Toxicity of titanium dioxide nanoparticles on Pseudomonas putida

Combarros, R.G.; Collado, Sergio; Dı́az, Mario

doi:10.1016/j.watres.2015.12.040

Cited by 37 publications

(11 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although 93% of the total cells were still viable in suspensions amended with AgNPs of 5 mg L −1 , cell growth was eventually not displayed, implying that the increasing AgNPs dosage did not correspond to an increase in cell death ratio but accelerated the transition to VBNC status. Similar results were found when performing high concentrations (2 mg mL −1 ) of TiO 2 -NPs to Pseudomonas putida , that nearly all the cell population were viable but only one third of them were cultivable 27 . It has been previously reported that the VBNC state plays an critical role in the survival of bacteria, and in their ability to affect environment and human health 28 , 29 .…”

Section: Discussionsupporting

confidence: 80%

See 1 more Smart Citation

Aerobic condition enhances bacteriostatic effects of silver nanoparticles in aquatic environment: an antimicrobial study on Pseudomonas aeruginosa

Chen

Yang

Yuan

et al. 2017

Sci Rep

View full text Add to dashboard Cite

The intensive applications of silver nanoparticles (AgNPs) inevitably cause continuous release of such materials into environments, as a consequence posing potential risks to microbial communities in engineered or natural ecosystems. However, the magnitude of antibacterial capacity of nanoparticles is still inconclusive, owing to influential factors such as the size of nanoparticle, microbial species, or environmental conditions. To reveal whether the presence of air would alter AgNPs ecotoxicity, Pseudomonas aeruginosa PAO1, a facultative denitrifying bacterium and an opportunity pathogen, was used to study antibacterial assays under both anaerobic and aerobic conditions. The results indicate that the respiration status of P. aeruginosa affect the ecotoxicity of AgNPs. P. aeruginosa cultured under aerobic condition were more susceptible to AgNPs than that under anaerobic condition. Aerobic condition greatly enhanced bacteriostatic effects of AgNPs but not their bactericidal effects, as the ratio of viable but nonculturable (VBNC) bacteria remained above 90% when 5 mg L −1 AgNPs applied. Our findings offer further understanding for the degree of toxicity of nanoparticles on microbial ecosystems and underscore the importance of exposure condition (e.g. oxygen) in the mode of action of AgNPs.Silver nanoparticles (AgNPs) have been widely used in consumer products such as textile, cosmetics, and medical products, due to their excellent antibacterial, optical, and electronic properties [1][2][3] . AgNPs are the most popular nanoparticles in markets, which accounts for about 50% of commercial nanoproducts 4 . It is estimated that approximate 500 tons of AgNPs were produced each year worldwide 5,6 . As being incorporated in fabric and personal care products, the processes of fabrication and consumption thereby can release AgNPs from consumer products to sewage, eventually entering environment. Thus, it represents a potential risk to a great variety of organisms in natural or engineered ecosystems, including wastewater treatment plants (WWTPs).AgNPs have shown great antibacterial activities against various microbes. Choi et al. 7 reported that the respiration of autotrophic nitrifiers in a continuously stirred tank reactor was inhibited by 86% at 1 mg L −1 of AgNPs. Tan et al. 8 documented that AgNPs at 300 µg L −1 decreased nitrifying efficiency from 98% to 15%. In addition, Li et al. 9 showed that a commercial AgNPs at 10 mg L −1 was the miminum inhibitory concentration (MIC) to Escherichia coli, whereas a synthesized AgNPs exhibited 100% killing of E. coli when added at 4.5 mg L −110 . According to these previous studies 7-11 , the concentration of AgNPs that will cause toxic impacts on microbes, ranged from µg L −1 to mg L −1 . Such an inconclusive antibacterial capacity of nanoparticles may be associated with different exposure conditions or microorganism types, which may alter the antibacterial activity of nanoparticles. Some studies [12][13][14][15] suggested that the susceptibility of microbes, microbial adapt...

show abstract

Section: Discussionsupporting

confidence: 80%

“…As each cell in samples untercepts the laser beam, laser light is scattered in all derections. The FSC parameter was used as an indicator of cell size, whereas SSC parameter was used to indicate cell complexity 27 .…”

Section: Methodsmentioning

confidence: 99%

Aerobic condition enhances bacteriostatic effects of silver nanoparticles in aquatic environment: an antimicrobial study on Pseudomonas aeruginosa

Chen

Yang

Yuan

et al. 2017

Sci Rep

View full text Add to dashboard Cite

show abstract

“…A number of studies have revealed the toxicity effects of TiO 2 NPs on microorganisms, invertebrates, and higher plants (Menard et al, 2011). The toxicity assay using soil bacteria Pseudomonas putida and microalga Pseudokirchneriella subcapita demonstrated that TiO 2 NPs severely affect the growth and viability of these microorganisms (Aruoja et al, 2009; Combarros et al, 2016). A study indicated that TiO 2 toxicity in marine microalga, Nitzschia closterium , mainly arises from an increased reactive oxygen species (ROS) level (Xia et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Nanotitania Exposure Causes Alterations in Physiological, Nutritional and Stress Responses in Tomato (Solanum lycopersicum)

et al. 2017

View full text Add to dashboard Cite

Titanium dioxide nanoparticles (nanotitania: TiO2NPs) are used in a wide range of consumer products, paints, sunscreens, and cosmetics. The increased applications lead to the subsequent release of nanomaterials in environment that could affect the plant productivity. However, few studies have been performed to determine the overall effects of TiO2NPs on edible crops. We treated tomato plants with 0.5, 1, 2, and 4 g/L TiO2NPs in a hydroponic system for 2 weeks and examined physiological, biochemical, and molecular changes. The dual response was observed on growth and photosynthetic ability of plants depending on TiO2NPs concentrations. Low concentrations (0.5–2 g/L) of TiO2NPs boosted growth by approximately 50% and caused significant increase in photosynthetic parameters such as quantum yield, performance index, and total chlorophyll content as well as induced expression of PSI gene with respect to untreated plants. The high concentration (4 g/L) affected these parameters in negative manner. The catalase and peroxidase activities were also elevated in the exposed plants in a dose-dependent manner. Likewise, exposed plants exhibited increased expressions of glutathione synthase and glutathione S-transferase (nearly threefold increase in both roots and leaves), indicating a promising role of thiols in detoxification of TiO2NPs in tomato. The elemental analysis of tissues performed at 0.5, 1, and 2 g/L TiO2NPs indicates that TiO2NPs transport significantly affected the distribution of essential elements (P, S, Mg, and Fe) in roots and leaves displaying about threefold increases in P and 25% decrease in Fe contents. This study presents the mechanistic basis for the differential responses of titanium nanoparticles in tomato, and calls for a cautious approach for the application of nanomaterials in agriculture. GRAPHICAL ABSTRACTMovement of nanotitania in plant tissues.

show abstract

“…The accumulation of NPs of metals at high levels in the plant could not only impact their growth, but could pose a route for contamination of the food chain . The interaction mechanisms at the molecular level between nanoparticles and biological systems are largely unknown …”

Section: Introductionmentioning

confidence: 99%

Effect of ZnO Nanoparticles on Germination of Triticum aestivum Seeds

Awasthi

Bansal

Jangir

et al. 2017

Macromolecular Symposia

View full text Add to dashboard Cite

With the progression of nanotechnology, the use of nanoparticles in consumer products has increased dramatically. Because of their broad scale use, these nanomaterials can be expected to be present in the environment, raising concerns about their effects on the lives of plants and animals. Zinc oxide (ZnO), a nanomaterial, has specific surface area, high pore volume, low toxicity and long life‐span and thus is being used as promising material for cosmetics, antibacterial, chemical absorbents, polymer additives and catalysts. In this study ZnO nanoparticles were synthesized and characterized using various techniques like X‐ray Diffraction (XRD), Transmission Electron microscopy (TEM) and Ultra violet (UV) visible spectroscopy. Further, the effect of ZnO nanoparticles (NPs) was tested on wheat (Triticum aestivum) grown in hydroponics media. It was observed that ZnO NPs, at 50 mg/L have positive effect on seed germination, number of roots, plant biomass and overall growth of roots, shoots and leaves. These findings could be further applied to the agricultural fields to enhance the crop production.

show abstract

Toxicity of titanium dioxide nanoparticles on Pseudomonas putida

Cited by 37 publications

References 29 publications

Aerobic condition enhances bacteriostatic effects of silver nanoparticles in aquatic environment: an antimicrobial study on Pseudomonas aeruginosa

Aerobic condition enhances bacteriostatic effects of silver nanoparticles in aquatic environment: an antimicrobial study on Pseudomonas aeruginosa

Nanotitania Exposure Causes Alterations in Physiological, Nutritional and Stress Responses in Tomato (Solanum lycopersicum)

Effect of ZnO Nanoparticles on Germination of Triticum aestivum Seeds

Contact Info

Product

Resources

About