Toxicity of Staphylococcal Toxic Shock Syndrome Toxin 1 for Germ-Free and Conventional Piglets

Bulanda, Małgorzata; Zaleska, Małgorzata; Mandel, L.; Talafantová, M.; Trávníček, J.; Kunstmann, G.; Mauff, G.; Pulverer, G.; Heczko, Piotr B

doi:10.1093/clinids/11.supplement_1.s248

Cited by 12 publications

(15 citation statements)

References 9 publications

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“…Several authors used rabbits and monkeys as well as germ-free animals as experimental model, testing different inoculation methods. Bulanda et al (1989) compared the effects of TSST-1 in conventional and germ-free animals. Rabbits and piglets developed similar reactions, but the authors observed that germfree animals are less sensitive to toxin action than conventional animals.…”

Section: Introductionmentioning

confidence: 99%

The use of mice as animal model for testing acute toxicity (LD-50) of toxic shock syndrome toxin

Dias

Carmo

Heneine

et al. 2009

Arq. Bras. Med. Vet. Zootec.

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Acute toxicity test (LD-50) using toxic shock syndrome toxin (TSST-1) was tested in BALB/c, C57BL/6 and Swiss mice. Animals (n = 10) were intraperitoneally injected with TSST-1 (0.01-10.0µg/mouse) followed 4h later by potentiating dose of lipopolysaccharide (75.0µg of LPS -E. coli O111:B4) and cumulative mortality was recorded over 72h. Control animals received either TSST-1 or LPS alone. The data were submitted to quiSquare test and acute toxicity test was calculated by probit analysis (confidence limits expressed as µg toxin/kg). BALB/c mice was the most sensitive (20.0µg/kg, 95% confidence limits: 9.0-92.0) followed by C57BL/6 (38.5µg/kg, 95% confidence limits: 9.11-401.6). Data from Swiss mice was not conclusive, indicating only low sensitivity. Selection of the animal model and standardization of the experiment are fundamental for the development of serum neutralization tests used for final quality control of vaccine production.Keywords: mice, TSST-1, acute toxicity (LD-50), Staphylococcus 0), seguida da C57BL/6 (38,6 RESUMO A toxicidade aguda (DL-50) da toxina da síndrome do choque tóxico (TSST-1) foi testada em linhagens de camundongos BALB/c, C57BL/6 e Suíça. Os animais (n=10) inoculados intraperitoneal com doses crescentes de toxina (0,01 -10,0µg/animal) receberam 4h após 75µg de LPS (E. coli O111: B4). A toxicidade aguda (DL-50) foi observada por um período de 72h e os dados submetidos ao teste de qui-quadrado. Os resultados e os limites de confiança foram expressos em µg de toxina/kg. A linhagem BALB/c apresentou maior sensibilidade

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Section: Introductionmentioning

confidence: 99%

The use of mice as animal model for testing acute toxicity (LD-50) of toxic shock syndrome toxin

Dias

Carmo

Heneine

et al. 2009

Arq. Bras. Med. Vet. Zootec.

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“…Similar results have been reported in a recent study in miniature pigs. 20 These results suggest that the immediate role of TSST-1 in the pathogenesis of Gram-positive bacterial sepsis is to induce cardiac and respiratory failure rather than primary refractory systemic hypotension. Accordingly, we have focused on the initial stage of the biological process induced by continuous infusion of TSST-1.…”

Section: Discussionmentioning

confidence: 86%

“…Hence, pigs were selected as the model animals since pigs have been shown to be sensitive to TSST-1 toxicity 20,23,24 and are also of sufficient size to withstand the multiple sampling required. In the present study, conventional pigs were treated with TSST-1 alone by continuous infusion in an effort to develop a model for the superantigen-induced multiple organ dysfunction observed in patients with Gram-positive bacterial sepsis.…”

Section: Discussionmentioning

confidence: 99%

“…These results are consistent with the report by Bulanda and coworkers. 20 To evaluate the sensitivity of pigs to TSST-1, LPS lethality enhancement capacity 19 was examined in the TSST-1 infused pigs. LPS was infused 1 h after TSST-1 infusion terminated, at a concentration of 10 mg/kg/h for 1 h. This LPS concentration was of non-lethal level; however, when pigs were pre-treated with TSST-1 at the same dose in the experiment, all animals died within 24 h (data not shown).…”

Section: Discussionmentioning

confidence: 99%

“…15 Rabbits 18 and pigs 20 are highly susceptible species, while mice 21 and monkeys 22 do not exhibit any significant TSS-like symptoms. Accordingly, rabbits are the most widely used model animals for Gram-positive bacterial sepsis.…”

Section: Introductionmentioning

confidence: 99%

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Toxicity of Staphylococcal Toxic Shock Syndrome Toxin 1 for Germ-Free and Conventional Piglets

Cited by 12 publications

References 9 publications

The use of mice as animal model for testing acute toxicity (LD-50) of toxic shock syndrome toxin

The use of mice as animal model for testing acute toxicity (LD-50) of toxic shock syndrome toxin

Superantigen-induced multiple organ dysfunction in a toxin-concentration-controlled and sequential parameter-monitored swine sepsis model

Porcine Vagina Ex Vivo as a Model for Studying Permeability and Pathogenesis in Mucosa

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