Toxicity of the organophosphate pesticide diazinon to crayfish of differing age

Buřič, Miloš; Kouba, Antonín; Máchová, Jana; Mahovská, I.; Kozák, Pavel

doi:10.1007/s13762-013-0185-4

Cited by 17 publications

(7 citation statements)

References 23 publications

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“…The remaining four taxa in the nine candidate bioindicators are all green algae in the family Scenedesmaceae. Congeners of these taxa have been previously found to co‐occur with crayfish, and have been shown to be sensitive to crayfish pesticides (Buřič et al, 2013; Sarιkaya et al, 2011), suggesting that they may constitute important habitat for P. clarkii . We note that invasive tilapia were also present in our study system, potentially inflating the number of taxa significantly associated with crayfish in the pre‐eradication samples.…”

Section: Discussionmentioning

confidence: 99%

Environmental DNA reveals invasive crayfish microbial associates and ecosystem‐wide biodiversity before and after eradication

et al. 2023

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Biodiversity monitoring in conservation projects is essential to understand environmental health, complexity, and recovery. However, traditional field surveys can be expensive, time‐consuming, biased toward visual detection, and/or only measure a limited set of taxa. Environmental DNA (eDNA) methods provide a new approach to biodiversity monitoring that has the potential to sample a taxonomically broader set of organisms with a similar effort, but many of these approaches are still in the early stages of development and testing. Here, we used multi‐locus eDNA metabarcoding to understand how the removal of invasive red swamp crayfish using cypermethrin pesticide impacts local biodiversity of a desert oasis ecosystem, as well as to detect crayfish both directly and indirectly. We tracked crayfish DNA signatures, microbial DNA associated with crayfish, and biodiversity of plant, fungal, animal, and bacterial communities through time. We were unsuccessful in detecting crayfish directly in either control tanks or oases using targeted metabarcoding primers for invertebrates and eukaryotes, similar to previous studies which have shown variable levels of success in detecting crayfish from environmental samples. However, we were successful in discerning a suite of 90 crayfish‐associated taxa to serve as candidate bioindicators of invasive presence using 16S and fungal ITS2 metabarcoding. Ranking these 90 taxa by their geographic distribution in eDNA surveys and by evidence of crayfish associations in the literature, we support nine taxa to be high ranking, and suggest they be prioritized in future biomonitoring. Biodiversity analyses from five metabarcode loci including plants, animals, and both prokaryotic and eukaryotic microbes showed that communities differed but that species richness remained relatively similar between oases through time. Our results reveal that, while there are limitations of eDNA approaches to detect crayfish and other invasive species, microbial bioindicators offer a largely untapped biomonitoring opportunity for invasive species management, adding a valuable resource to a conservation manager's toolkit.

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Section: Discussionmentioning

confidence: 99%

Environmental DNA reveals invasive crayfish microbial associates and ecosystem‐wide biodiversity before and after eradication

et al. 2023

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show abstract

“…The remaining four taxa in the 9 candidate bioindicators are all green algae in the family Scenedesmaceae. Congeners of these taxa have been previously found to co-occur with crayfish, and have been shown to be sensitive to crayfish pesticides (Buřič et al, 2013; Sarıkaya et al, 2011), suggesting that they may constitute important habitat for P. clarkii . We note that invasive tilapia were also present in our study system, potentially inflating the number of taxa significantly associated with crayfish in the pre-eradication samples.…”

Section: Discussionmentioning

confidence: 99%

“…Congeners of these taxa have been previously found to co-occur with crayfish, and have been shown to be sensitive to crayfish pesticides (Buřič et al, 2013;Sarιkaya et al, 2011), suggesting that they may constitute important habitat for P. clarkii.…”

Section: Candidate Bioindicators Of Crayfish Presence Are Valuable To...mentioning

confidence: 99%

Environmental DNA reveals invasive crayfish microbial associates and ecosystem-wide biodiversity before and after eradication

Ballare

Worth

Ruiz‐Ramos

et al. 2022

Preprint

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Biodiversity monitoring in conservation projects is essential to understand environmental status and recovery. However, traditional field surveys can be biased towards visual detection and/or focused on measuring the biodiversity of a limited set of taxa. Environmental DNA (eDNA) methods provide a new approach to biodiversity monitoring that has the potential to sample a taxonomically broader set of organisms with similar effort, but these approaches are still in the early stages of development and testing. Here, we explore the utility of multilocus eDNA metabarcoding to explore the impact on local biodiversity of removal of the red swamp crayfish, a globally invasive species, from a desert oasis ecosystem. We tracked crayfish DNA signatures, microbial DNA associated with crayfish, and biodiversity changes of plant, fungal, animal, and bacterial communities through time. We were unsuccessful in detecting crayfish in control tanks or oases using targeted metabarcoding primers for invertebrates and eukaryotes. Metabarcoding of the 16S (targeting prokaryotes) and the ITS1 (targeting fungi) loci in the invaded oasis and tanks pre-removal were, however, successful in discerning a suite of 90 crayfish-associated taxa to serve as candidate bioindicators of invasive presence. Ranking these 90 taxa by their geographic distribution in eDNA surveys and by evidence of crayfish-associations in the literature, we support 9 taxa to be high-ranking, and suggest they be prioritized in future biomonitoring. Biodiversity analyses from five metabarcode loci including plants, animals, and both prokaryotic and eukaryotic microbes showed that communities differed but that species richness remained relatively similar between oases through time. Our results reveal that, while there are limitations of eDNA approaches to detect crayfish and other invasive species, microbial bioindicators offer a largely untapped biomonitoring opportunity for invasive species management, adding a valuable resource to a conservation manager’s toolkit.

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“…In contrast to cavefishes, we found that cave crayfishes had a strong negative relationship with human disturbance. Cave crayfishes may be more influenced than cavefishes by humaninduced landscape changes because of pesticide applications in urban and agricultural settings that specifically www.nature.com/scientificreports/ target arthropods 78,79 . Follow-up testing of the chemical constituents present in runoff may be warranted to better understand mechanisms on species persistence.…”

Section: Discussionmentioning

confidence: 99%

Lithology and disturbance drive cavefish and cave crayfish occurrence in the Ozark Highlands ecoregion

Mouser

Brewer

Niemiller

et al. 2022

Sci Rep

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Diverse communities of groundwater-dwelling organisms (i.e., stygobionts) are important for human wellbeing; however, we lack an understanding of the factors driving their distributions, making it difficult to protect many at-risk species. Therefore, our study objective was to determine the landscape factors related to the occurrence of cavefishes and cave crayfishes in the Ozark Highlands ecoregion, USA. We sampled cavefishes and cave crayfishes at 61 sampling units using both visual and environmental DNA surveys. We then modeled occurrence probability in relation to lithology and human disturbance while accounting for imperfect detection. Our results indicated that occurrence probability of cave crayfishes was negatively associated with human disturbance, whereas there was a weak positive relationship between cavefish occurrence and disturbance. Both cavefishes and cave crayfishes were more likely to occur in limestone rather than dolostone lithology. Our results indicate structuring factors are related to the distribution of these taxa, but with human disturbance as a prevalent modifier of distributions for cave crayfishes. Limiting human alteration near karst features may be warranted to promote the persistence of some stygobionts. Moreover, our results indicate current sampling efforts are inadequate to detect cryptic species; therefore, expanding sampling may be needed to develop effective conservation actions.

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Toxicity of the organophosphate pesticide diazinon to crayfish of differing age

Cited by 17 publications

References 23 publications

Environmental DNA reveals invasive crayfish microbial associates and ecosystem‐wide biodiversity before and after eradication

Environmental DNA reveals invasive crayfish microbial associates and ecosystem‐wide biodiversity before and after eradication

Environmental DNA reveals invasive crayfish microbial associates and ecosystem-wide biodiversity before and after eradication

Lithology and disturbance drive cavefish and cave crayfish occurrence in the Ozark Highlands ecoregion

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