Toxicity Test of Windu Shrimp (Penaeus monodon) Skin Chitosan With Brine Shrimp Lethality Test Method

Abstract

Chitosan is a modification of chitin compounds that are widely found in the outer skin of crustacean animals such as shrimp and crabs. This research includes isolation of chitin and chitosan: deproteination, demineralization, depigmentation and deacetylation namely transformation of chitin into chitosan, characterization of chitosan, FTIR, and chitosan toxicity test with five concentrations of test solution, namely 100 g/ml, 250 g/ml, 500 g/ml, 750 g/ml and 1000 g/ml using the BSLT method by looking at the number of deaths of Artemia salina L larvae (LC50). The results of tiger prawn shell chitosan (Penaeus monodon) obtained the % degree of deacetylation of 60%. The results of the toxicity test showed that chitosan was not toxic to Artemia salina Leach, indicated by the LC50 value > 1000µg/ml. chitosan windu 4994.16 g/ml, chitosan is not toxic

Isolation and Toxicity Test of Chitosan from Green Mussels (Perna viridis L.) With Brine Shrimp Lethality Test (BSLT) Method

“…Chitosan ventilated at 100-105°C for 2 hours, then cooled in a dexicator for 30 minutes and then weighed. This treatment carried out until the weight is constant [9].…”

Uji sitotoksisitas ekstrak etanol daun sirih cina (Peperomia pellucida [L.] Kunth) dengan metode brine Shrimp lethality test

“…Untuk membuat air laut buatan, larutkan 38 gram garam (tanpa yodium) ke dalam labu 1000 mL, tambahkan air hingga isi labu sesuai takaran, lalu aduk hingga adonan rata. Kemudian kertas Whatman digunakan untuk menyaring (Saragih et al, 2022).…”

Utilization Of Chitosan As A Natural Preservative Against Catfish

“…Chitosan is made from shrimp shells, crabs, crabs, snail shells, and so on. Several studies on the utilization of snail shells into chitosan obtained a degree of deacetylation of 65% (Ridwanto et al, 2016); on tiger shrimp shells the percentage of deacetylation was 60% (Saragih et al, 2022); on crab shells the degree of acetylation was 82.84% (Ridwanto et al, 2022); and 82.73% for the shells of the coral crab crab (Ridwanto et al, 2021).The content of chitin in shrimp shells is less than crab shells, but shrimp shells are easier to obtain and are available in large quantities. Currently, the processing of shrimp shell waste in Indonesia is still not optimal and is limited to a mixture of animal feed.…”