Toxicogenetic of tebuconazole based fungicide through Lactuca sativa bioassays

Aragão, Francielen Barroso; Duarte, Ian Drumond; Fantinato, Dayana Effgen; Galter, Iasmini Nicoli; Silveira, Graciele Lurdes; Reis, Gabriela Barreto dos; Andrade-Vieira, Larissa Fonseca; Matsumoto, Sílvia Tamie

doi:10.1016/j.ecoenv.2021.111985

Cited by 11 publications

(4 citation statements)

References 36 publications

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“…The tendency of those species as the main choice for toxicity testing is related to several factors, including the relatively short duration of observation, relatively low cost, good knowledge of their biology and ecology, relatively easy manipulation and maintenance in the laboratory, small body size allowing accommodation in small beakers or microplates, and high adaptability to numerous test conditions [ 33 , 34 , 35 , 36 ]. Lettuce is a very popularly used plant for growth inhibition assays [ 28 , 44 , 45 , 46 , 47 , 48 ]. Its relatively easy and fast growth makes it one of the main choices for toxicology testing.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Toxicity of Crude Phlorotannins and Phloroglucinol Using Different Model Organisms

Harwanto

Negara

Tirtawijaya

et al. 2022

Toxins

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Phlorotannins have been proven to contain numerous bioactive compounds that have potential to be applied in variety industries, including cosmetics, functional foods, nutraceuticals, environmental management, and medicine. The larvicidal and growth-inhibiting properties of phlorotannins have been extensively studied in various organisms. However, the toxicity of the phloroglucinol oligomer of phlorotannin is unclear, especially in Artemia salina, Daphnia magna, Lactuca sativa, and Chlorella vulgaris, which are commonly used in many bioassays. Therefore, research using these four organisms should be designed to provide basic information about the toxic effects of phlorotannins and phloroglucinol. This study aimed to evaluate the larvicidal and inhibitory properties of phlorotannins and phloroglucinol on A. salina, D. magna, L. sativa, and C. vulgaris. Phlorotannin extract and phloroglucinol were administered at various concentrations to each test organism. The survival rate of A. salina nauplii and D. magna neonates was observed every 24 h to 72 h, whereas the L. sativa seed germination and inhibition rate of C. vulgaris were observed up to 96 h. The results showed that the 24 h LC50 of phlorotannin on A. salina and D. magna were 10.67 and 1.32 mg/mL, respectively. The germination inhibition of L. sativa was 53.3% with a seed growth of less than 4 mm after 96 h upon exposure to 1 mg/mL of phlorotannin. Freshwater and seawater C. vulgaris experienced yield inhibition of 39.47 and 43.46%, respectively, when 2 mg/mL of phlorotanin was added. These results indicate that phlorotannin affects the survival and growth of the test organisms, so its use as a pesticide, herbicide, and algaecide agent for environmental and aquaculture applications can be further studied.

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Section: Introductionmentioning

confidence: 99%

Evaluation of Toxicity of Crude Phlorotannins and Phloroglucinol Using Different Model Organisms

Harwanto

Negara

Tirtawijaya

et al. 2022

Toxins

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“…Higher plants bioassays are efficient to identify the toxicity of environmental pollutants [11][12][13][14]. Macroscopic phytotoxicity tests are carried out through bioassays involving the germination of seeds of model species in addition to root and shoot growth of these model plants.…”

Section: Introductionmentioning

confidence: 99%

“…Cytogenetic toxicity bioassays are tests that can be associated with macroscopic tests [15]. They are based on the assessment of the cell cycle, allowing the observation of events throughout mitosis [11,16], and can identify changes in the cell cycle by detecting abnormalities in the rate of cell division and also the presence of chromosomal and nuclear alterations [13].…”

Section: Introductionmentioning

confidence: 99%

Efects of a S-Metolachlor Based Herbicide on Two Plant Models: Zea Mays L. And <i>Lactuca Sativa L.</i>

Silva¹,

Palmieri²,

Andrade-Vieira³

2022

Preprint

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Brazil is the number-one country in pesticide consumption, and corn is the second most cultivated crop in the country. Chemical control of weeds associated with corn cultivation is performed by application of herbicides with pre- and postemergence action, such as S-metolachlor. Currently, the toxicity of herbicides is a task of great concern. In this regard, the present study aimed to evaluate the effects of an S-metolachlor-based herbicide through bioassays with the plant model Lactuca sativa L. (lettuce) and Zea mays L. (maize). The herbicidal test solutions containing 7.5, 15, 30, 60, 120, 240, 360, 480, 600, and 720 mg L-1 of the active ingredient S-metolachlor were prepared from commercial products. Distilled water was used as a negative control, and aluminum was used as a positive control. Macroscopic analyses (germination and growth) were performed for the two species, and microscopic analyses (chromosomal and nuclear changes) were performed for L. sativa. Negative interference of the S-metolachlor-based herbicide on lettuce was observed for all macroscopic and microscopic parameters tested. In maize, there was no significant interference in germination; however, the herbicide interferes negatively in seedling development. In brief, the herbicide based on S-metolachlor has phytotoxic potential, just as discussed.

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“…). In addition, mutations in cyclin and kinase proteins involved in the control of cell division also cause inhibition of mitotic index and mitotic stages (Marc et al 2002(Marc et al , 2004Aragão et al 2021;Altman 2018). We believe that Fluquinconazole used in this study effectively decreased the mitotic index in A. cepa root tip cells compared to the control.…”

mentioning

confidence: 99%

Evaluation of the cytotoxic and genotoxic effects of 2 Fluquinconazole using Allium cepa L. as a bioindicator

Türkoğlu

2023

Preprint

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Fluquinconazole is the active substance of a synthetic fungicide which is used extensively in agricultural areas in the world and Turkey. In this study, 30, 60, 90 and 100 mg/l doses of the substance were applied to Allium cepa root tips for 12, 24 and 48 hours. Distilled water was used as a negative control while methyl methane sulfonate (MMS, 10 ppm) was used as a positive control. As a result of the application, it was observed that the substance caused mitotic inhibition by decreasing the mitotic index, leading to changes in mitotic stage ratios. It was also observed that this substance caused chromosomal (anaphase bridges, stickiness, c-mitosis, laggards, and breakages) and nuclear abnormalities (binucleus and micronucleus). At the end of the statistical analysis and examinations, it was detected that the substance caused cytotoxic and genotoxic effects.

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Toxicogenetic of tebuconazole based fungicide through Lactuca sativa bioassays

Cited by 11 publications

References 36 publications

Evaluation of Toxicity of Crude Phlorotannins and Phloroglucinol Using Different Model Organisms

Evaluation of Toxicity of Crude Phlorotannins and Phloroglucinol Using Different Model Organisms

Efects of a S-Metolachlor Based Herbicide on Two Plant Models: Zea Mays L. And <i>Lactuca Sativa L.</i>

Evaluation of the cytotoxic and genotoxic effects of 2 Fluquinconazole using Allium cepa L. as a bioindicator

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