Toxicity of peritoneal dialysis fluids on cultured fibroblasts, L-929

Abstract

Peritoneal dialysis (PD) fluids are known to suppress the reactions of inflammatory cells in vitro. PD-fluids have also been shown to have cytotoxic influence on mesothelial cells. The combinations of these factors may have a detrimental effect on the peritoneum or may impair cellular defence against bacterial peritonitis. Some authors have discussed the relevance of heat sterilization to both so-called peritoneal side-effects and to chemical decomposition of fluids. Four commercial PD-fluids and one laboratory-made PD-fluid were tested for cytotoxicity on a cultured fibroblast cell line, L-929. Cytotoxicity was determined as an inhibition of cell growth by quantification of total protein. The laboratory-made PD-fluid was sterilized either by filtration or by filtration and heat. The commercial and the heat-sterilized laboratory made PD-fluids caused significant inhibition of cell growth (53 to 76%) in contrast to saline and the filter-sterilized laboratory-made PD-fluid. Since the pH values of all the testsolutions were neutral, low pH was not the cause of toxicity. Our results regarding the L-929 cells indicate that the cytotoxicity of PD-fluids is of a general nature. Furthermore, the results indicate that the heat sterilization process might be partially responsible for causing toxicity in PD-fluids.

Volume Control in Peritoneal Dialysis Patients: Role of New Dialysis Solutions

“…Due to heat sterilization, GDPs are present in standard, glucose-based dialysis fluids, a finding initially demonstrated by Wieslander et al [57,58]. Different in vitro studies with either L929 fibroblasts or isolated human peritoneal mesothelial cells were able to convincingly demonstrate that GDPs impair cellular growth, reduce cell viability and significantly decrease the cellular release from cells of IL-6 and other cyto-or chemokines into the incubation medium [44][45][46][59][60][61].…”

Angiogenic Activity of the Peritoneal Mesothelium: Implications for Peritoneal Dialysis

“…Early experiments pointed to low pH, high concentrations of lactate and glucose, and the presence of glucose degradation products (GDPs) as the elements curtailing biocompatibility of standard PD solutions (Wieslander et al, 1991). In recent years particular attention has been paid to GDPs.…”

Proteomics in Peritoneal Dialysis

“…[Adapted from (Sritippayawan et al 2007)] mitogenic and cytotoxic. Thus, peritoneal dialysis effluent is considered as a significant stressor for the MC layer (Breborowicz et al 1995;Wieslander et al 1991). To address this issue, a cell line derived from the MC layers was used as a model to study the glucoserelated pathways induced by high concentration of glucose (Lechner et al 2010).…”