Toxicological assessment of PEGylated single-walled carbon nanotubes in early developing zebrafish

Cordeiro, Marcos Freitas; Girardi, Felipe A.; Gonçalves, Carla O.F.; Peixoto, Carolina; Bosco, Lidiane Dal; Sahoo, Sangram K.; Santos, Adelina P.; Fantini, C.; Bruch, Gisele Eva; Horn, Ana Paula; Barros, Daniela M.

doi:10.1016/j.taap.2018.03.031

Cited by 16 publications

(5 citation statements)

References 29 publications

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“…Not only length is an important variable, but thicker MWCNT are also associated with higher toxicity (Adenuga et al 2013). In previous works by our research group we observed that even very thin CNT can be unable to cross the chorionic membrane, still leading to significant toxic effects not associated with the direct contact of the nanomaterials with the developing organism, including asphyxiation as a result of clogged chorionic pores (Cheng et al 2007, Girardi et al 2016, Cordeiro et al 2018. Even though the MWCNT-Pyr-PEG used was estimated to measure around 10 nm in diameter and the chorion pores are estimated to measure around 230 nm in diameter (Pelka et al 2017), it is still likely that a significant fraction of the MWCNT-Pyr-PEG sample would be unable to successfully cross the chorion because of its adhesive surface.…”

Section: Discussionmentioning

confidence: 94%

Multi-walled carbon nanotubes functionalized with pyrene-PEG via π–π interactions: toxicological assessment in zebrafish embryos

et al. 2020

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Multi-walled carbon nanotubes (MWCNT) have many promising biological applications, even though functionalization is needed for better biocompatibility. Functionalization of MWCNT with polyethylene glycol (PEG) is a promising and widely studied approach, but the best PEGylation method is still under investigation. In this work, we have tested the biological implications of MWCNT functionalized via π-stacking with pyrene-PEG (MWCNT-Pyr-PEG) in zebrafish embryos. As Pyr toxicity is well documented and represents a major concern for the safety of the proposed approach, we have also tested the effects of the exposure to the isolated conjugate (Pyr-PEG). The resulting suspensions were stable in saline medium and well dispersed. Zebrafish embryos at 24 h post-fertilization (hpf) were dechorionated and randomly assigned to seven experimental groups (n = 50 per group): control, MWCNT-Pyr-PEG at 0.2, 2.0, and 20.0 mg l −1 , and Pyr-PEG at the same concentrations, and exposures were performed in 96-well plates. Specimens were observed for heart rate, malformations, body length, mortality, traveled distance, and number of new movements. Heart rate was reduced in embryos exposed to any tested concentration of MWCNT-Pyr-PEG, while this effect was observed with Pyr-PEG from 2 mg l −1 . The highest concentration of MWCNT-Pyr-PEG also led to increased occurrence of malformations, shortened body length and reduced traveled distance. The functionalization approach shows promise due to the stability in saline media, even though toxic effects were observed in the highest tested concentrations, being the MWCNT the main actors underlying these outcomes.

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Section: Discussionmentioning

confidence: 94%

Multi-walled carbon nanotubes functionalized with pyrene-PEG via π–π interactions: toxicological assessment in zebrafish embryos

et al. 2020

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“…102,103 In this context, D. rerio is a suitable model for nanotoxicity. 13,104 Half of the studies using nanomaterials in Brazil investigated the toxicity of carbon nanotubes with different functionalizations 75,[105][106][107][108][109] and the toxicity of TiO 2 nanoparticles and multiwalled carbon nanotube hybrids. 110 Others studied the toxicity of silver nanoparticles in zebrafish and their bacterial communities, 111 iron oxide nanoparticles, 74,76 graphene oxide associated with organic matter 112 and the interaction of fullerene and arsenium in zebrafish liver cells.…”

Section: Research Areasmentioning

confidence: 99%

Zebrafish as an Emerging Model System in the Global South: Two Decades of Research in Brazil

et al. 2020

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The zebrafish (Danio rerio) is an emerging model system in several research areas worldwide, especially in the Global South. In this context, the present study revised the historical use and trends of zebrafish as experimental models in Brazil. The data concerning the bibliometric parameters, research areas, geographic distribution, experimental design, zebrafish strain, and reporter lines, as well as recent advances were revised. In addition, the comparative trends of Brazilian and global research were discussed. Revised data showed the rapid growth of Brazilian scientific production using zebrafish as a model, especially in three main research areas (Neuroscience &and Behavior, Pharmacology and Toxicology, and Environment/Ecology). Studies were conducted in 19 Brazilian states (70.37%), confirming the wide geographic distribution and importance of zebrafish research. Results indicated that research related to toxicological approaches are widespread in Global South countries such as Brazil. Studies were performed mainly using in vivo tests (89.58%) with adult fish (59.75%) and embryos (30.67%). Moreover, significant research gaps and recommendations for future research are presented. The present study shows that the zebrafish is a suitable vertebrate model system in the Global South.

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“…SWCNTs functionalized with polyethylene glycol are toxic without being absorbed by the organism and the effects are shown in the mortality, the delay of the hatching, malformations, reductions in body length, the increase in ROS generation and DNA damage (Cordeiro et al, 2018). Otherwise 3-4 hpf zebrafish embryos show protective effects of MWCNTs by reducing the expected estrogenic response of 17βestradiol exposures (Yan et al, 2018b).…”

Section: Carbon Nanotubesmentioning

confidence: 99%

Toxicology of Nanomaterials on Zebrafish

León¹,

Cotto²,

Márquez³

2019

American Journal of Engineering and Applied Sciences

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Increases in the use and the progressive manufacture of new Engineered Nanomaterials (ENMs) lead to inquire about their impact on the environment. Due to the small size, high reactivity inside organisms and the unusual physicochemical properties of the ENMs, the predictions of toxicity are very complex. The zebrafish (Danio rerio) has been granted as a practical alternative to study the toxicity of ENMs. In this article the toxic effects of silver nanoparticles, titanium oxide nanoparticles, zinc oxide nanoparticles, carbon nanotubes, copper nanoparticles, gold nanoparticles, cadmium nanoparticles and nano plastics were reviewed trough the most recent literature available. Every ENMs should be studied in depth independently, considering co-exposures, environmental matrices, the effects of variations in size and concentrations, the potential effects of prolonged exposure, the coverage of ENMs, specific organism and targeted organs. This information will help to identify deficiencies in research trends and reinforce the safest ways to use ENMs.

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Toxicological assessment of PEGylated single-walled carbon nanotubes in early developing zebrafish

Cited by 16 publications

References 29 publications

Multi-walled carbon nanotubes functionalized with pyrene-PEG via π–π interactions: toxicological assessment in zebrafish embryos

Multi-walled carbon nanotubes functionalized with pyrene-PEG via π–π interactions: toxicological assessment in zebrafish embryos

Zebrafish as an Emerging Model System in the Global South: Two Decades of Research in Brazil

Toxicology of Nanomaterials on Zebrafish

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