Toxicity of raw and processed roots of Polygonum multiflorum

Wu, Xiaoqing; Chen, Xiaozhen; Huang, Qingchun; Fang, Dong‐Mei; Li, Guo‐You; Zhang, Guolin

doi:10.1016/j.fitote.2011.12.012

Cited by 93 publications

(70 citation statements)

References 12 publications

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“…PM hepatotoxicity is of great concern; however, the components that induce hepatotoxicity are unknown. Besides, the present studies also have many contradictions, such as some studies demonstrated that PMW has greater toxicity than other extracts [29,30], but another study considered as PME [31]. In this assays, it is proved that the water extractions were less toxic than the 70% ethanol extractions which indicated that water decoction was a more rational extractive agent for PM and PMP.…”

Section: Combined Results Of the Toxicity And Data Analysis To Identimentioning

confidence: 68%

A novel method to analyze hepatotoxic components in Polygonum multiflorum using ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry

Lin

Zhang

et al. 2015

Journal of Hazardous Materials

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Section: Combined Results Of the Toxicity And Data Analysis To Identimentioning

confidence: 68%

A novel method to analyze hepatotoxic components in Polygonum multiflorum using ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry

Lin

Zhang

et al. 2015

Journal of Hazardous Materials

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“…Moreover, it has been revealed to cause in vivo hepatotoxicity. 9,10 Using well-designed in vitro assays is now an efficient approach for predicting the toxicity of chemicals to humans, which facilitates understanding the mechanism of the mode of action. In previous studies, the cytotoxicity of chemicals such as perfluorooctanoic acid and pyrrolizidine has been researched in human liver cells.…”

Section: Introductionmentioning

confidence: 99%

Metabolomic profiling of emodin-induced cytotoxicity in human liver cells and mechanistic study

Liu

et al. 2015

Toxicol. Res.

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Emodin is one of the most representative natural anthraquinone polyphenols and the liver is one of the major target organs for drug-induced toxicology. The hepatocyte is frequently affected due to its role in emodin metabolism and accumulation. Although the hepatotoxicity of emodin has been reported, its toxicological mechanism is still unclear. The purpose of the present study was to evaluate the cytotoxicity of emodin in cultured human normal liver cells (L-02), to investigate the toxicity-related metabolic pathways and to predict the possible toxicity mechanism. Cell viability was analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Cytotoxicity tests demonstrated a concentration-dependent toxic effect of emodin on L-02 cells. Cells were treated for 48 h with low, medium and high doses of emodin, respectively, and then subjected to metabolomics analysis using ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS). Intracellular metabolomics analysis revealed that emodin significantly disturbed cellular glutathione and fatty acid metabolism. In addition, an emodin-cysteine adduct was identified in cell culture medium, and its level increased with increasing concentrations of emodin. The possible relationship among metabolic disorders, adduct formation and emodin hepatotoxicity was also discussed. This study provides new insight into the cytotoxicity of emodin on metabolic pathways in human liver cells.

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“…Some studies have suggested that THSG is a toxic component of PMR (Wu et al, 2012; Meng et al, 2017), in which case, less THSG is safer for human consumption. Our results showed that the content of trans -THSG gradually decreased after each processing cycle and was lowest after nine cycles, while the content of cis -THSG initially increased and only began decreasing after the fifth processing cycle, reaching its lowest content after nine cycles.…”

Section: Resultsmentioning

confidence: 99%

Integrating Targeted and Untargeted Metabolomics to Investigate the Processing Chemistry of Polygoni Multiflori Radix

Liang

Zhou

et al. 2018

Front. Pharmacol.

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Polygoni Multiflori Radix (PMR, Heshouwu in Chinese), derived from the tuberous roots of Polygonum multiflorum Thunb., is a widely-used Chinese medicinal material. For traditional clinical use, raw PMR (RPMR) is processed by nine cycles of steaming and drying to generate processed PMR (PPMR); RPMR and PPMR have distinct medicinal purposes based on the theory of traditional Chinese medicine. While PMR has been processed for hundreds of years, including the present, the chemistry of that processing has not been well studied. In this study, targeted and untargeted metabolomics analyses using ultra-performance liquid chromatography-quadrupole/time-of-flight mass spectrometry (UPLC-QTOF-MS/MS) and ultra-performance liquid chromatography-quadrupole/triple quadrupole mass spectrometry (UPLC-QqQ-MS/MS) were integrated to investigate the processing chemistry of PMR. The results demonstrate that processing by nine cycles of steaming and drying qualitatively and quantitatively alters the chemical profile of PMR. Several mechanisms, namely hydrolysis, dehydration, isomerization, and Maillard reaction appear to be involved in the chemical transformation that occurs. The qualitative and quantitative data further suggest that nine cycles might be necessary for the preparation of PPMR, as PPMR that has been processed nine times shows significant differences in its chemical profile.

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Toxicity of raw and processed roots of Polygonum multiflorum

Cited by 93 publications

References 12 publications

A novel method to analyze hepatotoxic components in Polygonum multiflorum using ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry

A novel method to analyze hepatotoxic components in Polygonum multiflorum using ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry

Metabolomic profiling of emodin-induced cytotoxicity in human liver cells and mechanistic study

Integrating Targeted and Untargeted Metabolomics to Investigate the Processing Chemistry of Polygoni Multiflori Radix

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