Toxicity of two type II ribosome‐inactivating proteins (cinnamomin and ricin) to domestic silkworm larvae

Wei, Guoqing; Liu, Ren-Shui; Wang, Qiong; Liu, Wang-Yi

doi:10.1002/arch.20024

Cited by 33 publications

(15 citation statements)

References 23 publications

(19 reference statements)

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“…Several lines of evidence support the idea that ricin-B related lectins play a role in plant defense against pathogens (Chen et al, 2002b; Vandenbussche et al, 2004a,b) and insects (Wei et al, 2004; Shahidi-Noghabi et al, 2009). Over-expressing SNA-I′ or SNA-V from S. nigra in transgenic tobacco (Samsun NN) plants enhances the tobacco plant’s resistance against infection with tobacco mosaic virus.…”

Section: Pathogen Recognition Based On Protein–carbohydrate Interactionsmentioning

confidence: 77%

Lectin domains at the frontiers of plant defense

2014

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Plants are under constant attack from pathogens and herbivorous insects. To protect and defend themselves, plants evolved a multi-layered surveillance system, known as the innate immune system. Plants sense their encounters upon perception of conserved microbial structures and damage-associated patterns using cell-surface and intracellular immune receptors. Plant lectins and proteins with one or more lectin domains represent a major part of these receptors. The whole group of plant lectins comprises an elaborate collection of proteins capable of recognizing and interacting with specific carbohydrate structures, either originating from the invading organisms or from damaged plant cell wall structures. Due to the vast diversity in protein structures, carbohydrate recognition domains and glycan binding specificities, plant lectins constitute a very diverse protein superfamily. In the last decade, new types of nucleocytoplasmic plant lectins have been identified and characterized, in particular lectins expressed inside the nucleus and the cytoplasm of plant cells often as part of a specific plant response upon exposure to different stress factors or changing environmental conditions. In this review, we provide an overview on plant lectin motifs used in the constant battle against pathogens and predators during plant defenses.

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Section: Pathogen Recognition Based On Protein–carbohydrate Interactionsmentioning

confidence: 77%

Lectin domains at the frontiers of plant defense

2014

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“…Insecticidal activity of type-2 RIPs has been demonstrated towards some insects (Gatehouse et al 1990;Dowd et al 1998Dowd et al , 2003Wei et al 2004). Although the enzymatic mechanism of RIP activity is well defined, the physiological steps by which ribosome inactivation leads to cell death are not well understood.…”

Section: Introductionmentioning

confidence: 99%

Expression of Sambucus nigra agglutinin (SNA-I′) from elderberry bark in transgenic tobacco plants results in enhanced resistance to different insect species

2008

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Tobacco plants (Nicotiana tabacum cv Samsun NN) have been transformed with the gene encoding the type-2 ribosome-inactivating protein (RIP) SNA-I' from elderberry (Sambucus nigra) under the control of the Cauliflower Mosaic Virus 35S promoter. Previous research confirmed that these plants synthesize, correctly process and assemble a fully active RIP. Variability in protein expression was observed within the transgenic lines. The effects of the type-2 RIP SNA-I' delivered through a leaf feeding assay were evaluated in the laboratory on two economically important pest insects belonging to the orders of Hemiptera, the tobacco aphid (Myzus nicotianae) and Lepidoptera, the beet armyworm (Spodoptera exigua). In the experiment with aphids, significant effects were observed on the life parameters, such as survival, intrinsic rate of increase, net reproductive rate, mean generation time and mean daily offspring, whereas with caterpillars significant reduction in fresh weight as well as retardation in development were observed. In addition, significant increases in mortality were noted for insects fed on the transgenic lines as compared to wild type plants. This information provides further support for RIPs having a role in plant resistance to insect pest species

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“…Likewise, Bu et al (2008) showed that potato NAC domain proteins are induced rapidly and strongly after wounding but not systemically, and their transcript levels reach maximum after 1 h, consistent with our observations. Interestingly, JA is also reported to modulate expression patterns of ricin B lectins (type II ribosome inactivating proteins, RIPII) (Reinbothe et al 1994;Xu et al 2007) having negative impact on aphid growth (ShahidiNoghabi et al 2008(ShahidiNoghabi et al , 2009, shows toxicity to many other herbivores (Zhou et al 2000;Wei et al 2004;Van Damme 2008), and even reduces their rate of oviposition on seeds of chickpea (Sadeghi et al 2006). Our study shows that following 30 min of TMB feeding, RIPII transcript abundance increases more than fivefolds in the tolerant cultivar.…”

Section: Tmb Induces Unique Enrichment Of Specific Pathway Genes In Tmentioning

confidence: 94%

Molecular Landscape of Helopeltis theivora Induced Transcriptome and Defense Gene Expression in Tea

et al. 2014

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Tea is the second most consumed beverage worldwide whose cultivation is greatly challenged by a large variety of biotic and abiotic stresses. Among the biotic factors, the hemipteran pest Helopeltis theivora Waterhouse is particularly devastating in Asia and Africa, rendering the crop unsuitable for any downstream processing. The present study, for the first time, endeavors to dissect the molecular events associated with such infestations and identify potential molecular protagonists influencing Helopeltis tolerance in Assam tea. We analyzed the transcriptome of infested tea plants having contrasting responses to pest feeding by suppression subtractive hybridization and quantified the relative abundances of defense-associated transcripts. We analyzed 445 unigenes derived from 1558 expressed sequence tags (ESTs) from three different infestation conditions. Our study indicate that defense responses to the pest greatly vary in temporal and cultivar-specific manner characterized by an exclusive upregulation of specific defenseassociated genes in the tolerant cultivar. Moreover, it was observed that transcripts related to flavonoid biosynthesis, purine metabolism, formate metabolism, jasmonic acid biosynthesis and signaling, and cell wall metabolism are uniquely enriched in the same. Interestingly, those involved in caffeine biosynthesis remain comparatively low and unaltered. We report higher trichome densities on the abaxial surface of the leaf in tolerant cultivar while puncture causes their tender reorientation. SEM of the insect mouthparts reveal higher concentrations of sensilla-like structures near the distal end and tip of the proboscis which are known to act as chemoreceptors in other hemipterans. Our investigation provides the first ever glimpse of Helopeltis-induced defense gene network and provides the much needed platform for improvement of the crop for better Helopeltis tolerance through breeding and transgenic approaches. On a wider perspective, the study will augment our understanding of molecular responses to phloem herbivory in plants.

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Toxicity of two type II ribosome‐inactivating proteins (cinnamomin and ricin) to domestic silkworm larvae

Cited by 33 publications

References 23 publications

Lectin domains at the frontiers of plant defense

Lectin domains at the frontiers of plant defense

Expression of Sambucus nigra agglutinin (SNA-I′) from elderberry bark in transgenic tobacco plants results in enhanced resistance to different insect species

Molecular Landscape of Helopeltis theivora Induced Transcriptome and Defense Gene Expression in Tea

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