Toxicity of redLiriope platyphyllamanufactured by steaming process on liver and kidney organs of ICR mice

Abstract

Red Liriope platyphylla (RLP) produced by steaming process has been reported to enhance the secretion of insulin and nerve growth factor (NGF). However, there has been no report on the toxicity of RLP in the specific organs of mice. To investigate the toxic effect of RLP, we tried to observe a significant alteration on body weight, food/water intake, organ weight, liver pathology and kidney pathology in female ICR mice received 12.5, 25.0 and 50.0 mg/kg body weight/day of RLP via gavage for 10 days. Out of seven organs including brain, heart, lung, liver, kidney, spleen and ovary, two organs (heart and lung) showed significantly decreased weights in the medium dosage RLP-treated group, whereas weights of other organs were maintained at constant levels in all dosage groups. In the liver toxicity analysis, no significant increase of alkaline phosphatase (ALP), alanine aminotransferase (ALT), and aspartate amino-transferase (AST) were detected in any RLP-treated group compared to vehicle-treated group. The specific pathological changes induced by most of toxic compounds were not observed in the liver in microscopic examination. Furthermore, in the kidney toxicological analysis, a significant enhancement of the blood urea nitrogen (BUN) concentration was detected in the high dosage RLP-treated group compared to the vehicle-treated group. However, the serum creatinine (CA) concentration on the serum biochemistry as well as the pathological changes in microscopic examination were not significantly different between the vehicle- and RLP-treated groups. Therefore, these results suggest that RLP does not induce any specific toxicity in liver or kidney tissues of mice, although the BUN level slightly increased in 50.0 mg/kg of RLP-treated group.

Phytochemical and pharmacological overview on <i>Liriopes radix</i>

“…However, a significant enhancement of the blood urea nitrogen (BUN) was detected in the 100 mg/kg dosage. Therefore, these results suggest that L. platyphylla does not induce any specific toxicity in liver and kidney tissues of mice [10].…”

Lipophilicity Determines Routes of Uptake and Clearance, and Toxicity of an Alpha-Particle-Emitting Peptide Receptor Radiotherapy

“…Serum specimens were tested for BUN and creatinine levels as indicators of renal toxicity, and ALKP, ALT, and AST levels as indicators of hepatic toxicity (Table S4). Elevated BUN levels were observed in some mice injected with 225 Ac-DOTA-MC1RL, 225 Ac-DOTA-Ahx-MC1RL, 225 Ac-DOTA-DLDL-MC1RL, and 225 Ac-DOTA-DLDG-MC1RL at ≥193.6, 200.1, 11.5, and 19.36 kBq of 225 Ac activity, respectively.…”

Anti-Inflammatory and Antioxidant Activities of Lipophilic Fraction from Liriope platyphylla Seeds Using Network Pharmacology, Molecular Docking, and In Vitro Experiments

“…Additionally, a double-blinded randomized placebo-controlled trial has shown that L. platyphylla extract, administered at a dosage of 1000 mg/day for four weeks, improves respiration functions in heathy volunteers without any observed adverse effects, confirming its safety and nontoxicity in use [10]. Red L. platyphylla, produced through a steaming process, dose not exhibit significant toxic effects in a mouse model [11]. Furthermore, its health benefits are ascribed to bioactive compounds, such as spirostanol saponins (spicatoside A and D), homoisoflavonoid, benzofurans, and sesquiterpenoids [12,13].…”