsThree extracts from the Atlantic Forest variety sylvestris (FLO/SC, GUA/CE, PRE/SP) reduced ≥50% (> 3 logs) S. mutans viable population (p < 0.0001 vs. vehicle), while two extracts from the same biome and variety (PAC/CE, PRE/SP) decreased ≥50% of the viable counts of C. albicans (p < 0.0001 vs. vehicle). For S. mutans biofilms, three extracts (GUA/CE, PAC/CE, PRE/SP) reduced the biomass by ≥91% (p > 0.0001 vs. vehicle) and 100% of the microbial population (p < 0.0001 vs. vehicle). However, for the fungal biofilm, two extracts (PAC/CE, PRE/SP) reduced the viable counts by ≥52% (p < 0.0001 vs. vehicle), but none reduced biomass. The extracts with higher antimicrobial and antibiofilm activities presented higher content of clerodane-type diterpenes and lower content of glycosylated flavonoids than the less active extracts. The extracts had no effect on the removal of cells adhered to the pellicle (p > 0.05 vs. vehicle) while promoted the detachment of a larger number of S. mutans cells from GtfB-glucan matrix (p < 0.0031 vs. vehicle), and FLO/SC, GUA/CE and PRE/SP reduced the quantity of glucans (p < 0.0136 vs. vehicle). Only the ethyl acetate fractions reduced the microbial population of S. mutans (p < 0.0001 vs. vehicle), except for one (PAC/CE). Among the ethyl acetate fractions, three from var. lingua (two from Cerrado, and one from Cerrado/Caatinga) reduced ≥83% of the microbial population.ConclusionsC. sylvestris extracts from Atlantic Forest var. sylvestris and ethyl acetate fractions from Cerrado and Cerrado/Caatinga var. lingua may be used as a strategy against cariogenic microorganisms.

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Toxicological findings about an anticancer fraction with casearins described by traditional and alternative techniques as support to the Brazilian Unified Health System (SUS)

Cited by 9 publications

References 72 publications

Chemotherapeutic and Safety Profile of a Fraction from Mimosa caesalpiniifolia Stem Bark

Chemotherapeutic and Safety Profile of a Fraction from Mimosa caesalpiniifolia Stem Bark

As propriedades antimicrobianas do extrato glicólico de Casearia sylvestris Sw. em biofilme monotípico de cepas de Candida albicans e Candida glabrata

Antimicrobial and antibiofilm activities of Casearia sylvestris extracts from distinct Brazilian biomes against Streptococcus mutans and Candida albicans

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