Toxicological comparisons of Tetrahymena species, end points and growth media: Supplementary investigations to the pilot ring test

Pauli, W.; Berger, Sandra L.

doi:10.1016/s0045-6535(97)00171-9

Cited by 16 publications

(8 citation statements)

References 8 publications

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“…They concluded that the Tetrahymena test is recommended for ecotoxicological screening purposes, being an ecologically relevant supplement to aquatic toxicity testing. Population growth impairment is the most sensitive and often-used sublethal toxic endpoint [21] and reveals predominantly higher sen- sitivities than tests reflecting viability, respiration or the chemosensory responses [22]. Moreover, growth/mortality bioassay using microscopic observation of the morphological changes, at low magnification, is a simple and reproducible technique that does not require technical expertise or operational expenses [15].…”

Section: Discussionmentioning

confidence: 99%

A comparative study using a fluorescence-based and a direct-count assay to determine cytotoxicity in Tetrahymena pyriformis

Dias

Lima

2002

Research in Microbiology

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A novel cellular cytotoxicity assay using two fluorescent dyes was developed as an alternative method to the standard direct count of viable protozoa under light microscopy. The compound calcein AM is a non-fluorescent substance that diffuses passively across intact cell membranes and is converted by intracellular esterases to the green fluorescent calcein, which is retained in viable cells. The addition of EthD-1 that binds to DNA stained nuclei of dead cells red. The experiments were carried out in order to assess viability in the freshwater ciliate Tetrahymena pyriformis after exposure to eight surfactants, two of each representing one of four ionic class (non-ionic, anionic, cationic and amphoteric), and two heavy metals, copper and zinc, at several concentrations. In earlier time exposure, less than one hour of contact with surfactants at sublethal concentrations, the fluorescent method is more sensitive and provides more accurate results than direct counting under light microscopy. In contrast, with increasing time exposure, the results obtained by the two methods were similar. Calcein was shown to be a poor viability marker in the presence of zinc and copper since the fluorescence intensity was affected by the metal presence. However, the fluorescent method offers new opportunities to use advanced techniques, such as flow cytometry, to assess cytotoxicity in protozoa.

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Section: Discussionmentioning

confidence: 99%

A comparative study using a fluorescence-based and a direct-count assay to determine cytotoxicity in Tetrahymena pyriformis

Dias

Lima

2002

Research in Microbiology

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“…A tubes technique was also used to study the growth dynamics of the T. pyriformis population and determine the toxicity of a substance by calculating the 9 h median inhibitory concentration (9 h-IC 50 ). It was adapted from the methods developed by Larsen et al (1997) and Pauli and Berger (1997) to reduce both the volume of protozoan culture and the quantities of molecules used. This technique consists in taking 15 mL sterile polystyrene round-bottom tubes to inoculate T. pyriformis and then monitoring culture growth by photometric readings.…”

Section: Population Growth Rate Test (Experiments With T Pyriformis)mentioning

confidence: 99%

Assessment of the potential toxicity of herbicides and their degradation products to nontarget cells using two microorganisms, the bacteria Vibrio fischeri and the ciliate Tetrahymena pyriformis

Bonnet¹,

Bonnemoy

Dusser³

et al. 2007

Environmental Toxicology

107

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ABSTRACT:The potential toxicity of several herbicides-alachlor, diuron and its photo and biotransformation products, glyphosate and its metabolite aminomethyl phosphonic acid (AMPA)-to nontarget cells was assessed using two microorganisms frequently used in ecotoxicology, Vibrio fischeri and Tetrahymena pyriformis. Toxicity assays involved the Microtox 1 test, the T. pyriformis population growth impairment test employing three different processes (flasks, tubes, microplates), and the T. pyriformis nonspecific esterase activities test. Several IC 50 or EC 50 values are reported for each molecule. Alachlor exerted a toxic effect on the two nontarget cells used. The results for diuron and its photo and biotransformation products indicated that most of the metabolites presented nontarget toxicity higher than that of diuron. Glyphosate and AMPA had a less negative effect on T. pyriformis than on V. fischeri. Nevertheless, in all cases, glyphosate was found to be more toxic than AMPA. Comparison analysis of the sensitivity of the different tests showed that, in general, tests using the eukaryotic cell (T. pyriformis) were more sensitive than test using the prokaryotic cell (V. fischeri), and that a population growth criterion is more sensitive than an enzymatic criterion. The three different processes that could be used to evaluate effects on population growth rate were equally sensitive for the herbicides tested. A significant correlation between toxicity data and the hydrophobicity of the chemicals could only be established with the growth population test. This study demonstrates that it is essential to assess the toxicity of the metabolites formed to complete a more comprehensive study of the environmental impact of a polluting agent. # 2007 Wiley Periodicals, Inc. Environ Toxicol 22: 78-91, 2007.

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“…Accepted test procedures are not yet available (van Gestel and van Straalen 1994;Kammenga et al, 1996;Pratt et al, 1997). Among the protozoans, however, the ciliates ¹etrahymena pyriformis, ¹etrahymena thermophila (Pauli and Berger, 1997;Sauvant et al 1997), Colpoda cucullus, Colpoda in-ata (Pratt et al, 1997), Colpoda steinii (Campbell et al, 1997), and Paramecium aurelia have been developed as test animals, as have the nematode species Caenorhabditis elegans (Traunspurger et al, 1997), Panagrellus silusiae and Plectus acuminatus. Dutka et al (1996) used a Panagellus redivivus nematode test to assess the toxicity of water and sediment samples from the Temuco and Rapel rivers in Chile.…”

Section: Test Methods With Soil Faunamentioning

confidence: 99%

Ecotoxicity Tests for Compost Applications

Kapanen

Itävaara

2001

Ecotoxicology and Environmental Safety

161

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Toxicological comparisons of Tetrahymena species, end points and growth media: Supplementary investigations to the pilot ring test

Cited by 16 publications

References 8 publications

A comparative study using a fluorescence-based and a direct-count assay to determine cytotoxicity in Tetrahymena pyriformis

A comparative study using a fluorescence-based and a direct-count assay to determine cytotoxicity in Tetrahymena pyriformis

Assessment of the potential toxicity of herbicides and their degradation products to nontarget cells using two microorganisms, the bacteria Vibrio fischeri and the ciliate Tetrahymena pyriformis

Ecotoxicity Tests for Compost Applications

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