Toxicity of PVA-stabilized silver nanoparticles to algae and microcrustaceans

Becaro, Aline A.; Jonsson, Cláudio Martín; Puti, Fernanda C.; Siqueira, Maria Célia; Mattoso, Luiz H. C.; Corrêa, Daniel S.; Ferreira, Marcos D.

doi:10.1016/j.enmm.2014.11.002

Cited by 54 publications

(31 citation statements)

References 44 publications

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“…AgNP can be synthesized from AgNO 3 by different methods such as using reducing agents like citrate or NaBH 4 with further stabilization of the nanoparticle with compounds such as polyvinyl alcohol (PVA) [13][14][15] . A citrate-based reduction is most commonly used in the synthesis of AgNP because of its reducing and stabilizing functionality.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of silver nanoparticle encapsulation in DPPC-based liposome by different methods for enhanced cytotoxicity

Yusef¹,

Casey²

2019

International Journal of Polymeric Materials and Polymeric Biom

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Here we carried out a comparative study on two cost and time effective methods of encapsulating silver nanoparticles (AgNP) in dipalmitoyl-phosphatidyl choline (DPPC)/cholesterol-based liposome to enhance its cytotoxicity and reduce cytotoxic concentrations and evaluated the effect of this on a blood cell line (THP1 monocytes) often involved in uptake of nanoparticles during human exposure. DLS and Zeta potential analyses over a 6-months period showed the extruded Lipo-AgNP (ExLipo-AgNP) exhibited more stable characteristics when compared with the probe-sonicated Lipo-AgNP (PB-Lipo-AgNP). SEM microscopy indicated agglomeration of the PB-Lipo-AgNP which was not observed in Ex-Lipo-AgNP. Ex-Lipo-AgNP also exhibited higher temperaturedependent stability with 35.3% reduction in size from 20 C to 37 C while PB-Lipo-AgNP was less stable exhibiting 55% size reduction over same temperature range. Load release study over 24 h showed a controlled load release from Ex-Lipo-AgNP while the PB-Lipo-AgNP exhibited burst release at pH 4 and 6.5. Interestingly, it was found that Ex-Lipo-AgNP induced significantly higher toxicity on THP1 cell line after 24 h exposure compared with control unexposed cells; uncoated AgNP and PB-Lipo-AgNP exposed cells at the same concentration. Thus, for the first time, we report that liposomal encapsulation of AgNP by extrusion produces a stable nanocapsule with enhanced cytotoxicity, thus preventing overreliance on high AgNP concentration to achieve desired toxicity.

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Section: Discussionmentioning

confidence: 99%

Evaluation of silver nanoparticle encapsulation in DPPC-based liposome by different methods for enhanced cytotoxicity

Yusef¹,

Casey²

2019

International Journal of Polymeric Materials and Polymeric Biom

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“…Regarding ICP analysis of carrot samples packaged in films containing AgNP, no silver traces were found, which are a indicative that, up to the detection limit of the technique employed (10 ppb), no migration of AgNP occurred. The non-migration of AgNP is highly desirable for food packaging application, avoiding, for instance, toxic effects for humans and animals (Becaro et al 2015b;Griffitt et al 2012). However, the non-migration observed could also be caused by some factors which include relative short contact time between the carrots and the films and the detection limit of equipment (10 ppb) (Simon et al 2008, Walczak et al 2012.…”

Section: Migration Testmentioning

confidence: 99%

Postharvest Quality of Fresh-Cut Carrots Packaged in Plastic Films Containing Silver Nanoparticles

Becaro

Puti

Panosso³

et al. 2015

Food Bioprocess Technol

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Active food packaging containing antimicrobial additive goes beyond traditional functions of packaging, once it can extend food shelf life maintaining its quality, safety and reducing postharvest losses by controlling food spoilage. Among several antimicrobial additives employed in polymeric films for packaging, metallic nanoparticles outstand due to its facility for synthesis, low-cost of production, and intense antimicrobial properties. In this work, extruded plain films of low-density polyethylene (LDPE) containing silver nanoparticles (AgNPs) embedded in SiO 2 and TiO 2 carriers (namely MS and MT, respectively) were produced and used as active packaging for maintaining the physicochemical and microbiological quality of carrots (Daucus Carota L. cv. Brasília). The neat (LDPE) and composite films containing MS and MT were characterized by scanning electron microscopy and permeability to oxygen and used for packaging fresh-cut sliced carrots stored at 10°C for 10 days. After the storage time, the physicochemical properties of carrots were characterized, while the antimicrobial properties of films and AgNP migration were investigated. Our results revealed that both MT and MS packages showed antimicrobial activity even for films containing low concentration of AgNP. In addition, AgNP antimicrobial activity demonstrated to be carrierdependent, once MT-LDPE showed improved performance compared to MS-LDPE. Regarding the physicochemical properties of packaged carrot, lower soluble solids and weight loss and higher levels of ascorbic acid were observed for carrots packaged with MT-LDPE films (compared to MS-LDPE), leading to a better postharvest quality conservation. Such differences observed in physicochemical properties of carrots are related to the distinct antimicrobial and film permeability properties for each composite film. In addition, under the conditions employed in this study, AgNP migration from the packages to fresh-cut carrot was not observed, which is highly desirable for food packaging safety, indicating the potential of such active packages for food preservation application.

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“…An important concern in their use is their stabilization and, thus, the stabilizer used, which includes surfactants and polymers. Poly(vinyl alcohol), for example, is used not only as a stabilizer but also for nucleation, shape-control and enhanced performance [7][8][9][10][11]. The stability of the NP is of concern because instability affects both optical and antibacterial performances [11,12].…”

Section: Introductionmentioning

confidence: 99%

Destabilization of PVA-stabilized Ag NPs: color changes at low aqueous concentrations, induced by aggregation and coalescence

Yang²,

Zhu

et al. 2020

Mater. Res. Express

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Aqueous concentrations of poly(vinyl alcohol) (PVA)-stabilized ∼10 nm silver nanoparticles (Ag NPs), in the 1000 ppm concentration range, have been shown to be highly stable at elevated temperatures. However, lower concentrations of these NPs undergo color changes, without precipitation, when heated or when held for extended periods of time at room temperature. We have studied their optical and morphological changes at 80°C, using UV-vis spectra and TEM, and found that their color, at a concentration of 10 ppm, changes from yellow to claret-red to black without precipitation. Further, the plasmon resonance peak at ∼400 nm diminishes as a new peak develops at ∼550 nm. These changes occur as the previously well-dispersed NPs (yellow color) agglomerate to chains (claret-red color) and, finally, coalesce (black color). We discuss the cause of the instability.

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Toxicity of PVA-stabilized silver nanoparticles to algae and microcrustaceans

Cited by 54 publications

References 44 publications

Evaluation of silver nanoparticle encapsulation in DPPC-based liposome by different methods for enhanced cytotoxicity

Evaluation of silver nanoparticle encapsulation in DPPC-based liposome by different methods for enhanced cytotoxicity

Postharvest Quality of Fresh-Cut Carrots Packaged in Plastic Films Containing Silver Nanoparticles

Destabilization of PVA-stabilized Ag NPs: color changes at low aqueous concentrations, induced by aggregation and coalescence

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