Toxicity of Metals and Pesticides Using the Sperm Cell Bioassay with the Sea Urchin Arbacia spatuligera

Larrain, Alberto; Riveros, A.; Silva, J.; Bay-Schmith, E.

doi:10.1007/s001289900936

Cited by 17 publications

(13 citation statements)

References 11 publications

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“…The sea urchin fertilization assays were standardized for A. punctulata by the U.S. EPA 600/4-91/003 (1988) and adapted for Arbacia spatuligera by Zuñiga (1999). The assay has been used successfully by Larraín et al (1999), Arévalo et al (2001), and Rudolph et al (2007). Advances in the understanding of sea urchin biology, feeding regimen, and acclimatization to laboratory conditions have been achieved by Silva et al (2004).…”

Section: Introductionmentioning

confidence: 96%

Ecotoxicological sediment evaluations in marine aquaculture areas of Chile

Rudolph

Medina

Urrutia

et al. 2008

Environ Monit Assess

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Given its geographic characteristics, the southern Chilean fjord area is subjected to growing environmental pressure from the development of diverse forms of aquaculture (i.e., fish, algae, shellfish). The sediments accumulate substances as a natural sink, and ecotoxicology assays offer a reliable and robust proxy for sediment quality analyses. This study's objective was to establish a mid-range toxicity base line for the sediments in the region by applying a battery of non-specific ecotoxicological assays. Sediment samples (28) were collected in the channels and fjords studied during the CIMAR-Fiordos 11 cruise (July 2005). The sediments were evaluated using different species endemic to the eastern Pacific as targets: Ampelisca araucana, Tisbe longicornis, Arbacia spatuligera, and Dunaliella tertiolecta. The conditions for each assay were reported previously. Of the four species used as ecotoxicological tools, only D. tertiolecta differed significantly from the control group (negative) in terms of its growth. This difference could be attributed to nutrient enrichment. In general, we concluded that, although local changes occurred in the sediments, the mesoscale magnitude of the ecotoxicological alterations was small. Nonetheless, a surveillance program should be implemented that would allow us to follow-up and analyze the changes that are taking place in the systems on broader scales of time and space.

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Section: Introductionmentioning

confidence: 96%

Ecotoxicological sediment evaluations in marine aquaculture areas of Chile

Rudolph

Medina

Urrutia

et al. 2008

Environ Monit Assess

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“…Copper (Cu) and cadmium (Cd) are among the most dangerous metals for bivalve, 1,2 shrimp 3 and sea urchin larvae. 4 Although Cu is an essential micronutrient for aquatic animals, it can be toxic to marine organisms at elevated concentrations. Background Cu levels in surface water are usually low (0.03-0.23 µg.L -1 ), but can exceed 100 µg.L -1 in severely polluted coastal areas.…”

Section: Introductionmentioning

confidence: 99%

Toxic effects of copper and cadmium on fertilization potency of gametes of Pacific oyster (Crassostrea gigas)

Mai¹,

Morin²,

Cachot³

2013

J Xenobiotics

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“…However, the availability of cell lines has confined the wide utilization of this in vitro assay. Another in vitro acute toxicity assay, sperm cell bioassay, initiated by Dinnel et al (1981), has increasingly become a standard bioassay for evaluation of aquatic pollutants (Larrain et al 1999;McAllister and Kime 2003). In Dinnel's sperm bioassay, the sea urchin sperm were exposed to dilutions of a toxicant for 5 to 15 min before eggs were added for fertilization, which is used as an indicator of sperm viability.…”

Section: Introductionmentioning

confidence: 99%

Exposure of Rosy Barb (Puntius conchonius) Sperm to Abamectin as an In Vitro Assay of Cytotoxicity

Zhang

et al. 2005

Toxicology Mechanisms and Methods

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The acute cytotoxicity of abamectin to the sperm of rosy barb (Puntius conchonius) was tested. It was found that 79% sperm remained motile at 30 min after activation by hypo-osmotic sodium buffer (HSB), while the motility of sperm exposed to 0.14, 0.28, and 0.42 mu M abamectin for 30 min was reduced to 60%, 35%, and 15%, respectively. Exposure to abamectin at 0.56 mu M decreased the motility of sperm to an even lower value of 5.67% after 20 min of exposure. This indicates that the toxicity of abamectin to rosy barb sperm is both dose dependent and exposure time dependent. The ultrastructural examination showed that most of the sperm exposed to 0.14 mu M abamectin for 30 min remained morphologically normal, while the midpiece and its surrounding mitochondria of the sperm treated with 0.28 mu M abamectin for 30 min were severely damaged. For the sperm treated with 0.42 mu M abamectin for 30 min, the majority of the sperm were abnormal. Sperm heads were dissolved and the midpiece broken. The mitochondria are located in the midpiece of the sperm and may be the target of abamectin toxicity in rosy barb sperm. The results also suggest that rosy barb sperm are a suitable candidate for rapid screening of the acute toxicities of bioorganic pesticides.

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Toxicity of Metals and Pesticides Using the Sperm Cell Bioassay with the Sea Urchin Arbacia spatuligera

Cited by 17 publications

References 11 publications

Ecotoxicological sediment evaluations in marine aquaculture areas of Chile

Ecotoxicological sediment evaluations in marine aquaculture areas of Chile

Toxic effects of copper and cadmium on fertilization potency of gametes of Pacific oyster (Crassostrea gigas)

Exposure of Rosy Barb (Puntius conchonius) Sperm to Abamectin as an In Vitro Assay of Cytotoxicity

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