Toxicological, Electrophysiological, and Molecular Characterisation of Knockdown Resistance to Pyrethroid Insecticides in the Diamondback Moth,Plutella xylostella(L.)

Schuler, T. H.; Martínez‐Torres, David; Thompson, Andrew; Denholm, I.; Devonshire, A. L.; Duce, I.R.; Williamson, Martin S.

doi:10.1006/pest.1998.2320

Cited by 143 publications

(128 citation statements)

References 43 publications

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“…The T929I mutation has been identified in pyrethroid-resistant diamondback moth, Plutella xylostella, and shown to be the causative mutation for kdr-like resistance in that species (Schuler et al, 1998). A previous study of the impact of the T929I mutation on the permethrin sensitivity of Para/tipE sodium channels showed that this mutation conferred a 13-fold decrease in sensitivity (Vais et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

“…All three mutations are found en bloc as a haplotype in permethrin-resistant field populations of head louse . T917I, corresponding to T929I in the house fly, has been functionally validated as a kdr-type mutation in the diamondback moth, Plutella xylostella (Schuler et al, 1998). It is not yet clear, however, whether the two novel mutations (M815I and L920F) found in head lice have functional significance in pyrethroid resistance.…”

Section: Introductionmentioning

confidence: 99%

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Three mutations identified in the voltage-sensitive sodium channel α-subunit gene of permethrin-resistant human head lice reduce the permethrin sensitivity of house fly Vssc1 sodium channels expressed in Xenopus oocytes

SupYoon

Symington

Lee

et al. 2008

Insect Biochemistry and Molecular Biology

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Point mutations in the para-orthologous sodium channel α-subunit of the head louse (M815I, T917I and L920F) are associated with permethrin-and DDT-resistance. These mutations were inserted in all combinations using site-directed mutagenesis at the corresponding amino acid sequence positions (M827I, T929I and L932F) of the house fly para-orthologous voltage-sensitive sodium channel α-subunit (Vssc1 WT ) gene and heterologously co-expressed with the sodium channel auxiliary subunit of house fly (Vsscβ) in Xenopus oocytes. The double mutant possessing M827I and T929I (Vssc1 MITI /Vsscβ) caused a ~4.0 mV hyperpolarizing shift and the triple mutant, Vssc1 MITILF / Vsscβ, caused a ~3.2 mV depolarizing shift in the voltage dependence of activation curves. Vssc1 MITI /Vsscβ, Vssc1 TILF /Vsscβ and Vssc1 MITILF /Vsscβ caused depolarizing shifts (~6.6, ~7.6 and ~8.8 mV, respectively) in the voltage dependence of steady-state inactivation curves. The M827I and L932F mutations reduced permethrin sensitivity when expressed alone but the T929I mutation, either alone or in combination, virtually abolished permethrin sensitivity. Thus, the T929I mutation is the principal cause of permethrin resistance in head lice. Comparison of the expression rates of channels containing single, double and triple mutations with that of Vssc1 WT /Vsscβ channels indicates that the M827I mutation may play a role in rescuing the decreased expression of channels containing T929I.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Three mutations identified in the voltage-sensitive sodium channel α-subunit gene of permethrin-resistant human head lice reduce the permethrin sensitivity of house fly Vssc1 sodium channels expressed in Xenopus oocytes

SupYoon

Symington

Lee

et al. 2008

Insect Biochemistry and Molecular Biology

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“…CpBV-S16 처리구의 경우 vankyrin의 발현이 나오지 않았고 Bt 감수성 증가도 없는 것으로 확인되었다. Mortality (%) at 5 DAT (Schuler et al, 1998). 이때 L1014F는 kdr (knockdown resistance) 저항성이고 이 두 점돌연변이가 결부되면 skdr (super-kdr) 저항성으로 나타나게 된다 (Williamson et al, 1996).…”

Section: Vankyrin 유전자의 잠시발현 유도 및 Bt 감수성 증가unclassified

An Integrated Biological Control Using an Endoparasitoid Wasp (Cotesia plutellae) and a Microbial Insecticide (Bacillus thuringiensis) against the Diamondback Moth, Plutella xylostella

Kim¹,

Kim²,

Park³

et al. 2013

Korean journal of applied entomology

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All tested Korean populations of the diamondback moth, Plutella xylostella, are known to be resistant especially against pyrethroid insecticides by mutation in its molecular target, para-sodium channel. Moreover, P. xylostella is able to develop resistance against most commercial insecticides. This study was performed to develop an efficient control technique against P. xylostella by a combined treatment of an endoparasitoid wasp, Cotesia plutellae, and a microbial insecticide, Bacillus thurigiensis. To investigate any parasitism preference of C. plutellae against susceptible and resistant P. xylostella, five different populations of P. xylostella were compared in insecticide susceptibilities and parasitism by C. plutellae. These five P. xylostella populations showed a significant variation against three commercial insecticides including pyrethroid, organophosphate, neonicotinoid, and insect growth regulator. However, there were no significant differences among five P. xylostella populations in their parasitic rates by C. plutellae. Moreover, parasitized larvae of P. xylostella showed significantly higher susceptibility to B. thuringiensis. As an immunosuppressive agent, viral ankyrin genes (vankyrins) encoded in C. plutellae were transiently expressed in nonparasitized larvae. Expression of vankyrins significantly enhanced the efficacy of B. thuringiensis against the third instar larvae of P. xylostella. Thus an immunosuppression induced by C. plutellae enhanced the insecticidal efficacy of B. thuringiensis. These results suggest that a combined treatment of C. plutellae and B. thuringiensis may effectively control the insecticide-resistant populations of P. xylostella.

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“…Accordingly, these sites have proved to be important for the interaction between Na V , in the D. melanogaster sodium channel and pyrethroids or DDT (Usherwood et al, 2007). Substitutions in site 929 are also involved in enhanced pyrethroid resistance, as is the case with the Lepidoptera Plutella xylostella mutation Thr929Ile, detected in association with Leu1014Phe (Schuler et al, 1998). However, in the maize weevil Sitophilus zeamais, the Thr929Ile was found alone (Araujo et al, 2011).…”

Section: The Kdr Mutationmentioning

confidence: 99%

The Pyrethroid Knockdown Resistance

Martins¹,

Valle²

2012

Insecticides - Basic and Other Applications

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Toxicological, Electrophysiological, and Molecular Characterisation of Knockdown Resistance to Pyrethroid Insecticides in the Diamondback Moth,Plutella xylostella(L.)

Cited by 143 publications

References 43 publications

Three mutations identified in the voltage-sensitive sodium channel α-subunit gene of permethrin-resistant human head lice reduce the permethrin sensitivity of house fly Vssc1 sodium channels expressed in Xenopus oocytes

Three mutations identified in the voltage-sensitive sodium channel α-subunit gene of permethrin-resistant human head lice reduce the permethrin sensitivity of house fly Vssc1 sodium channels expressed in Xenopus oocytes

An Integrated Biological Control Using an Endoparasitoid Wasp (Cotesia plutellae) and a Microbial Insecticide (Bacillus thuringiensis) against the Diamondback Moth, Plutella xylostella

The Pyrethroid Knockdown Resistance

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