Toxicological evaluation of fucoidan from Undaria pinnatifidain vitro and in vivo

Chung, Hyuck Jin; Jeun, Jungae; Houng, Soung Jin; Jun, Hee-Jin; Kweon, Dong Keon; Lee, Sung Joon

doi:10.1002/ptr.3138

Cited by 54 publications

(45 citation statements)

References 30 publications

(32 reference statements)

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“…In spite of several studies on the biological activities of fucans, detailed studies on the toxicology of fucans from different sources are limited. Few studies have shown that some algal fucans are not toxic compounds (Li et al, 2005;Gideon & Rengasamy, 2008;Zaragozá et al, 2008;Chung et al, 2010). However, each new fucan purified from a marine alga is a new compound with unique structures and, consequently, with potential pharmacological and toxicological properties.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of acute and subchronic toxicity of a non-anticoagulant, but antithrombotic algal heterofucan from the Spatoglossum schröederi in Wistar rats

Almeida-Lima¹,

Dantas-Santos²,

Gomes³

et al. 2011

Rev. bras. farmacogn.

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Abstract:Fucan is a term used to denominate a family of sulfated polysaccharides rich in L-fucose. The brown alga Spatoglossum schröederi, Dictyotaceae, synthesizes three heterofucans named A, B, and C. Fucan A is a non-anticoagulant heterofucan which possesses potent antithrombotic (in vivo) and antiproliferative (in vitro) activities. However, its toxicity in vivo has not been determined. The present study examined the acute and subchronic toxicity of the fucan A in Wistar rats after subcutaneous administration. After that, the animals were killed and examined. The results showed in the acute study that fucan A did not cause general adverse effects and mortality in the concentrations 0, 20, 100, 1000, and 2000 µg/g body weight per rat for seven days. Regarding the subchronic study, the data showed that the fucan A did not cause any change in hematological and biochemistry parameters, as well as in the morphology, and in the size of the rat's organs analyzed at a concentration of 20 µg/g body weight per rat during a 62-day period. In conclusion, this study indicates this heterofucan is a compound with potential pharmacological value that has no toxicity in vivo.

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Section: Introductionmentioning

confidence: 99%

Evaluation of acute and subchronic toxicity of a non-anticoagulant, but antithrombotic algal heterofucan from the Spatoglossum schröederi in Wistar rats

Almeida-Lima¹,

Dantas-Santos²,

Gomes³

et al. 2011

Rev. bras. farmacogn.

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“…The fiber in seaweed species amounts to 30% of dry weight and includes AF, which have chemical structures different from those of the dietary fibers found in vegetables and grains. In recent toxicological reports, fucoidan derived from U. pinnatifid and Laminaria japonica was found to be safe in animal models at very high levels of intake (32,33). In addition, wakame is a useful source of sodium, potassium, and magnesium (34).…”

Section: Discussionmentioning

confidence: 99%

Wakame (Undaria pinnatifida ) modulates hyperphosphatemia in a rat model of chronic renal failure

et al. 2015

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AnimalsNormal Wistar rats and 5/6 nephrectomized (5/6NX) rats were purchased from Shimizu Laboratory Supplies Co., Ltd. (Kyoto, Japan). The (5/6NX) rats were established by general method (22). The 5/6 nephrectomy was performed at the age of 7 weeks under anesthesia with sodium pentobarbital (50 mg/kg body weight, i.p.). The operation began with ablation of approximately the entire left kidney, and then 2/3 of the right kidney was removed by ligation of the renal artery, vein, and ureter 1 week later. After acclimatization (2 weeks), the 5/6NX rats were divided into two groups by the follow-up period. Rats were maintained under pathogen-free conditions and handled in accordance with the Guidelines for Animal Experimentation of Doshisha Women's College of Liberal Arts. Abstract : In chronic renal failure, inorganic phosphate (Pi) retention speeds up the progression to end-stage renal disease. The current therapy for hyperphosphatemia in patients with chronic renal failure consists of dietary Pi restriction combined with administration of Pi binders, but each therapy has practical problems. Thus, the discovery of foods or nutrients that inhibit Pi absorption may be useful for the treatment of hyperphosphatemia. In the present study, we investigated whether wakame (Undaria pinnatifida) is a useful food for the prevention of hyperphosphatemia in a rat model of renal failure. Feeding a diet containing 5% % wakame significantly decreased plasma and urinary Pi levels and increased the amount of fecal Pi. In addition, wakame significantly reduced plasma blood urea nitrogen and plasma Pi levels in 5/6 nephrectomized rats fed a high-Pi diet. Biochemical analyses showed that the reduction of intestinal Pi absorption is the main reason for the decrease in plasma Pi levels in rats fed a diet containing wakame. In addition, feeding alginic acid and fucoidan, major components of wakame fiber, was effective in reducing plasma Pi levels in normal rats. Finally, we concluded that wakame may be a useful food for the prevention of hyperphosphatemia in rodents. J. Med. Invest. 62 : 68-74, February, 2015 ORIGINAL Wakame

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“…The vegetation-to-background uptake ratio was 5.2 (2.7-9.1). On autoradiography, the 99m Tc-fucoidan uptake ratio was 17 (14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32). Coregistration of autoradiography with histologic analysis performed after routine staining of tissue slices showed a clear delineation of the tracer uptake within the vegetations (Supplemental Fig.…”

Section: Endocarditic Vegetationsmentioning

confidence: 97%

“…First, its toxicity has been investigated in recent years for the development of medicines, cosmetics, and food supplements, with no severe adverse effect reported in animals or humans (Material Safety Data, Algues & Mer) (31,32). We have already tested a high dose of fucoidan (5 mg/kg/d intramuscularly during 2 wk) in experimental therapeutics in rats without detectable side effects (33).…”

Section: P-selectin-targeted Imaging Agentsmentioning

confidence: 99%

Radiolabeled Fucoidan as a P-Selectin Targeting Agent for In Vivo Imaging of Platelet-Rich Thrombus and Endothelial Activation

Rouzet

Bachelet-Violette

Alsac³

et al. 2011

J Nucl Med

116

107

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P-selectin expression is involved in the pathophysiology of biologically active arterial thrombus and endothelial activation after a transient ischemic event. Fucoidan is a polysaccharidic ligand of P-selectin, with a nanomolar affinity. In the present study, we propose a new approach of P-selectin molecular imaging based on radiolabeled fucoidan. Methods: Two kinds of experimental models were selected to evaluate the ability of radiolabeled fucoidan to detect P-selectin expression: plateletrich arterial thrombi (vegetations of infective endocarditis and arterial mural thrombus) and myocardial ischemia-reperfusion. These 2 settings were chosen because they were clinically relevant, and both were associated with an important overexpression of platelet and endothelial P-selectin, respectively. Results: 99m Tc-fucoidan SPECT was able to detect the presence of platelet-rich arterial thrombi in all animals, with a median target-to-background ratio of 5.2 in vegetations of endocarditis and 3.6 in mural aneurysmal thrombus, and to detect a persistent endothelial activation at 2 h after reperfusion. In this latter model, the magnitude of the signal was correlated with the extent of myocardium that underwent transient ischemia. The sensitivity of selectivity of the uptake and retention of 99m Tc-fucoidan in both settings was excellent. Conclusion: This study supports 99m Tc-fucoidan as a relevant imaging agent for in vivo detection of biologic activities associated with P-selectin overexpression, such as arterial thrombus and ischemic memory. Given the reported wide availability at a low cost, and its low toxicity, fucoidan seems to overcome some of the limitations of previous P-selectin-targeted imaging agents.Key Words: P-selectin; fucoidan; thrombus; ischemia; imaging J Nucl Med 2011; 52:1433-1440 DOI: 10.2967/jnumed.110.085852 P-sel ectin is an adhesion molecule expressed at the surface of endothelial cells and platelets on activation. It mediates leukocyte rolling on activated endothelium (1) and leukocyte trapping on platelet aggregates (2), through the interaction with its counterreceptor P-selectin glycoprotein ligand 1. In the cardiovascular field, P-selectin expression is involved in the pathophysiology of 2 settings of prominent clinical relevance: the renewal and growth of biologically active (at risk) arterial thrombus (2) and endothelial activation after an acute transient myocardial ischemic event referred to as ischemic memory (3). Activated platelets expose granular P-selectin on their membrane surface, and myocardial injury is associated with activation of endothelial cells resulting in Weibel-Palade bodies exocytosis and overexpression of adhesion molecules (1,4), which persists after ischemia has resolved. Therefore, P-selectin promotes platelet, endothelium, and leukocyte interactions, whatever the cardiovascular events, representing an important molecular target in acute and in chronic cardiovascular diseases.Consequently, many efforts have been made to develop imaging agents targeting P-selectin (5...

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Toxicological evaluation of fucoidan from Undaria pinnatifidain vitro and in vivo

Cited by 54 publications

References 30 publications

Evaluation of acute and subchronic toxicity of a non-anticoagulant, but antithrombotic algal heterofucan from the Spatoglossum schröederi in Wistar rats

Evaluation of acute and subchronic toxicity of a non-anticoagulant, but antithrombotic algal heterofucan from the Spatoglossum schröederi in Wistar rats

Wakame (<i>Undaria pinnatifida </i>) modulates hyperphosphatemia in a rat model of chronic renal failure

Radiolabeled Fucoidan as a P-Selectin Targeting Agent for In Vivo Imaging of Platelet-Rich Thrombus and Endothelial Activation

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