Toxicity of silver nanoparticles to rainbow trout: A toxicogenomic approach

Gagné, François; André, C.; Skirrow, Rachel C.; Gélinas, Malorie; Auclair, J. L.; Aggelen, Graham van; Turcotte, Patrice; Gagnon, Christian

doi:10.1016/j.chemosphere.2012.05.063

Cited by 87 publications

(39 citation statements)

References 20 publications

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“…This observation was in agreement with the AgNP mean true diameter sizes measured with DLS in the three waters (90 nm, 40 nm and 34 nm for tap, green, and brown waters respectively). This finding confirms the observation of Gagne et al [9], who indicated that AgNPs spontaneously form aggregates in aquarium water during natural conditions exposure. This aggregation could be due to the organic matter composed mainly by fluvic and humic acids [36,37].…”

Section: Discussionsupporting

confidence: 82%

“…+ can induce modifications of enzymatic activities in the liver [9], induced cellular lipid peroxidation [45], inflammationand reduced the total glutathione [46]. In presence of AgNPs, significant increase in LPO activity was observed in fish liver in brown water and confirm the oxyradical formation and their damages to the liver [47,48].…”

Section: Agmentioning

confidence: 99%

“…For example, fish exposed to silver NPs led to embryonic deformation and lethality, inflammatory effects, dampened mitochondrial activity, oxidative stress mechanism, cytotoxicity, and apoptosis [4][5][6][7][8]. AgNPs change gene expression in the rainbow trout liver with the increase of gene expression in genes involved in oxidative stress (lipid peroxidation, oxidized metallothioneins), and the immune function [9]. However the behavior, bioavailability and toxicity of AgNPs in various types of freshwater are less understood at the present time.…”

Section: Introductionmentioning

confidence: 99%

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Fate and Immunotoxic Effects of Silver Nanoparticles on Rainbow Trout in Natural Waters

P¹

2015

J Nanomed Nanotechnol

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This study investigates the fate and the effects of silver nanoparticles (AgNPs) in rainbow trout (Oncorhynchus mykiss). Juveniles were exposed to 50 µg/L of AgNPs 20 nm and 1 µg/L of dissolved silver Ag + (as AgNO 3 ) in three types of water differing in conductivity, pH and organic matter: green water (Saint-Lawrence River), brown water (Ottawa River), and UV-treated and dechlorinated tap water. After a 96 h exposure, chemical analyses were performed on both water samples and fish tissues (liver and gills) and a suite of immune and hepatic biomarkers were also performed on fish. Results demonstrated that brown water with high organic carbon values (7 mg/L), promoted the formation of small AgNP aggregates that were bioaccumulated in fish gills and liver. In fish exposed to AgNPs, immunostimulation and hepatic oxidative stress were observed in pronephros and liver respectively. Dissolved Ag + in brown water was associated to small colloids, promoted silver bioavailability and induced leucocytes immunostimulation. The higher alkalinity and conductivity of green water promoted morphological AgNPs transformations. Although, AgNPs neither induced immune nor hepatic damages, AgNO 3 induced leucocyte stimulation and a hepatic pro-inflammatory response. Ag from AgNPs and AgNO 3 was bioaccumulated in gills, and liver without hepatic injury (oxidative stress). AgNO 3 induced immunosuppression and oxidative stress in pronephros. This study shows that water parameters influence the fate, bioavailability and the toxicity AgNPs and dissolved Ag + in fish. Brown water showed more harmful effects with AgNPs than tap and green waters.

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Section: Discussionsupporting

confidence: 82%

Section: Agmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Fate and Immunotoxic Effects of Silver Nanoparticles on Rainbow Trout in Natural Waters

P¹

2015

J Nanomed Nanotechnol

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“…Gagné et al (2012) showed that citrate-stabilized AgNPs (0.06-6 g/L) accumulated in the liver of rainbow trout after a 4-day exposure, suggesting that some of these particles pass through the gills into the blood and accumulate in the liver. Moreover, Farmen et al (2012) reported that AgNPs (1-100 g/L; unspecified coating) accumulated in the gills of Atlantic salmon (Salmo salar) after a 48 h exposure period; AgNPs also induced the expression of stress and metal detoxification genes and suppressed the mRNA levels of Na + /K + -ATPase.…”

Section: Toxicity Of Agnps In Fishmentioning

confidence: 98%

Predicting the environmental impact of nanosilver

Massarsky

Trudeau

Moon

2014

Environmental Toxicology and Pharmacology

126

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“…After prolonged exposures to relatively high concentrations, low cytotoxicity has been seen in cultured cells owing to increases in oxidative stress, most likely due to the increase of antioxidant defenses (Thakor et al, 2011). Recently, Gagné et al (2012) performed a DNA microarray study of rainbow trout (Oncorhynchus mykiss) in order to identify the fundamental mode of action of AgNPs using the gene expression level. They found that 31% of the genes (from 270 studied genes) responded to either nano-or dissolved-silver, while 9.6% were specifically affected by AgNPs.…”

Section: Discussionmentioning

confidence: 99%

Lipid Peroxidation and Protein Oxidation Induced by Different Nanoparticles in Zebrafish Organs

2015

AEER

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Abstract. The protein oxidation and the lipid peroxidation induced by the presence of five different kinds of nanoparticles were determined in different tissues of zebrafish (Danio rerio). Both oxidative stress markers showed to be dose-dependent. At 1 ppm nanoparticle concentration, the five assayed nanoparticles showed higher protein oxidation than the control experiments. Europium-doped yttrium oxide (Y 2 O 3 :Eu) and carboxylated multi-walled carbon nanotubes (MWCNT-COOH) showed the highest effect, especially in ovaries, testicles and brain, reaching carbonyl content in proteins up to 4-times higher than control, while bismuth germanate (BGO) and MWCNT-COOH nanoparticles induced lipid peroxidation in all analyzed tissues, reaching values up to two-times higher than those from non-exposed control fish. BGO and silver nanoparticles (AgNP) showed less oxidative effect; nevertheless, the protein oxidation still accounted for up to 3-times the control fish values. At 1 ppm, the protein oxidation induced by some nanoparticles reached the values obtained in the experiments with 100 μM H 2 O 2 . On the other hand, no significant differences were found in gene expression of oxidative stress markers after 48 h treatment. The results from this work clearly indicate that exposure to those nanoparticles may cause significant protein and lipid oxidative damage.

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Toxicity of silver nanoparticles to rainbow trout: A toxicogenomic approach

Cited by 87 publications

References 20 publications

Fate and Immunotoxic Effects of Silver Nanoparticles on Rainbow Trout in Natural Waters

Fate and Immunotoxic Effects of Silver Nanoparticles on Rainbow Trout in Natural Waters

Predicting the environmental impact of nanosilver

Lipid Peroxidation and Protein Oxidation Induced by Different Nanoparticles in Zebrafish Organs

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