Toxicity of surface-modified PLGA nanoparticles toward lung alveolar epithelial cells

Grabowski, Nadège; Hillaireau, Hervé; Vergnaud-Gauduchon, Juliette; Santiago, Leandro; Kerdine-Römer, Saadia; Pallardy, Marc; Tsapis, Nicolas; Fattal, Elias

doi:10.1016/j.ijpharm.2013.05.025

Cited by 104 publications

(66 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although PLGA has been widely described to be biocompatible and biodegradable (Makadia et al, 2011), there are studies reporting some cell toxicity in culture (Grabowski et al, 2013). Thus, we first evaluated if PLGA MPs could affect neurite outgrowth by loading FITC-BSA in the MPs and embedding them within the collagen matrix surrounding DRG and SC cultures.…”

Section: In Vitro Effects Of Ntf Loaded Mp On Neurite Outgrowthmentioning

confidence: 99%

Focal release of neurotrophic factors by biodegradable microspheres enhance motor and sensory axonal regeneration in vitro and in vivo

et al. 2016

View full text Add to dashboard Cite

Neurotrophic factors (NTFs) promote nerve regeneration and neuronal survival after peripheral nerve injury. However, drawbacks related with administration and bioactivity during long periods limit their therapeutic application. In this study, PLGA microspheres (MPs) were used to locally release different NTFs and evaluate whether they accelerate axonal regeneration in comparison with free NTFs or controls. ELISA, SEM, UV/visible light microscopy, organotypic cultures of DRG explants and spinal cord slices were used to characterize MP properties and the bioactivity of the released NTFs. Results of organotypic cultures showed that encapsulated NTFs maintain longer bioactivity and enhance neurite regeneration of both sensory and motor neurons compared with free NTFs. For in vivo assays, the rat sciatic nerve was transected and repaired with a silicone tube filled with collagen gel or collagen mixed with PBS encapsulated MPs (control groups) and with free or encapsulated NGF, BDNF, GDNF or FGF-2. After 20 days, a retrotracer was applied to the regenerated nerve to quantify motor and sensory axonal regeneration. NTF encapsulation in MPs improved regeneration of both motor and sensory axons, as evidenced by increased numbers of retrolabeled neurons. Hence, our results show that slow release of NTFs with PLGA MP enhance nerve regeneration.

show abstract

Section: In Vitro Effects Of Ntf Loaded Mp On Neurite Outgrowthmentioning

confidence: 99%

Focal release of neurotrophic factors by biodegradable microspheres enhance motor and sensory axonal regeneration in vitro and in vivo

et al. 2016

View full text Add to dashboard Cite

show abstract

“…20 L929, a normal cell line, has been widely used in a series of studies including drug delivery to the inner ear. 21,22 As shown in Figure 1A, the viability of L929 cells remained high after being treated with the four types of NPs at various concentrations, even up to 25 mg/mL (the original NPs suspension), suggesting that PLGA-based …”

Section: Resultsmentioning

confidence: 99%

Nanomedicine strategy for optimizing delivery to outer hair cells by surface-modified poly(lactic/glycolic acid) nanoparticles with hydrophilic molecules

Wen¹,

Ding²,

Cai³

et al. 2016

IJN

View full text Add to dashboard Cite

“…Cationic nanoparticles are conceivably incorporated into lipid rafts in plasma membrane, 30 which are microdomains in the cell membrane with a high level of glycosphingolipids and protein receptors. Previous studies 31,32 indicate the adhesion of lipid and PLGA nanoparticles onto lipid rafts of the cell surface. Exposure of cationic nanoparticles thus compromised the membrane integrity, which allowed LDH elevation and the subsequent release of cellular contents due to leakage.…”

Section: •-mentioning

confidence: 98%

Cationic additives in nanosystems activate cytotoxicity and inflammatory response of human neutrophils: lipid nanoparticles versus polymeric nanoparticles

Hwang¹,

Aljuffali²,

Lin³

et al. 2015

IJN

View full text Add to dashboard Cite

This report compares the effect of lipid and polymeric nanoparticles upon human neutrophils in the presence of cationic surfactants. Nanostructured lipid carriers and poly(lactic-co-glycolic) acid nanoparticles were manufactured as lipid and polymeric systems, respectively. Some cytotoxic and proinflammatory mediators such as lactate dehydrogenase (LDH), elastase, O 2 •− , and intracellular Ca 2+ were examined. The nanoparticles showed a size of 170–225 nm. Incorporation of cetyltrimethylammonium bromide or soyaethyl morpholinium ethosulfate, the cationic surfactant, converted zeta potential from a negative to a positive charge. Nanoparticles without cationic surfactants revealed a negligible change on immune and inflammatory responses. Cationic surfactants in both nanoparticulate and free forms induced cell death and the release of mediators. Lipid nanoparticles generally demonstrated a greater response compared to polymeric nanoparticles. The neutrophil morphology observed by electron microscopy confirmed this trend. Cetyltrimethylammonium bromide as the coating material showed more significant activation of neutrophils than soyaethyl morpholinium ethosulfate. Confocal microscope imaging displayed a limited internalization of nanoparticles into neutrophils. It is proposed that cationic nanoparticles interact with the cell membrane, triggering membrane disruption and the following Ca 2+ influx. The elevation of intracellular Ca 2+ induces degranulation and oxidative stress. The consequence of these effects is cytotoxicity and cell death. Caution should be taken when selecting feasible nanoparticulate formulations and cationic additives for consideration of applicability and toxicity.

show abstract

Toxicity of surface-modified PLGA nanoparticles toward lung alveolar epithelial cells

Cited by 104 publications

References 36 publications

Focal release of neurotrophic factors by biodegradable microspheres enhance motor and sensory axonal regeneration in vitro and in vivo

Focal release of neurotrophic factors by biodegradable microspheres enhance motor and sensory axonal regeneration in vitro and in vivo

Nanomedicine strategy for optimizing delivery to outer hair cells by surface-modified poly(lactic/glycolic acid) nanoparticles with hydrophilic molecules

Cationic additives in nanosystems activate cytotoxicity and inflammatory response of human neutrophils: lipid nanoparticles versus polymeric nanoparticles

Contact Info

Product

Resources

About