Toxicological, chemopreventive, and cytotoxic potentialities of rare vegetal species and supporting findings for the Brazilian Unified Health System (SUS)

Chemotherapeutic and Safety Profile of a Fraction from Mimosa caesalpiniifolia Stem Bark

“…DFMC did not produce hemolysis and showed higher potential as a cytotoxic agent than betulinic acid for the SF-295 and HL-60 lines [20,36], corroborating the findings described here for S180 cells. Phytochemical investigation of extracts from Mimosa species revealed the existence of terpenes, flavonoids, steroids, phenols (especially tannins), and fatty acid derivatives in different parts of the plant (leaves, fruits, flowers, branches, and stem bark) [36][37][38][39][40], mainly betulinic acid, lupeol, phytol, lactic acid, α-tocopherol, stigmasterol, β-sitosterol, sitostenone, and stigmasta-4,22-dien-3-one, which had been identified in dichloromethane, ethanolic, and hexane fractions of leaves and barks from M. caesalpiniifolia [15,36,40], suggesting that the antiproliferative potential of DFMC may be attributed, at least in part, to its chemopreventive action. In this context, Silva et al [15] stated the scavenger activity of M. caesalpiniifolia extracts, whose presence of phenolic compounds was confirmed by ultraviolet-visible spectroscopy and thin-layer chromatography.…”

“…A voucher sample (26.824) was deposited at Graziela Barroso Herbarium at Federal University of Piauí (Teresina, Piauí, Brazil). Air-dried plant material was pulverized, extracted with ethanol, concentrated under reduced pressure, and subjected to successive partitioning with dichloromethane as described by Silva et al [15]. Previously, we isolated betulinic acid [3β-hydroxy-lup-20(29)-en-28-oic acid] and verified it as the main compound in the dichloromethane fraction (∼70.3%), as demonstrated by TLC (thin-layer chromatography), GC-qMS (gas chromatograph quadrupole mass spectrometer), HRAPCIMS (high-resolution atmospheric pressure chemical ionization mass spectrometer), 1 H-and 13 C-nuclear magnetic resonance, and DEPT analysis [15,23].…”

“…Air-dried plant material was pulverized, extracted with ethanol, concentrated under reduced pressure, and subjected to successive partitioning with dichloromethane as described by Silva et al [15]. Previously, we isolated betulinic acid [3β-hydroxy-lup-20(29)-en-28-oic acid] and verified it as the main compound in the dichloromethane fraction (∼70.3%), as demonstrated by TLC (thin-layer chromatography), GC-qMS (gas chromatograph quadrupole mass spectrometer), HRAPCIMS (high-resolution atmospheric pressure chemical ionization mass spectrometer), 1 H-and 13 C-nuclear magnetic resonance, and DEPT analysis [15,23]. Plant samplings were authorized by the System of Authorization and Information on Biodiversity (SISBIO/BAMA #50090-3) and registered in SisGen (Sistema Nacional de Gestão do Patrimônio Genético e do Conhecimento Tradicional Associado #ABC4AC2) according to Brazilian legislation (Federal Law No 13,123/ 2015).…”

“…ese investigations were performed using the fraction composed of a mixture of molecules because such preparations represent the main folk approach of consumption by the Brazilian population [15].…”

“…A Brazilian dry region named "Caatinga" dominates 7% of the Brazilian territory and is an exclusive biome. It generates particular environmental conditions for steppe climate-adapted flora and fauna and a high number of rare and endemic taxa [9,10], exhibiting many vegetal families, such as Fabaceae, Anarcadiaceae, Caryocaraceae, Rhamnaceae, Chrysobalanaceae, Clusiaceae, Connaraceae, Sapindaceae, Annonaceae, Combretaceae, and Bignoniaceae [11][12][13][14][15]. Among them, inflorescences from Mimosa caesalpiniifolia Benth.…”