Toxicity of polystyrene nanoplastics in Ctenopharyngodon idella juveniles: A genotoxic, mutagenic and cytotoxic perspective

Guimarães, Abraão Tiago Batista; Estrela, Fernanda Neves; Pereira, Paulo Sérgio; Vieira, Julya Emmanuela de Andrade; Rodrigues, Aline Sueli de Lima; Silva, Fabiano Guimarães; Malafaia, Guilherme

doi:10.1016/j.scitotenv.2020.141937

Cited by 69 publications

(20 citation statements)

References 82 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Since data about the environmental concentrations of PS-MPs in freshwater aquatic ecosystems is limited [42], our experiments were designed mainly to assess the response of both fish species under sublethal PS-MPs concentrations, simulating a highly pessimistic pollution scenario. Our approach was to provoke accelerated effects on fish exposed to higher concentrations than those reported in the field.…”

Section: Ec 50 Estimation (1st Experimental Design)mentioning

confidence: 99%

“…Micro and nano plastic particles can be transferred to living cells through the lymphatic or circulatory system. This results to MPs' dispersion in the whole body and the induction of severe effects such as decreased feeding activity [34], impeded growth and development [35][36][37], endocrine disruption [38] and energy destruction [39], oxidative stress (for a review see [40], immunity and neurotransmission disorders [40,41], genotoxicity [42] and even mortality [37]. Internalization of MPs can also occur after they are adhered to fish skin or other tissues, such as gills [26].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Toxicity and Functional Tissue Responses of Two Freshwater Fish after Exposure to Polystyrene Microplastics

Kaloyianni

Bobori

Xanthopoulou

et al. 2021

Toxics

View full text Add to dashboard Cite

Microplastics (MPs)’ ingestion has been demonstrated in several aquatic organisms. This process may facilitate the hydrophobic waterborne pollutants or chemical additives transfer to biota. In the present study the suitability of a battery of biomarkers on oxidative stress, physiology, tissue function and metabolic profile was investigated for the early detection of adverse effects of 21-day exposure to polystyrene microplastics (PS-MPs, sized 5–12 μm) in the liver and gills of zebrafish Danio rerio and perch, Perca fluviatilis, both of which are freshwater fish species. An optical volume map representation of the zebrafish gill by Raman spectroscopy depicted 5 μm diameter PS-MP dispersed in the gill tissue. Concentrations of PS-MPs close to the EC50 of each fish affected fish physiology in all tissues studied. Increased levels of biomarkers of oxidative damage in exposed fish in relation to controls were observed, as well as activation of apoptosis and autophagy processes. Malondialdehyde (MDA), protein carbonyls and DNA damage responses differed with regard to the sensitivity of each tissue of each fish. In the toxicity cascade gills seemed to be more liable to respond to PS-MPs than liver for the majority of the parameters measured. DNA damage was the most susceptible biomarker exhibiting greater response in the liver of both species. The interaction between MPs and cellular components provoked metabolic alterations in the tissues studied, affecting mainly amino acids, nitrogen and energy metabolism. Toxicity was species and tissue specific, with specific biomarkers responding differently in gills and in liver. The fish species that seemed to be more susceptible to MPs at the conditions studied, was P. fluviatilis compared to D. rerio. The current findings add to a holistic approach for the identification of small sized PS-MPs’ biological effects in fish, thus aiming to provide evidence regarding PS-MPs’ environmental impact on wild fish populations and food safety and adequacy.

show abstract

Section: Ec 50 Estimation (1st Experimental Design)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Toxicity and Functional Tissue Responses of Two Freshwater Fish after Exposure to Polystyrene Microplastics

Kaloyianni

Bobori

Xanthopoulou

et al. 2021

Toxics

View full text Add to dashboard Cite

show abstract

“…Finally, the slides were stained with propidium iodide (PI) and analyzed with a fluorescence microscope (Olympus BX51) under 20 × magnification. One hundred cells were randomly analyzed per blood sample for each fish, and the CaspLab software program was used to measure the percentage of DNA damage in the tail (%DNA damage), Olive tail moment (OTM) and tail length (TL), as described by Guimarães et al [34] . Moreover, the percentage of DNA damage in the tail (%) was classified into five levels according to Trigueiro et al [42] : minimal damage (0–10%), low damage (10–25%), mid damage (25–50%), high damage (50–75%) and extreme damage (75–100%).…”

Section: Methodsmentioning

confidence: 99%

“…The erythrocyte morphometry was performed according to Ahmed and Sheikh [47] with modification. One hundred erythrocyte measurements were randomly processed using ImageJ 1.52av software [34] per fish under a light microscope at a magnification of 100 × magnification (Olympus BX51). The morphometric indices of erythrocytes in the fish were examined using indices as described by Mofizur Rahman and Baek [33] , such as the erythrocyte major axis (EL), erythrocyte minor axis (EW), nucleus major axis (NL) and nucleus minor axis (NW), as shown in Fig.…”

Section: Methodsmentioning

confidence: 99%

“…Importantly, the comet assay and MN and ENA assays can be used to assess environmental toxicity [31] . This information may not be sufficient to determine environmental toxicity, so we decided to use erythrocyte morphometry and erythrocyte maturity determination to assess the cytotoxicity of pollution-sensing organisms that are sensitive enough to detect environmental pollution [32] , [33] , [34] . Several studies have suggested that these techniques are considered effective for the assessment of genotoxicity, mutagenicity and cytotoxicity, particularly in aquatic organisms [35] , [36] .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Investigation of genotoxicity, mutagenicity, and cytotoxicity in erythrocytes of Nile tilapia (Oreochromis niloticus) after fluoxetine exposure

et al. 2022

View full text Add to dashboard Cite

Genotoxic and neurotoxic potential of intracellular nanoplastics: A review

Casella,

Ballaz

2024

J of Applied Toxicology

View full text Add to dashboard Cite

Plastic waste comprises polymers of different chemicals that disintegrate into nanoplastic particles (NPLs) of 1–100‐nm size, thereby littering the environment and posing a threat to wildlife and human health. Research on NPL contamination has up to now focused on the ecotoxicology effects of the pollution rather than the health risks. This review aimed to speculate about the possible properties of carcinogenic and neurotoxic NPL as pollutants. Given their low‐dimensional size and high surface size ratio, NPLs can easily penetrate biological membranes to cause functional and structural damage in cells. Once inside the cell, NPLs can interrupt the autophagy flux of cellular debris, alter proteostasis, provoke mitochondrial dysfunctions, and induce endoplasmic reticulum stress. Harmful metabolic and biological processes induced by NPLs include oxidative stress (OS), ROS generation, and pro‐inflammatory reactions. Depending on the cell cycle status, NPLs may direct DNA damage, tumorigenesis, and lately carcinogenesis in tissues with high self‐renewal capabilities like epithelia. In cells able to live the longest like neurons, NPLs could trigger neurodegeneration by promoting toxic proteinaceous aggregates, OS, and chronic inflammation. NPL genotoxicity and neurotoxicity are discussed based on the gathered evidence, when available, within the context of the intracellular uptake of these newcomer nanoparticles. In summary, this review explains how the risk evaluation of NPL pollution for human health may benefit from accurately monitoring NPL toxicokinetics and toxicodynamics at the intracellular resolution level.

show abstract

Toxicity of polystyrene nanoplastics in Ctenopharyngodon idella juveniles: A genotoxic, mutagenic and cytotoxic perspective

Cited by 69 publications

References 82 publications

Toxicity and Functional Tissue Responses of Two Freshwater Fish after Exposure to Polystyrene Microplastics

Toxicity and Functional Tissue Responses of Two Freshwater Fish after Exposure to Polystyrene Microplastics

Investigation of genotoxicity, mutagenicity, and cytotoxicity in erythrocytes of Nile tilapia (Oreochromis niloticus) after fluoxetine exposure

Genotoxic and neurotoxic potential of intracellular nanoplastics: A review

Contact Info

Product

Resources

About