Toxicogenetic effects of low concentrations of the pesticides imidacloprid and sulfentrazone individually and in combination in in vitro tests with HepG2 cells and Salmonella typhimurium

Bianchi, Jaqueline; Cabral-de-Mello, Diogo Cavalcanti; Marin-Morales, Maria Aparecida

doi:10.1016/j.ecoenv.2015.05.040

Cited by 48 publications

(27 citation statements)

References 74 publications

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“…Our results showed a predominance of small micronucleus at 100 mg/L and 1000 mg/L, indicating a clastogenic origin of micronucleus caused by this insecticide. This corresponds with the capacity of imidacloprid to induce DNA damage in bone marrow cells of rats, measured by micronucleus and chromosome aberrations (chromosome breaks and stickiness) [57] and in human hepatoma cells (HepG2) in which 76.6% of micronuclei did not present a centromeric signal [58].…”

Section: Genotoxicity Testsmentioning

confidence: 99%

Uptake, distribution in different tissues, and genotoxicity of imidacloprid in the freshwater fish Australoheros facetus

Iturburu

Zömisch

Panzeri

et al. 2016

Enviro Toxic and Chemistry

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The neonicotinoid imidacloprid is under re-evaluation by regulatory agencies because of the poor current information available regarding its potential effects. One of the goals of the present study was to determine imidacloprid uptake and distribution in the freshwater fish Australoheros facetus experimentally exposed for 24 h and 48 h to 100 μg/L, 300 μg/L, and 2500 μg/L. The toxicity of imidacloprid to fish reported in the literature is in the milligrams per liter or gram per liter range, but sublethal effects at micrograms per liter in some groups other than fish have been described. Another goal of the present study was to evaluate imidacloprid's potential genotoxicity and to compare it between the individual compound and a commercial formulation. Concentrations of imidacloprid were measured in water, brain, muscle, gills, gut, liver, and blood by liquid chromatography-tandem mass spectrometry. Imidacloprid was detected in all the tissues tested. Concentrations were higher after 48 h than after 24 h in liver, gills, gut, and muscle, whereas in brain and blood they were similar at both exposure times. Although there was no accumulation, only uptake, of imidacloprid, genotoxicity was observed. In fish exposed to IMIDA NOVA 35 , increased micronucleus frequency at 100 µg/L and 1000 µg/L was detected, whereas in the imidacloprid active ingredient bioassay it increased only at 1000 µg/L imidacloprid. The present findings warn of the possible consequences that fish living in freshwater ecosystems can suffer. Environ Toxicol Chem 2017;36:699-708. © 2016 SETAC.

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Section: Genotoxicity Testsmentioning

confidence: 99%

Uptake, distribution in different tissues, and genotoxicity of imidacloprid in the freshwater fish Australoheros facetus

Iturburu

Zömisch

Panzeri

et al. 2016

Enviro Toxic and Chemistry

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“…Therefore, alternative models to investigate the toxic potential of physic nut varieties with different contents of phorbol ester should be considered. Among the tests available to study the toxic potential of a given compound or residue, plant models stand out for their good sensitivity and low cost (Leme & Marin-Morales, 2009), besides presenting good correlation with data from tests using animal models (Bianchi, Cabral-de-Mello, & Marin-Morales, 2015) or even human cells (Palmieri, et al, 2016).…”

mentioning

confidence: 99%

Cytogenetic bioassays as tools to distinguish between toxic and non-toxic varieties of Jatropha curcas (Euphorbiaceae)

Andrade-Vieira

Botelho

Pinheiro

et al. 2018

RBT

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The tropical and subtropical naturalized physic nut (Jatropha curcas L.), has been explored for biodiesel production in recent times. The oil is extracted from the seeds and, for the production to be feasible, utilization of the residual seed cake is crucial. Although the cake could be employed as a protein source in animal feed, it is rich in phorbol ester, which is toxic for animals. Therefore, breeding programs have been working to reduce or eliminate the phorbol ester content in physic nut. In this context, the present work aimed to evaluate the physic nut oil of toxic and non-toxic varieties (containing known or undetectable amounts of phorbol ester, respectively) with regards to phytotoxicity in a model experiment with Lactuca sativa L. For this, the percentage of germinated seeds was evaluated after 8, 16, 24, 36 and 48 hours of exposure to the treatments with toxic and non-toxic oil at concentrations of 22.5 %, 45 % and 67.5 % of emulsion (physic nut oil energetically mixed with distilled water). Root growth was determined after 48 hours of exposure and the germination speed index was obtained. The different stages of mitotic division as well as possible chromosomal and nuclear alterations were also recorded. The mitotic index was calculated as the number of dividing cells, as a fraction of the total number of cells, and the frequency of chromosome and nuclear alterations, expressed as the percentage of number of alterations divided by the total number of cells. Both varieties exhibited phytotoxicity, inducing significant reductions in percentage of germinated seeds (reduction of 98 %), germination speed index (reduction of 24.44) and root growth (reduction of 8.54 mm). In microscopic analysis, a mitodepressive effect was observed for both oils at the three concentrations used when compared to the negative control; however, it was possible to distinguish between the toxic and the non-toxic varieties based on the more expressive reduction of division promoted by the first, 2.19 %. Significant increments in the frequency of mitotic cells showing chromosome alterations as well, as the presence of condensed nuclei, were observed in the treated cells. However, these parameters were not significantly different from the control in the cells treated with both physic nut oils. In conclusion, the evaluation of root growth and cell division in the plant model L. sativa, can be proposed as an alternative to animal tests to distinguish the varieties with high and low phorbol ester concentration, thus contributing to the detection of toxicity in varieties used in breeding programs. Rev. Biol. Trop. 66(2): 495-502. Epub 2018 June 01.

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“…According to the FISH results, the damage caused by imidacloprid and sulfentrazone resulted from a clastogenic action. No significant result was found with Ames test [36] ( Figure 3). …”

Section: Recents Studies Using Comest Assaymentioning

confidence: 76%

Comet Assay on Toxicogenetics; Several Studies in Recent Years on Several Genotoxicological Agents

Gharsalli¹

2016

J Environ Anal Toxicol

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Toxicogenetic effects of low concentrations of the pesticides imidacloprid and sulfentrazone individually and in combination in in vitro tests with HepG2 cells and Salmonella typhimurium

Cited by 48 publications

References 74 publications

Uptake, distribution in different tissues, and genotoxicity of imidacloprid in the freshwater fish Australoheros facetus

Uptake, distribution in different tissues, and genotoxicity of imidacloprid in the freshwater fish Australoheros facetus

Cytogenetic bioassays as tools to distinguish between toxic and non-toxic varieties of Jatropha curcas (Euphorbiaceae)

Comet Assay on Toxicogenetics; Several Studies in Recent Years on Several Genotoxicological Agents

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