Toxicity screening of Diclofenac, Propranolol, Sertraline and Simvastatin using Danio rerio and Paracentrotus lividus embryo bioassays

Ribeiro, Sara; Torres, Tiago; Martíns, Rosário; Santos, Miguel M.

doi:10.1016/j.ecoenv.2015.01.008

Cited by 113 publications

(45 citation statements)

References 26 publications

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“…Larval abnormalities require a higher sample size and there are observer-dependent endpoints that may result in subjective microscopial inspection when considering the presence and the degree of the different abnormalities [32]. However, according to our results in Ribeiro et al, the classical endpoints reveal a higher sensitivity for the pharmaceuticals propranolol, simvastatin and sertraline [33]. This may be related to the bioactivity of pharmaceuticals or their behavior and mode of in non-target organisms.…”

Section: Discussionmentioning

confidence: 86%

“…For each chemical bioassay, initially six treatments conditions were set up: an experimental control, a solvent control (DMSO) and four experimental concentrations, for both species’ bioassay (eight replicates per treatment), placed in two equal-organized 24-well plates [33]. …”

Section: Methodsmentioning

confidence: 99%

“…Based on a set of characteristics present in zebrafish embryos at specific stages of development, three distinct periods were chosen to assess the effects of exposures: gastrula period (65%–75% epiboly stage), pharyngula period (prim 15–16) and larval stage (protruding-mouth), corresponding to 8, 32 and 80 h post fertilization (hpf), respectively [33,51]. Different parameters were recorded at each period of observation (Table 4).…”

Section: Methodsmentioning

confidence: 99%

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Screening the Toxicity of Selected Personal Care Products Using Embryo Bioassays: 4-MBC, Propylparaben and Triclocarban

Torres

Cunha

Martíns

et al. 2016

IJMS

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Recently, several emerging pollutants, including Personal Care Products (PCPs), have been detected in aquatic ecosystems, in the ng/L or µg/L range. Available toxicological data is limited, and, for certain PCPs, evidence indicates a potential risk for the environment. Hence, there is an urgent need to gather ecotoxicological data on PCPs as a proxy to improve risk assessment. Here, the toxicity of three different PCPs (4-Methylbenzylidene Camphor (4-MBC), propylparaben and triclocarban) was tested using embryo bioassays with Danio rerio (zebrafish) and Paracentrotus lividus (sea urchin). The No Observed Effect Concentration (NOEC) for triclocarban was 0.256 µg/L for sea urchin and 100 µg/L for zebrafish, whereas NOEC for 4-MBC was 0.32 µg/L for sea urchin and 50 µg/L for zebrafish. Both PCPs impacted embryo development at environmentally relevant concentrations. In comparison with triclocarban and 4-MBC, propylparaben was less toxic for both sea urchin (NOEC = 160 µg/L) and zebrafish (NOEC = 1000 µg/L). Overall, this study further demonstrates the sensitivity of embryo bioassays as a high-throughput approach for testing the toxicity of emerging pollutants.

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Section: Discussionmentioning

confidence: 86%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Screening the Toxicity of Selected Personal Care Products Using Embryo Bioassays: 4-MBC, Propylparaben and Triclocarban

Torres

Cunha

Martíns

et al. 2016

IJMS

Self Cite

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“…18,19 Existem vários relatos indicando que a fluoxetina e a sinvastatina têm efeitos tóxicos em ecossistemas aquáticos, causando o atraso no desenvolvimento de vários organismos aquáticos afetando diretamente a reprodução dos organismos. [20][21][22][23][24] De acordo com estudos realizados o principal risco ecológico de antibióticos está voltado à indução de bactérias resistentes, normalmente é considerado como o efeito adverso mais significativo de antibióticos. Em função de seu elevado consumo em todo o mundo, existem na literatura diversos estudos sobre os efeitos da amoxicilina em espécies aquáticas.…”

Section: Introductionunclassified

Photocatalitic Degradation of Sinvastatin, Amoxicillin and Fluoxetin Using TiO2 Immobilized in Cellulose Acetate

2017

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Beads of cellulose acetate containing titanium dioxide (TiO 2 /AC) were synthesized containing 10% and 6% (m/m) of TiO 2 and Ti, respectively. The TiO 2 is in the anatase form and is homogeneously dispersed on the surface of the cellulose acetate. The synthesized beads were efficient in the degradation of the micropollutants: Amoxicillin (99.0% in 240 minutes), Fluoxetine (85% in 180 minutes) and Simvastatin (99.1% in 30 minutes) by heterogeneous photocatalysis. The results obtained in our studies are similar or better than those obtained in other studies reported in the literature.Keywords: Beads of cellulose acetate; heterogeneous photocatalysis; organic micropollutants; advanced oxidative processes. ResumoEsferas de acetato de celulose contendo dióxido de titânio (TiO 2 /AC) foram sintetizadas contendo 10% e 6% (m/m) de TiO 2 e Ti, respectivamente. O TiO 2 encontra-se na forma anatase e está disperso homogeneamente sobre a superfície do acetato de celulose. As esferas sintetizadas foram eficientes na degradação dos micropoluentes: Amoxicilina (99,0% em 240 minutos), Fluoxetina (85% em 180 minutos) e Sinvastatina (99,1% em 30 minutos), através de fotocatálise heterogênea. Os resultados obtidos em nossos estudos são similares ou melhores dos que os obtidos em outros trabalhos relatados na literatura.Palavras-chave: Esferas de acetato de celulose; fotocatálise heterogênea; micropoluentes orgânicos; processos oxidativos avançados.

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“…Survey on effluent water in the Baltic sea runoff area have detected PROP in concentrations ranging from 0.01 to 0.03 µg/L [85] while in other water bodies, it had been found as high as 0.29 g/L [90]. PROP, when exposed to concentrations from 12.5 µg/L to 125 µg/L to zebra fish embryos and sea urchin embryos from 1.25 to 12.5 µg/L induced significant mortality rates and all concentrations above 5 µg/L resulted in statistically significant abnormality rate for both groups [91]. As mentioned in paper III, mode of action of PROP may be similar to an extent with both humans and mussels because of evolutionary conservation of molecular targets [90].…”

Section: Effects Of Propmentioning

confidence: 99%

Proteomics as a multifaceted tool in medicine and environmental assessment

Kuruvilla¹

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Proteomics is evolving as a multi-faceted tool for addressing various biochemical and biomedical queries in the field of scientific research. This involves various stages, ranging from sample preparation to data analysis and biological interpretation. Sample preparation involves isolating proteins from the sample source, purifying and digesting them to initiate shotgun proteomics. Shotgun proteomics identifies proteins by bottom-up proteomic approaches where proteins are identified from the fragmentation spectra of their own peptides.Paper I: deals with the simplification of functional characterization for nanoparticles intended for use in biomedicine. Proteomics was constructive in differentiating and semi-quantifying the surface of protein corona. This could be beneficial in predicting the interactions between nanoparticles and a biological entity like the cell or a receptor protein and provide initial valuable information related to targeting, uptake and safety.Paper II: deals with understanding effects of TiO2 nanoparticles on endothelial cells. A combinatorial approach, involving transcriptomics and proteomics was used to identify aberrations in the permeability and integrity of endothelial cells and tissues. Our study also investigated the correlation of size and how they motivated a differential cellular response. In case of intravenous entry for nanoparticles in targeted drug delivery systems, endothelial cells are the first barrier encountered by these drug carriers. This evaluation involving endothelial cell response could be very instrumental during the designing of NP based drug delivery systems.Paper III: Pharmaceuticals and its metabolites could be very hazardous, especially if its disposal is not managed properly. Since water bodies are the ultimate sink, these chemicals could end up there, culminating in toxicity and other 'mixture effects' in combination with other factors. To evaluate the effects of the pharmaceutical, propranolol and climatic factors like low salinity conditions, a microcosm exposure was designed and shotgun proteomics helped understand its impact on mussel gills. In this study too, a combination of transcriptomics and proteomics unveiled molecular mechanisms altered in response to stressors, both individually and in combination.Paper IV: An interplay of various factors like EBF1 and PAX5 determines B-cell lineage and commitment. This might have been materialized by direct and transient proteinprotein interactions. A unique method called BioID helped screen relevant interactions in living cells by the application of a promiscuous biotin ligase enzyme capable of tagging proteins through biotinylation based on a proximity radius. Biotinylation of endogenous proteins enabled their selective isolation by exploiting the high affinity of biotin and streptavidin on streptavidin coated agarose beads, leading to their identification by mass spectrometry. The biotinylated proteins were potential candidate interactors of EBF1 and PAX5, which were later confirmed by sequencing tec...

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Toxicity screening of Diclofenac, Propranolol, Sertraline and Simvastatin using Danio rerio and Paracentrotus lividus embryo bioassays

Cited by 113 publications

References 26 publications

Screening the Toxicity of Selected Personal Care Products Using Embryo Bioassays: 4-MBC, Propylparaben and Triclocarban

Screening the Toxicity of Selected Personal Care Products Using Embryo Bioassays: 4-MBC, Propylparaben and Triclocarban

Photocatalitic Degradation of Sinvastatin, Amoxicillin and Fluoxetin Using TiO2 Immobilized in Cellulose Acetate

Proteomics as a multifaceted tool in medicine and environmental assessment

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