Toxicological effects of silver nanoparticles and cadmium chloride in macrophage cell line (RAW 264.7): An in vitro approach

Gliński, A.; Souza, Tugstênio Lima de; Luz, Jessica Zablocki da; Bezerra, Arandi Ginane; Oliveira, Carolina Camargo de; Ribeiro, Ciro Alberto de Oliveira; Neto, Francisco Filipak

doi:10.1016/j.jtemb.2021.126854

Cited by 5 publications

(2 citation statements)

References 67 publications

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“…There was a significant reduction in the viability of J774 A.1 in the highest concentrations of OCNT TEPA tested, indicating possible cytotoxic effects dependent on mitochondrial enzymes, since there was a decrease in MTT metabolism in cells. Neutral red has the same purpose, but is able to be absorbed by living cells and concentrate inside lysosomes for quantification of living cells [20,21]. These data corroborate other experiments carried out with MWCNTs using lines of murine alveolar macrophages (MH-S) and murine peritoneal macrophages (raw264.7), which showed decreased cell viability in the presence of higher concentrations of MWCNTs [22].…”

Section: Discussionsupporting

confidence: 86%

Cytotoxic Effects Caused by Functionalized Carbon Nanotube in Murine Macrophages.

Godoy

Rodolpho

Fragelli

et al. 2022

Cell Physiol Biochem

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BACKGROUND/AIMS: The development of new nanomaterials has been growing in recent decades to bring benefits in several areas, especially carbon-based nanoparticles, which have unique physical-chemical properties and allow to take on several applications. Consequently, the use of new nanomaterials without previous toxicological studies raises concern about possible harmful health effects. The aim of this study was to investigate the cytotoxic profile of a new multi-walled carbon nanotube (MWCNT) functionalized with tetraethylenepentamine called OCNT-TEPA using in vitro assays in murine macrophage cells linage J774 A.1. METHODS: OCNT-TEPA was characterized by transmission electron microscopy (TEM) and high resolution TEM (HR-TEM), scanning electron microscopy (SEM), zeta potential and dynamic light scattering (DLS), and its cytotoxic effects were evaluated at 24 and 48 hours by cell viability assays (MTT and NR), morphology and cell recovery (optic microscopy and clonogenic assay), formation of reactive oxygen (ROS) and nitric oxide (NO) species, inflammatory profile (IL-6 and TNF cytokines), mitochondrial membrane potential analysis (MMP), activation of the caspase 3 pathway and cell death (flow cytometry). RESULTS: The data showed a significant decrease in cell viability, increased production of ROS and NO, alteration of mitochondrial membrane potential, increased levels of inflammatory cytokines, alteration of cell morphology, activation of the Caspase 3 pathway and consequently cell death, in the highest concentrations of OCNT-TEPA tested in the periods of 24 and 48 hours. CONCLUSION: The analyses showed that OCNT-TEPA has a dose-dependent cytotoxic profile, which may be harmful to murine macrophages (J774 A.1) and may represent a health risk.

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Section: Discussionsupporting

confidence: 86%

Cytotoxic Effects Caused by Functionalized Carbon Nanotube in Murine Macrophages.

Godoy

Rodolpho

Fragelli

et al. 2022

Cell Physiol Biochem

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“…Internalization of NPs is a relevant mechanism also for cytotoxicity. Indeed, the capability of some metal or metal oxide NPs, especially Ag NPs, to exert their adverse effects through the intracellular release of metal ions after internalization is largely reported [ 38 , 39 , 40 , 41 ]. Internalized NPs might interact with cellular macromolecules according to their affinity or by generating ROS [ 41 , 42 ].…”

Section: Discussionmentioning

confidence: 99%

Preliminary Toxicological Analysis in a Safe-by-Design and Adverse Outcome Pathway-Driven Approach on Different Silver Nanoparticles: Assessment of Acute Responses in A549 Cells

Motta

Gualtieri

Saibene

et al. 2023

Toxics

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Silver nanoparticles (Ag NPs) are among the most widely used metal-based nanomaterials (NMs) and their applications in different products, also as antibacterial additives, are increasing. In the present manuscript, according to an adverse outcome pathway (AOP) approach, we tested two safe-by-design (SbD) newly developed Ag NPs coated with hydroxyethyl cellulose (HEC), namely AgHEC powder and AgHEC solution. These novel Ag NPs were compared to two reference Ag NPs (naked and coated with polyvinylpyrrolidone—PVP). Cell viability, inflammatory response, reactive oxygen species, oxidative DNA damage, cell cycle, and cell–particle interactions were analyzed in the alveolar in vitro model, A549 cells. The results show a different toxicity pattern of the novel Ag NPs compared to reference NPs and that between the two novel NPs, the AgHEC solution is the one with the lower toxicity and to be further developed within the SbD framework.

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Metal Oxide Nanoparticles and Graphene‐Based Nanomaterials

Cavallo

Chiarella

Fresegna

et al. 2023

Impact of Engineered Nanomaterials in Genomics and Epigenomics

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Toxicological effects of silver nanoparticles and cadmium chloride in macrophage cell line (RAW 264.7): An in vitro approach

Cited by 5 publications

References 67 publications

Cytotoxic Effects Caused by Functionalized Carbon Nanotube in Murine Macrophages.

Cytotoxic Effects Caused by Functionalized Carbon Nanotube in Murine Macrophages.

Preliminary Toxicological Analysis in a Safe-by-Design and Adverse Outcome Pathway-Driven Approach on Different Silver Nanoparticles: Assessment of Acute Responses in A549 Cells

Metal Oxide Nanoparticles and Graphene‐Based Nanomaterials

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