Toxicological evaluation of hydroethanolic extract of Helicteres sacarolha A. St.- Hil. et al.

Balogun, Sikiru Olaitan; Silva, Iberê Ferreira da; Colodel, Edson Moleta; Oliveira, Ruberlei Godinho de; Ascêncio, Sérgio Donizeti; Martins, Domingos Tabajara de Oliveira

doi:10.1016/j.jep.2014.09.013

Cited by 49 publications

(26 citation statements)

References 27 publications

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“…Relative organ weight is a reliable indicator which can be used to measure the degree of toxicity of any drugs or chemicals [38]. Our study demonstrated that in male rats, after administration of extract at different doses all organs increased mostly significantly except stomach which showed a decrease in weight, whereas in female rats only stomach and ovary showed decrease in their weight.…”

Section: Discussionmentioning

confidence: 56%

Acute and sub-acute (30-day) toxicity studies of Aegialitis rotundifolia Roxb., leaves extract in Wistar rats: safety assessment of a rare mangrove traditionally utilized as pain antidote

2019

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Acute and sub-acute (30-day) toxicity studies of Aegialitis rotundifolia Roxb., leaves extract in Wistar rats: safety assessment of a rare mangrove traditionally utilized as pain antidote Abstract Background: Aegialitis rotundifolia Roxb., (Plumbaginaceae) is a small mangrove tree or shrub traditionally used by the local healers of mangroves for curing pain and inflammation. However, there are no scientific reports of its toxicological properties which would guarantee the safety of its folkloric usage as a potent pain reliever. Therefore, the present study deals in acute and sub-acute (30-day) toxicity studies of Aegialitis rotundifolia ethanolic leaves extract (ARELE) on male and female Wistar rats administered through oral gavage.Methods: Acute toxicity study was conducted at a single oral dose of 1000, 1500, and 2000 mg/kg, body weight (b.wt.) for 14 days with a special emphasis on the first four hours after drug administration to find out any mortality and morbidity. In sub-acute toxicity, the extract at the doses of 125, 250 and 500 mg/kg, b.wt., was administered orally for 30 days. Important parameters such as general behaviour, body and organ weight, urinalysis, haematological and biochemical profile, organ macroscopy and microscopy were conducted. Organ sample of liver, kidney, pancreas, heart, lungs, and stomach were taken from both male and female rats, whereas the sample of testis and ovary was taken from male and female rats respectively for organ necropsy and histopathological studies. Neurobehavioral toxicity was conducted by performing a functional observational battery (FOB) and locomotor activity on the initial and final week of the 30-day study period. Results:No mortality or any major signs of morbidity was recorded for acute toxicity except for the limit dose (2000 mg/kg, b.wt.) which produced a slight short-term sedative effect. In sub-acute toxicity, no major alteration was observed in the evaluated parameters. However, few minor changes were recorded for high dose (500 mg/kg, b.wt.) group. Conclusion:The results of the present study showed that oral administration of Aegialitis rotundifolia ethanolic leaves extract did not produce any severe toxic effects in both acute and sub-acute studies in Wistar rats. Therefore, usage of an appropriate amount of Aegialitis rotundifolia leaves extract preferably at low doses for its traditional use should be considered safe.

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Section: Discussionmentioning

confidence: 56%

Acute and sub-acute (30-day) toxicity studies of Aegialitis rotundifolia Roxb., leaves extract in Wistar rats: safety assessment of a rare mangrove traditionally utilized as pain antidote

2019

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“…The antiurolithiatic property of this formulation makes it imperative to be used for a long period of time; hence there is a dire need to establish its safety profile. Acute toxicity is usually an initial study performed: to serve as the basis for classification and labeling, to provide initial information on the mode of toxic action of a substance, to help arrive at a dose of a new compound, to aid in the identification of the further range of doses in animal studies and also for calculating the therapeutic index of drugs and chemicals 31,33 . Administration of hydroalcoholic extract of majoon hajrul yahood in rats does not cause death.…”

Section: Discussionmentioning

confidence: 99%

“…The functional capacity of the kidney was evaluated via the determination of the serum urea and creatinine levels 1 . Clinical biochemistry tests are used in toxicology studies to generate data that are crucial for evaluating altered organ function or major toxic effects in tissues in experimental animals 33 .…”

Section: Discussionmentioning

confidence: 99%

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2020

IJPSR

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“…Mass of the organs is often used as a sensitive indicator for evaluating the toxic impact of different substances, including medical (Balogun et al, 2014;Varcholyak & Gutyi, 2019). Relative mass of the organs of animals following the diet with excessive content of fat changed both under the influence of alcohol and alcohol tincture of Aralia.…”

Section: Discussionmentioning

confidence: 99%

Effect of alcohol tincture of Aralia elata on the organism of rats and their gut microbiota against the background of excessive fat diet

Brygadyrenko

Lieshchova

Bilan

et al. 2019

Regul. Mech. Biosyst.

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Pharmacological effects of the medicinal plant Aralia elata (Miq.) Seem. 1868 (Araliaceae) are related to presence of over 150 secondary metabolites, including flavonoids, sterols, polysaccharides, terpenoid saponins and terpenoid acids, though the main biologically active substances of A. elata are saponins and flavonoids. Some clinical tests and experimental studies have proved the influence of A. elata on the organism through increase in physical work capacity, exerting an anti-stress effect against a broad range of harmful factors, including stress from cold, immobilization, ultraviolet radiation and low pressure. The literature also reports the anti-ulcer, anti-secretory, antifungal, anti-tumour, and antimicrobial activity of preparations from this plant. In our laboratory experiment, we determined that ethanolic tincture of A. elata has no effect on the intensity of growth of body weight of young rats against the background of excessive content of fat in their diet. Excessive fat-feeding of male rats leads mostly to disorders in the functioning of the liver and development of steatosis. This was accompanied by reduction in relative mass of the liver, increase in activity of hepatic enzymes, dysproteinemia, increase in the level of bilirubin and decrease in the level of urea. Additional use of 0.1% ethanol led to impaired functioning of the kidneys, reduction of their relative mass, signs of dehydration, increase in the level of creatinine and total calcium in the blood. Use of ethanolic tincture of A. elata mitigates negative excess of fat, is accompanied by normalization of indices of mass of the organs, less notable dysproteinemia, impairment in the level of creatinine, glucose, urea, cholesterol, bilirubin and total calcium. Ethanolic tincture of A. elata has a low immunosuppressive action, against the background of a high fat diet it leads to increase in the amount of typical Escherichia сoli, decrease in Еnterococcus spp. and Enterobacter spp., significant decrease and in high concentrations (0.1% ethanolic tincture of A. elata) elimination of bacteria of Clostridium and Klebsiella genera, and also various yeast fungi in the intestine. In the examined male rats, against the background of excess of fat in the diet, no serious changes in the composition of the normal gut microbiota (Bifidobacterium spp., Lactobacillus spp., Proteus spp., Staphylococcus spp., Candida spp.) was observed, nor were any lactose-negative enterobacteria (Citrobacter genus) found. Perspectives of further research include determining histological, histochemical and immune-histological changes in the organs of laboratory animals under the effect of ethanolic tincture of A. elata following excessive accumulation of fat.

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Toxicological evaluation of hydroethanolic extract of Helicteres sacarolha A. St.- Hil. et al.

Abstract: Our results confirmed that HEHs have a broad safety margin for therapeutic use.

Cited by 49 publications

References 27 publications

Acute and sub-acute (30-day) toxicity studies of Aegialitis rotundifolia Roxb., leaves extract in Wistar rats: safety assessment of a rare mangrove traditionally utilized as pain antidote

Acute and sub-acute (30-day) toxicity studies of Aegialitis rotundifolia Roxb., leaves extract in Wistar rats: safety assessment of a rare mangrove traditionally utilized as pain antidote

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Effect of alcohol tincture of Aralia elata on the organism of rats and their gut microbiota against the background of excessive fat diet

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