Toxicological effects of multi-walled carbon nanotubes on Saccharomyces cerevisiae: The uptake kinetics and mechanisms and the toxic responses

Zhu, Song; Zhu, Bin; Huang, Aiguo; Hu, Yang; Wang, Gao‐Xue; Ling, Fei

doi:10.1016/j.jhazmat.2016.07.049

Cited by 54 publications

(57 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Non-commercial grade GO and O-SWCNT, also induced ROS with a similar concentration to the one tested here, although the exposure time tested in both cases was 24 h instead of 2 h [52,54]. However, the oxidative stress provoked by MWCNT in yeast seem to be lower than that observed in the present study for GO and GOC or that previously observed for other carbon derived nanoparticles [53]. presence of both carbon nanoparticles.…”

Section: Determination Of Saccharomyces Cerevisiae Cells Response To supporting

confidence: 67%

Section: Determination Of Saccharomyces Cerevisiae Cells Response To mentioning

confidence: 93%

“…The effect on S. cerevisiae viability of non-commercial grade graphene oxide nanoparticles was also tested in a recent study, and the fungus mortality was found to be close to 20% in the presence of 600 mg L −1 [52]. Also, the toxicological potential of other carbon nanomaterials toward S. cerevisiae was reported, such as multi-walled carbon nanotubes (MWCNTs) or oxidized single-walled carbon nanotubes (O-SWCNTs), which induced significant yeast mortality at 400 mg L −1 (6.1%) and 188.2 mg L −1 (approximately 11%) respectively [53,54]. To evaluate whether GO and GOC were able to induce oxidative stress in S. cerevisiae, cells growing at exponential phase were exposed to 160 and 800 mg L −1 of the nanomaterials, for 24 h. As shown in the Figure 8, the oxidative stress levels were significantly increased in S. cerevisiae in the To evaluate whether GO and GOC were able to induce oxidative stress in S. cerevisiae, cells growing at exponential phase were exposed to 160 and 800 mg L −1 of the nanomaterials, for 24 h. As shown in the Figure 8, the oxidative stress levels were significantly increased in S. cerevisiae in the presence of both carbon nanoparticles.…”

Section: Determination Of Saccharomyces Cerevisiae Cells Response To mentioning

confidence: 93%

See 2 more Smart Citations

Interaction Analysis of Commercial Graphene Oxide Nanoparticles with Unicellular Systems and Biomolecules

Domi

Rumbo

García–Tojal

et al. 2019

IJMS

View full text Add to dashboard Cite

The ability of commercial monolayer graphene oxide (GO) and graphene oxide nanocolloids (GOC) to interact with different unicellular systems and biomolecules was studied by analyzing the response of human alveolar carcinoma epithelial cells, the yeast Saccharomyces cerevisiae and the bacteria Vibrio fischeri to the presence of different nanoparticle concentrations, and by studying the binding affinity of different microbial enzymes, like the α-l-rhamnosidase enzyme RhaB1 from the bacteria Lactobacillus plantarum and the AbG β-d-glucosidase from Agrobacterium sp. (strain ATCC 21400). An analysis of cytotoxicity on human epithelial cell line A549, S. cerevisiae (colony forming units, ROS induction, genotoxicity) and V. fischeri (luminescence inhibition) cells determined the potential of both nanoparticle types to damage the selected unicellular systems. Also, the protein binding affinity of the graphene derivatives at different oxidation levels was analyzed. The reported results highlight the variability that can exist in terms of toxicological potential and binding affinity depending on the target organism or protein and the selected nanomaterial.

show abstract

Section: Determination Of Saccharomyces Cerevisiae Cells Response To supporting

confidence: 67%

Section: Determination Of Saccharomyces Cerevisiae Cells Response To mentioning

confidence: 93%

Section: Determination Of Saccharomyces Cerevisiae Cells Response To mentioning

confidence: 93%

See 1 more Smart Citation

Interaction Analysis of Commercial Graphene Oxide Nanoparticles with Unicellular Systems and Biomolecules

Domi

Rumbo

García–Tojal

et al. 2019

IJMS

View full text Add to dashboard Cite

show abstract

“…1, no differences in viability were observed in the selected exposure conditions. Therefore, the selected GN seem to have low toxicity towards S. cerevisiae, being at least lower than that reported for other carbon nanomaterials, such as 2D-graphene oxide (GO), 1D-multi-walled carbon nanotubes (MWCNTs) or 1D-oxidized single-walled carbon nanotubes (O-SWCNTs), which induced significant yeast growth inhibition at lower concentrations (160, 400 and 188.2 mg L −1 respectively) 41,44,46,47 . The impact of 0D-fullerene nanoparticles (nC60) exposure to S. cerevisiae was also studied, with no apparent effect on the growth yield of the fungus, although the nC60 concentration used (31 mg L −1 ) was lower than that used in the previously described studies 48 .…”

Section: Resultsmentioning

confidence: 78%

Toxicological response of the model fungus Saccharomyces cerevisiae to different concentrations of commercial graphene nanoplatelets

Suárez-Diez

Porras

Laguna-Teno

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Graphene nanomaterials have attracted a great interest during the last years for different applications, but their possible impact on different biological systems remains unclear. Here, an assessment to understand the toxicity of commercial polycarboxylate functionalized graphene nanoplatelets (Gn) on the unicellular fungal model Saccharomyces cerevisiae was performed. While cell proliferation was not negatively affected even in the presence of 800 mg L −1 of the nanomaterial for 24 hours, oxidative stress was induced at a lower concentration (160 mg L −1), after short exposure periods (2 and 4 hours). no DnA damage was observed under a comet assay analysis under the studied conditions. in addition, to pinpoint the molecular mechanisms behind the early oxidative damage induced by Gn and to identify possible toxicity pathways, the transcriptome of S. cerevisiae exposed to 160 and 800 mg L −1 of Gn was studied. Both GN concentrations induced expression changes in a common group of genes (337), many of them related to the fungal response to reduce the nanoparticles toxicity and to maintain cell homeostasis. Also, a high number of genes were only differentially expressed in the GN800 condition (3254), indicating that high GN concentrations can induce severe changes in the physiological state of the yeast.

show abstract

“…To confirmt hat the disassembly of PLLf rom the rGO/(PLL/ PASP) 3 @DOX nanocomposites could further destroyt he membrane of HeLa cells, HeLa cells werei ncubated with pure PASP, free DOX and rGO/(PLL/PASP) 3 @DOXn anocomposites for 24 h. Trypan blue was then used to determine cell membrane integrity.T he trypan blue can only enter into cells with ab roken membrane and are revealed as ab lue mark under an optical microscope. [46,47] As shown in Figure S10, trypan blue could go into the HeLa cells incubated with free DOX and rGO/(PLL/ PASP) 3 @DOX, whereas it didn ot enter either the control group or HeLa cells incubated with pure PASP,i ndicating that the membrane of the HeLa cells was destroyedb yt he free DOX or rGO/(PLL/PASP) 3 @DOX. On the other hand, compared with free DOX, more trypanb lue enteredi nto the HeLa cells incubated with rGO/(PLL/PASP) 3 @DOX.…”

Section: Intracellular Uptake and Cell Toxicitymentioning

confidence: 99%

The Fabrication of rGO/(PLL/PASP)₃@DOX Nanorods with pH‐Switch for Photothermal Therapy and Chemotherapy

Zhang

Meng

et al. 2018

Chemistry A European J

View full text Add to dashboard Cite

The development of well-controlled drug carriers that are stable and highly effective for the delivery of anticancer agents is challenging. Herein, we report a novel pH-controlled drug delivery system, utilizing reducing graphene oxide (rGO)-polymer self-assembly films as carriers, for the preparation of effective drug nanorods and nanoparticles. In this system, the rGO-polymer carriers were constructed by the alternating assembly of poly-l-lysine (PLL) and polyaspartic acid (PASP) around the rGO sheets. Furthermore, the rGO-polymer cores, which possess a positively charged surface as the desired template, could assemble with negatively charged doxorubicin (DOX) via electrostatic interactions. The DOX embedding efficiency and the morphology of the drug nanocomposites could be controlled by the number of rGO-polymer bilayers and concentration of the rGO-polymer bilayers and the initial DOX concentration. Importantly, the release of DOX could be regulated by controlling the pH and by using a NIR laser. Under acidic conditions, the interactions between the PASP layer and DOX molecules can be broken, resulting in gradual release of the DOX molecules. Upon NIR irradiation, the release of DOX could be further accelerated and a photothermal effect from rGO induced. Cellular uptake and cytotoxicity experiments indicate that the drug nanocomposites possess effective anticancer activity. Thus, in this work, we present a useful strategy for the fabrication of pH-responsive drug nanocomposites for combined photothermal and chemical therapy. The nanocomposite can be used as a potential drug delivery system for practical cancer treatment.

show abstract

Toxicological effects of multi-walled carbon nanotubes on Saccharomyces cerevisiae: The uptake kinetics and mechanisms and the toxic responses

Cited by 54 publications

References 51 publications

Interaction Analysis of Commercial Graphene Oxide Nanoparticles with Unicellular Systems and Biomolecules

Interaction Analysis of Commercial Graphene Oxide Nanoparticles with Unicellular Systems and Biomolecules

Toxicological response of the model fungus Saccharomyces cerevisiae to different concentrations of commercial graphene nanoplatelets

The Fabrication of rGO/(PLL/PASP)₃@DOX Nanorods with pH‐Switch for Photothermal Therapy and Chemotherapy

Contact Info

Product

Resources

About

Toxicological effects of multi-walled carbon nanotubes on Saccharomyces cerevisiae: The uptake kinetics and mechanisms and the toxic responses

Cited by 54 publications

References 51 publications

Interaction Analysis of Commercial Graphene Oxide Nanoparticles with Unicellular Systems and Biomolecules

Interaction Analysis of Commercial Graphene Oxide Nanoparticles with Unicellular Systems and Biomolecules

Toxicological response of the model fungus Saccharomyces cerevisiae to different concentrations of commercial graphene nanoplatelets

The Fabrication of rGO/(PLL/PASP)3@DOX Nanorods with pH‐Switch for Photothermal Therapy and Chemotherapy

Contact Info

Product

Resources

About

The Fabrication of rGO/(PLL/PASP)₃@DOX Nanorods with pH‐Switch for Photothermal Therapy and Chemotherapy