Toxicity of Haemophilus pleuropneumoniae to porcine lung macrophages

Leengoed, L A van; Kamp, E. M.; Pol, J.M.A.

doi:10.1016/0378-1135(89)90099-0

Cited by 28 publications

(21 citation statements)

References 12 publications

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“…Isolation of porcine alveolar and murine peritoneal macrophages Alveolar macrophages were prepared by a modification of the method described by van Leengoed et al (1989). Briefly, adult pig lungs were removed and perfused with sterile phosphate-buffered saline (PBS).…”

Section: Experimental Infection Of Micementioning

confidence: 99%

Bacterial copper‐ and zinc‐cofactored superoxide dismutase contributes to the pathogenesis of systemic salmonellosis

Farrant¹,

Sansone²,

Canvin

et al. 1997

Molecular Microbiology

130

132

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SummaryCopper/zinc-cofactored superoxide dismutase ([Cu,Zn]-SOD) has been found in the periplasm of many bacterial species but its biological function is unknown. Here we report the cloning and characterization of sodC, encoding [Cu,Zn]-SOD, from Salmonella typhimurium. The predicted protein sequence shows only 58% identity to Escherichia coli SodC, and from this its chromosomal location and its immediate proximity to a phage gene, sodC, in Salmonella is speculated to have been acquired by bacteriophagemediated horizontal transfer from an unknown donor. A sodC mutant of S. typhimurium was unimpaired on aerobic growth in rich medium but showed enhanced sensitivity in vitro to the microbicidal action of superoxide. S. typhimurium, S. choleraesuis and S. dublin sodC mutants showed reduced lethality in a mouse model of oral infection and persisted in significantly lower numbers in livers and spleens after intraperitoneal infection, suggesting that [Cu,Zn]-SOD plays a role in pathogenicity, protecting Salmonella against oxygen radical-mediated host defences. There was, however, no observable difference compared with wild type in the interaction of sodC mutants with porcine pleural, mouse peritoneal or J774 macrophages in vitro, perhaps reflecting the hierarchical capacity of different macrophage lines to kill Salmonella, the most efficient overwhelming the proposed protective effect of periplasmic SOD.

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Section: Experimental Infection Of Micementioning

confidence: 99%

Bacterial copper‐ and zinc‐cofactored superoxide dismutase contributes to the pathogenesis of systemic salmonellosis

Farrant¹,

Sansone²,

Canvin

et al. 1997

Molecular Microbiology

130

132

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“…0001-6470 0 1991 SGM been reported (Kume et al, 1986;Rosendal et al, 1988;Van Leengoed et al, 1989).…”

Section: Introductionmentioning

confidence: 99%

The cytotoxin of Actinobacillus pleuropneumoniae (pleurotoxin) is distinct from the haemolysin and is associated with a 120 kDa polypeptide

Rycroft

Williams

Cullen

et al. 1991

Journal of General Microbiology

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Mutants of Actinobacilluspleuropneumoniue strain HK 361 (serotype 2) were isolated which were deficient in type I1 (Ca2+-dependent) haemolysin activity (Hly-). Some of the Hly-mutants secreted a potent, heat-labile extracellular cytotoxic activity against porcine alveolar macrophages. Comparison of cell-free culture supernatant from the parent strain and some Hly-mutants by SDS-PAGE and immunoblotting revealed the loss of a major extracellular polypeptide of 109 kDa. Two Hly-mutants which in addition failed to secrete a 120 kDa polypeptide produced no extracellular cytotoxic activity, suggesting that the 120 kDa protein was the cytotoxin. Antiserum raised to the culture supernatant from a Hly-mutant lacking the 109 kDa polypeptide recognized the 120 kDa band, but not the 109 kDa band, in immunoblots and neutralized the cytotoxic activity, but not the haemolytic activity, of A. pleuropneumoniue. The 120 kDa polypeptide and extracellular cytotoxic activity were widespread among A. pleuropneumoniue strains, but absent from related bacterial pathogens of the pig: Actinobacillus suis, Huemophilus purusuis and Pusteurellu multocidu. A clear correlation was found between the presence of the 120 kDa pdypeptide'and cytotoxic activity in culture supernatants. The cytotoxic activity of all the strains tested was neutralized by antibody to the Hly-extracellular material and by convalescent pig serum. It is proposed that the 120 kDa polypeptide represents the cytotoxin of A. pleuropneumonia?, that it is distinct from the haemolysin, and that it be termed pleurotoxin.

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“…Lung macrophages were collected from piglets aged 8 to 10 weeks (Leengoed et al, 1989) reared conventionally in the premises of the Institute. Tested strains were inoculated into a liquid medium (BHI) supplemented with NAD at 10 µg/ml and incubated overnight at 37°C.…”

Section: Cytotoxicity Testmentioning

confidence: 99%

Prevalence of serotypes, production of Apx toxins, and antibiotic resistance in strains of Actinobacillus pleuropneumoniae isolated in the Czech Republic

Satran¹,

Nedbalcová²

2002

Vet. Med.

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ABSTRACT:Results of serotyping of 122 strains of Actinobacillus pleuropneumoniae isolated on 21 swine farms in the Czech Republic from January 1999 to December 2000 are presented. The highest prevalence was found for serotype 9 (n = 81, 66.4%), followed by serotype 2 (n = 19, 15.7%). Serotypes 1, 3, 5, 7, 8, 11, and 12 were identified only sporadically. Twenty-six of the isolates were tested for gene-toxic profiles and haemolytic and cytotoxic activities. The investigations confirmed the association between serotype on the one hand and Apx genotype and Apx phenotype on the other hand. Results of tests of resistance to antibiotic by the disc diffusion method have justified the apprehension of development of resistance due to long-term treatment with antibiotics. Strains with full or intermediary resistance against all the commonly used penicillin-and tetracycline-type antibiotics were found. None of the strains was resistant to florfenicol, norfloxacin, or cefalotine.

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Toxicity of Haemophilus pleuropneumoniae to porcine lung macrophages

Cited by 28 publications

References 12 publications

Bacterial copper‐ and zinc‐cofactored superoxide dismutase contributes to the pathogenesis of systemic salmonellosis

Bacterial copper‐ and zinc‐cofactored superoxide dismutase contributes to the pathogenesis of systemic salmonellosis

The cytotoxin of Actinobacillus pleuropneumoniae (pleurotoxin) is distinct from the haemolysin and is associated with a 120 kDa polypeptide

Prevalence of serotypes, production of Apx toxins, and antibiotic resistance in strains of Actinobacillus pleuropneumoniae isolated in the Czech Republic

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