Toxicological analysis of the chronic consumption of diheptanoin and triheptanoin in rats

Abstract

The aim of this study was to synthesise glycerol esters from heptanoic acid and to evaluate the toxicity of their chronic consumption in rats. The synthesised product (64% triheptanoin, 34% diheptanoin and 2% monoheptanoin) was called experimental oil. Newly weaned male Wistar rats (n = 3 · 10) were fed with control diet AIN-93, control with 30% substitution of soybean oil with experimental oil (TAGC 7 30%) or control with substitution of 50% (TAGC 7 50%), for 9 months. The experimental oil did not affect the growth, lipid digestibility or hepatic and renal function, and the lipid profile serum analysis. Additionally, it exhibited a hepato-protector effect in group TAGC 7 50%. In conclusion, the chronic consumption of di-and triheptanoin was not toxic in rats, suggesting that they may be used in the treatment of disorders in which more easily digestible lipids are required.

High‐fat diet based on trienantin has no adverse metabolic effects in rats

“…As for the markers for hepatic function and injury, these findings confirm the data previously described by our group in rats, in which a partial substitution with trienantin for LCT in a standard diet [14] did not demonstrate alterations in the above parameters. Kono et al [39] observed a hepatoprotector effect of MCT in a group of animals fed with 5 g/kg of medium-chain fatty acids, which showed a reduced mortality rate on being infected with a Gram-negative bacterium.…”

“…It was demonstrated in a single study of toxicological evaluation in rats that trienantin in a normal-fat diet produced satisfactory coefficients of digestibility and feed efficiency, and no evidence was found of hepatotoxicity or alterations in intestinal or renal morphology, after 9 months [14]. Therefore, as in a few other studies, this indicated favorable evidence for the therapeutic use of trienantin.…”

“…In truth, the composition of the diet can promote the occurrence of hepatic steatosis, a principle used in the determination of experimental models of steatosis and steato-hepatitis [42,43]. In this sense, cases of hepatic steatosis associated with the use of the reference diet AIN-93 have been reported [14,44]. For this reason, the influence of the formulation of the control diet cannot be disregarded in the present study.…”

“…Hepatic steatosis, when present, was classified in accordance with the distribution pattern of lipid infiltrations in the hepatic parenchyma as: light, lightly moderate, moderate and severe, following the classification adopted in a previous work [14] (Fig. 1).…”

Perfil em ácidos graxos hepáticos de ratos com esteatose induzida pela dieta AIN-93 atenuada pela substituição parcial do óleo de soja por dieptanoína e trieptanoína

“…(1) e, posteriormente, no estudo de Ataíde e cols. (2), que mostraram que esses acilgliceróis exercem um potencial efeito hepatoprotetor contra a esteatose, numa feição dosedependente. O perfil em ácidos graxos (AG) de lipídios dietéticos determina a incorporação e a composição em AG das membranas biológicas, alterando diversas funções celulares (3), principalmente pela modulação dos AG esterificados aos fosfolipídios de membrana (4).…”