Toxicological evaluation of neoagarooligosaccharides prepared by enzymatic hydrolysis of agar

Hong, Sang Jeen; Lee, Je-Hyeon; Kim, Eun Joo; Yang, Hea Jung; Park, Jae-Seon; Hong, Soon-Kwang

doi:10.1016/j.yrtph.2017.08.001

Cited by 32 publications

(22 citation statements)

References 21 publications

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“…Studies have shown that neoagarotetraose exhibits various biological activities, and because of its nontoxic properties, neoagarotetraose can be used in cosmetic, health food, and pharmaceutical industries (Jang et al 2009 ; Hong et al 2017b ). For example, Jang et al ( 2009 ) pointed out that 0.1 μg/mL neoagarotetraose could reduce the melanin content in murine melanoma B16F10 cells and could inhibit tyrosinase activity in B16F10 cells and mushroom tyrosinase activity in vitro; thus, it is a potential skin-whitening agent.…”

Section: Discussionmentioning

confidence: 99%

Molecular cloning, expression, and functional characterization of the β-agarase AgaB-4 from Paenibacillus agarexedens

et al. 2018

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In this study, a β-agarase gene, agaB-4, was isolated for the first time from the agar-degrading bacterium Paenibacillus agarexedens BCRC 17346 by using next-generation sequencing. agaB-4 consists of 2652 bp and encodes an 883-amino acid protein with an 18-amino acid signal peptide. agaB-4 without the signal peptide DNA was cloned and expressed in Escherichia coli BL21(DE3). His-tagged recombinant AgaB-4 (rAgaB-4) was purified from the soluble fraction of E. coli cell lysate through immobilized metal ion affinity chromatography. The optimal temperature and pH of rAgaB-4 were 55 °C and 6.0, respectively. The results of a substrate specificity test showed that rAgaB-4 could degrade agar, high-melting point agarose, and low-melting point agarose. The Vmax and Km of rAgaB-4 for low-melting point agarose were 183.45 U/mg and 3.60 mg/mL versus 874.61 U/mg and 9.29 mg/mL for high-melting point agarose, respectively. The main products of agar and agarose hydrolysis by rAgaB-4 were confirmed to be neoagarotetraose. Purified rAgaB-4 can be used in the recovery of DNA from agarose gels and has potential application in agar degradation for the production of neoagarotetraose.Electronic supplementary materialThe online version of this article (10.1186/s13568-018-0581-8) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Molecular cloning, expression, and functional characterization of the β-agarase AgaB-4 from Paenibacillus agarexedens

et al. 2018

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“…Agar, an important marine polysaccharide extracted from red algae, is a compound polysaccharide made of repeating structures of β‐1,4‐D‐galactopyranosy and l‐α‐1,3‐L‐galactopyranose (Hu et al ., ; Enoki et al ., ; Hong et al ., ). Agar is widely used as thickening agent, coagulant and emulsifier in biochemistry, food, cosmetics and medicine due to its high viscosity and good gelling ability (Wang et al ., ; Li et al ., ).…”

Section: Introductionmentioning

confidence: 97%

“…Agar oligosaccharide has good water solubility and is easily absorbed by the body, and the molecule containing a large number of hydroxyl and carboxyl groups (Kazłowski et al ., ; Hong et al ., ) may play a stable coordination effect on metal ions. So, this study focused on preparation of the AOS–iron (III) complex using AOS and ferric chloride, and investigation of its physicochemical and structural characteristics.…”

Section: Introductionmentioning

confidence: 99%

Preparation and characterisation of a novel agar oligosaccharide–iron (III) complex

Teng

et al. 2018

Int J of Food Sci Tech

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This study aimed to synthesise a complex of agar oligosaccharides (AOS) with ferric iron and to evaluate its physicochemical and structural characteristics. Based on single factor, Plackett-Burman and response surface optimal experiments of three factors at three levels, the optimised chelate conditions of the AOSiron (III) complex were mass ratio of AOS to iron ions of 4.5:1 g:g, reaction temperature of 74°C and reaction time of 60 min. Under these conditions, the iron content and total sugar content were 14.03 AE 0.42% and 66.07 AE 0.96%, respectively. The physicochemical analysis showed that AOS-iron (III) was soluble and steady at physiological pH, and it was easily reduced to iron (II). The structural characteristics of AOS and AOS-iron (III) were analysed by Raman spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction and Nuclear magnetic resonance, and results showed that the iron (III) ions were chelated to AOS by -OH groups and -COOH groups and widely dispersed on the AOS backbone. Therefore, AOS-iron (III) could be a candidate for iron supplementation to treat iron deficiency anaemia.

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“…AOS has specic physiological functions, such as antioxidant, 19 prebiotic, 20 anti-inammatory, 21 and antimicrobial activity, glucosidase inhibition and melanin biosynthesis. 22 In particular, AOS has high water solubility, is easily absorbed by the body, and contains a large number of free hydroxyl groups, 23,24 which may coordinate with metal ions. Hence, AOS is a suitable choice as a ligand for chelating iron.…”

Section: Introductionmentioning

confidence: 99%

Effectiveness of AOS–iron on iron deficiency anemia in rats

Huang

Liu

et al. 2019

RSC Adv.

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Toxicological evaluation of neoagarooligosaccharides prepared by enzymatic hydrolysis of agar

Cited by 32 publications

References 21 publications

Molecular cloning, expression, and functional characterization of the β-agarase AgaB-4 from Paenibacillus agarexedens

Molecular cloning, expression, and functional characterization of the β-agarase AgaB-4 from Paenibacillus agarexedens

Preparation and characterisation of a novel agar oligosaccharide–iron (III) complex

Effectiveness of AOS–iron on iron deficiency anemia in rats

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