Toxicity testing of heavy metals with theRhizobium-legume symbiosis: High sensitivity to cadmium and arsenic compounds

Neumann, H. O.; Bode-Kirchhoff, Anne; Madeheim, Andrea; Wetzel, Astrid

doi:10.1007/bf02986371

Cited by 29 publications

(8 citation statements)

References 12 publications

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“…With this background in mind, the results of this study showed that both As(III) and As(V) were toxic to L. gibba. As(III) species were more toxic than As(V) species (Neumann et al, 1998). However, Knauer et al (1999 reported that As(V) exhibits higher toxicity to algae than does As(III).…”

Section: Relation Of Chemical Speciation To Toxicitymentioning

confidence: 90%

Toxicity of arsenic species to Lemna gibba L. and the influence of phosphate on arsenic bioavailability

Mkandawire

Lyubun

Kosterin

et al. 2004

Environmental Toxicology

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The toxicity of arsenic (As) species to Lemna gibba L. and the influence of PO(4) (3-) on As bioavailability and uptake were tested in batch culture. L. gibba were exposed to six test concentrations of NaHAsO(4). 7H(2)O and NaAsO(3), with 0, 0.0136, 13.6, and 40 mg L(-1) KH(2)PO(4). In batch culture As toxicity to L. gibba did not relate linearly to As concentration. The growth rate, related to frond number as recommended by OECD and ISO/DIN, was significantly inhibited in fronds exposed to 20-50 microg L(-1) As(III) compared with fronds exposed to As(V). The growth rate was stimulated when plants were exposed to 50-250 microg L(-1) of both As(III) and As(V). After exposure to 300-800 microg L(-1) growth inhibition was significantly higher for As(III) than for As(V), whereas above 800 microg L(-1) As(V) was inhibited the most. The bioaccumulation of As(III) and As(V) was significantly higher for P-deficient cultures (0.98 +/- 0.08 and 1.02 +/- 0.19 g kg(-1), respectively for 0.0136 mg L(-1) PO(4) (3-)) than for P-sufficient cultures (243 and 343 mg kg(-1) for 40 mg L(-1), respectively). Plants exposed to As(V) had uptake and accumulation values slightly higher than did plants exposed to As(III). No significant differences in bioaccumulation were found between plants exposed to a concentration of As(III) >1 mg L(-1) and those exposed to As(V) at the same concentration. This indicates a direct relationship to P content in the culture. Toxicity may result from the uptake of As(V) instead of PO(4) (3-) as a result of ion competition during uptake because of close thermodynamic properties, which may change the interaction among components in the media. The toxicity pattern is interpreted as a manifestation of changing speciation in the batch culture and of the oxidation of As(III) to As(V) in an oxygen-rich environment.

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Section: Relation Of Chemical Speciation To Toxicitymentioning

confidence: 90%

Toxicity of arsenic species to Lemna gibba L. and the influence of phosphate on arsenic bioavailability

Mkandawire

Lyubun

Kosterin

et al. 2004

Environmental Toxicology

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“…The results demonstrate that processes of plantmicrobe interactions may be very sensitive to HM toxicity and can be disturbed at metal concentrations below threshold toxicity levels determined for each partner separately. It was proposed previously that a high metal sensitivity of symbiosis between alfalfa and S. meliloti may be used as a test to assess the toxicity of soils contaminated with Cd and As (Wetzel and Werner 1995;Neumarnn et al 1998) It is likely that the same test can be created on the basis of symbiosis between pea and R. leguminosarum bv. viciae.…”

Section: Sensitivity Of Symbiosis Between Pea and Nodule Bacteria To mentioning

confidence: 98%

Role of Plant Genotype and Soil Conditions in Symbiotic Plant-Microbe Interactions for Adaptation of Plants to Cadmium-Polluted Soils

Belimov

Puhalsky

Safronova

et al. 2015

Water Air Soil Pollut

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We highlighted some of the key problems associated with the use of beneficial microorganisms for improving adaptation of plants to soils, polluted with heavy metals (HMs), especially Cd. Inoculation of pea line SGE and its Cd-tolerant mutant SGECd t with nodule bacteria Rhizobium leguminosarum bv. viciae demonstrated that nodulation process may be disturbed at Cd concentrations below threshold toxicity levels for each partner and the plant genotype plays a major role in nodulation under Cd stress. A comparative mathematical analysis of available information about Cd tolerance, accumulation of HMs (Cd, Cr, Cu, Ni, Pb, Sr and Zn), response to mycorrhizal fungus Glomus sp. and 15 phenotypic traits of 99 pea varieties revealed that (1) the Cd-sensitive varieties were more efficient in exploring the protective potential of symbiosis to compensate their deficit in Cd tolerance and (2) correlations between the studied traits exist and can be helpful for selection of plant-microbe systems adapted to polluted soils. In pot experiment with 11 varieties of Indian mustard, the plant growth-promoting effect of rhizobacterium Variovorax paradoxus 5C-2 negatively correlated with Cd tolerance and shoot Cd concentration of the plants grown in Cd-supplemented soil. In an outdoor pot experiment, inoculation of willow with the ectomycorrhizal fungus Pisolithus tinctorius and a cocktail of rhizobacteria stimulated root exudation, decreased soil pH and increased Cd mobilization in soil and Cd uptake by plants, but decreased plant growth at a moderate contamination level (25 mg Cd kg −1 ). Opposite effects were observed in highly contaminated soil (77 mg Cd kg −1 ). We propose a preliminary systematic framework of interactions between these factors that determine the success of microbial inoculation aimed at improving crop performance on HM-polluted soils or enhancing phytoremediation.

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“…The process of nitrogen fixation is sensitive to environmental cues, including the action of heavy metals (Angle et al, 1993;Neumann et al, 1998). Cadmium (Cd) is one of the most toxic elements and plants use different mechanisms to mitigate its toxic effects (Kulaeva and Tsyganov, 2011).…”

Section: Introductionmentioning

confidence: 99%

Efficacy of a Plant-Microbe System: Pisum sativum (L.) Cadmium-Tolerant Mutant and Rhizobium leguminosarum Strains, Expressing Pea Metallothionein Genes PsMT1 and PsMT2, for Cadmium Phytoremediation

et al. 2020

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Two transgenic strains of Rhizobium leguminosarum bv. viciae, 3841-PsMT1 and 3841-PsMT2, were obtained. These strains contain the genetic constructions nifH-PsMT1 and nifH-PsMT2 coding for two pea (Pisum sativum L.) metallothionein genes, PsMT1 and PsMT2, fused with the promoter region of the nifH gene. The ability of both transgenic strains to form nodules on roots of the pea wild-type SGE and the mutant SGECd t , which is characterized by increased tolerance to and accumulation of cadmium (Cd) in plants, was analyzed. Without Cd treatment, the wild type and mutant SGECd t inoculated with R. leguminosarum strains 3841, 3841-PsMT1, or 3841-PsMT2 were similar histologically and in their ultrastructural organization of nodules. Nodules of wild-type SGE inoculated with strain 3841 and exposed to 0.5 μM CdCl 2 were characterized by an enlarged senescence zone. It was in stark contrast to Cd-treated nodules of the mutant SGECd t that maintained their proper organization. Cadmium treatment of either wild-type SGE or mutant SGECd t did not cause significant alterations in histological organization of nodules formed by strains 3841-PsMT1 and 3841-PsMT2. Although some abnormalities were observed at the ultrastructural level, they were less pronounced in the nodules of strain 3841-PsMT1 than in those formed by 3841-PsMT2. Both transgenic strains also differed in their effects on pea plant growth and the Cd and nutrient contents in shoots. In our opinion, combination of Cd-tolerant mutant SGECd t and the strains 3841-PsMT1 or 3841-PsMT2 may be used as an original model for study of Cd tolerance mechanisms in legume-rhizobial symbiosis and possibilities for its application in phytoremediation or phytostabilization technologies.

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Toxicity testing of heavy metals with theRhizobium-legume symbiosis: High sensitivity to cadmium and arsenic compounds

Cited by 29 publications

References 12 publications

Toxicity of arsenic species to Lemna gibba L. and the influence of phosphate on arsenic bioavailability

Toxicity of arsenic species to Lemna gibba L. and the influence of phosphate on arsenic bioavailability

Role of Plant Genotype and Soil Conditions in Symbiotic Plant-Microbe Interactions for Adaptation of Plants to Cadmium-Polluted Soils

Efficacy of a Plant-Microbe System: Pisum sativum (L.) Cadmium-Tolerant Mutant and Rhizobium leguminosarum Strains, Expressing Pea Metallothionein Genes PsMT1 and PsMT2, for Cadmium Phytoremediation

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