Toxicity of graphene oxide and multi-walled carbon nanotubes against human cells and zebrafish

Chen, Liqiang; Hu, Pingping; Li, Zhang; Huang, Sizhou; Luo, Lei; Huang, Chengzhi

doi:10.1007/s11426-012-4620-z

Cited by 147 publications

(71 citation statements)

References 38 publications

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“…[30][31][32]62,67 Compared with the blank control, neither sublethal toxicity nor drop of hatching rate was detectable in the presence of lGO/NPcurcumin complexes. Screening of phenotypic toxicity may be implemented because of opaque quality of tissues following cell death.…”

Section: ■ Results and Discussionmentioning

confidence: 89%

“…Screening of phenotypic toxicity may be implemented because of opaque quality of tissues following cell death. 62 In the enlarged view of zebrafish group after treatment with lGO/NP/curcumin complexes, we did not notice any obvious abnormality of the adult zebrafish regarding the blood vessel, muscle and the internal organs ( Figure S3). 63 It is suggested that the zebrafish could grow healthily in the presence of lGO/NP/curcumin complexes.…”

Section: ■ Results and Discussionmentioning

confidence: 91%

“…33,61 Furthermore, biology and optical clarity of zebrafish allow the visual screen of the whole organism at all stages of embryonic development. 33,62 Noteworthy, a short-term zebrafish biotoxicity assessment could be more or less linked to the developmental biotoxicity during the long-term human growth. 32,63 Herein, we investigated the biotoxicity of graphene-based nanomaterials using zebrafish as a vertebrate model from the embryo through the hatchling stage.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

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Graphene-Based Anticancer Nanosystem and Its Biosafety Evaluation Using a Zebrafish Model

et al. 2013

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In this paper, a facile strategy to develop graphenebased delivery nanosystems for effective drug loading and sustained drug release was proposed and validated. Specifically, biocompatible naphthalene-terminated PEG (NP) and anticancer drugs (curcumin or doxorubicin (DOX)) were simultaneously integrated onto oxidized graphene (GO), leading to selfassembled, nanosized complexes. It was found that the oxidation degree of GO had a significant impact on the drug-loading efficiency and the structural stability of nanosystems. Interestingly, the nanoassemblies resulted in more effective cellular entry of DOX in comparison with free DOX or DOX-loaded PEGpolyester micelles at equivalent DOX dose, as demonstrated by confocal microscopy studies. Moreover, the nanoassemblies not only exhibited a sustained drug release pattern without an initial burst release, but also significantly improved the stability of formulations which were resistant to drug leaking even in the presence of strong surfactants such as aromatic sodium benzenesulfonate (SBen) and aliphatic sodium dodecylsulfonate (SDS). In addition, the nanoassemblies without DOX loading showed negligible in vitro cytotoxicity, whereas DOX-loaded counterparts led to considerable toxicity against HeLa cells. The DOX-mediated cytotoxicity of the graphene-based formulation was around 20 folds lower than that of free DOX, most likely due to the slow DOX release from complexes. A zebrafish model was established to assess the in vivo safety profile of curcumin-loaded nanosystems. The results showed they were able to excrete from the zebrafish body rapidly and had nearly no influence on the zebrafish upgrowth. Those encouraging results may prompt the advance of graphene-based nanotherapeutics for biomedical applications.

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Section: ■ Results and Discussionmentioning

confidence: 89%

Section: ■ Results and Discussionmentioning

confidence: 91%

Section: ■ Results and Discussionmentioning

confidence: 99%

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Graphene-Based Anticancer Nanosystem and Its Biosafety Evaluation Using a Zebrafish Model

et al. 2013

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“…There have been several reports in the literature demonstrating the toxicity of different CNPs in various biological embryo models, such as chicken, [16][17][18][19] zebrafish, [20][21][22][23][24][25] and Xenopus laevis. 26,27 For example, carbon nanotubes inhibited angiogenesis of the chorioallantoic membrane in chicken embryos and caused death before day 12 of the incubation period.…”

Section: Introductionmentioning

confidence: 99%

“…21 In the case of GO, dose-dependent gross morphological defects, hatching delays, and death of zebrafish embryos were observed. 20,23,24 In in vivo experiments with X. laevis embryos, ND was highly embryotoxic and teratogenic. 27 Nevertheless, the investigations described only focused on particular nanomaterials without comparing the biological effects of different carbon allotropes manufactured by different methods and with diverse physicochemical properties.…”

Section: Introductionmentioning

confidence: 99%

Toxicity studies of six types of carbon nanoparticles in a chicken-embryo model

Kurantowicz

Sawosz

Halik

et al. 2017

IJN

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In the present study, the toxicity of six different types of carbon nanoparticles (CNPs) was investigated using a chicken-embryo model. Fertilized chicken eggs were divided into the following treatment groups: placebo, diamond NPs, graphite NPs, pristine graphene, small graphene oxide, large graphene oxide, and reduced graphene oxide. Experimental solutions at a concentration of 500 μg/mL were administrated into the egg albumin. Gross pathology and the rate of survival were examined after 5, 10, 15, and 20 days of incubation. After 20 days of incubation, blood samples were collected and the weight of the body and organs measured. The relative ratio of embryo survival decreased after treatment all treatments except diamond NPs. There was no correlation between the rate of survival and the ζ-potential or the surface charge of the CNPs in solution. Body and organ weight, red blood-cell morphology, blood serum biochemical parameters, and oxidative damage in the liver did not differ among the groups. These results indicate that CNPs can remain in blood circulation without any major side effects, suggesting their potential applicability as vehicles for drug delivery or active compounds per se. However, there is a need for further investigation of their properties, which vary depending on production methods and surface functionalization.

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Black Phosphorus: Degradation Mechanism, Passivation Method, and Application for In Situ Tissue Regeneration

Xie

Abbasi

Xiao

et al. 2020

Adv Materials Inter

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have been already applied for the restoration. [2] During the development of the treatments, the term "tissue engineering" was advanced in the 1980s, in which the biological and engineering principles are combined to fabricate functional replacements for regenerating injured tissues. [3] However, the classical tissue engineering strategy needs a lot of time and much laborious effort for extensive cell expansion steps. In the past few decades, a new idea, known as in situ tissue regeneration (ISTR), was introduced. ISTR means that the host endogenous stem cells or tissuespecific progenitor cells are guided to the location of trauma for tissue recovery in vivo by implanting specific biomaterials system without complicated in vitro manipulation. [4] Many biomaterials, including natural biomaterials, [5] synthetic biopolymers, [6] and bioceramics, [7] have been widely applied for ISTR. The degradation rate of materials is crucial to the successful ISTR, as a rapid degradation may influence the properties of implanted biomaterial composites, for example, causing a mechanical failure. From the other side, if the composite degrades too sluggishly, an inflammation might occur and impair the recovery of damaged tissue. [8] Along with the rapid development of nanomaterials, the 2D materials [9] have attracted ever-increasing interest in tissue regeneration, owing to their remarkable optical properties, biocompatibility, large specific surface area, and high payload of drugs and agents. However, the ISTR application area is limited by the poor biodegradability of conventional 2D nanomaterials. Attributed to the excellent biodegradation ability, 2D black phosphorus (BP) has stepped into people's horizon, which shows more promising potentials in organism restoration over other 2D materials. The bulk form of BP was first synthesized by Bridgman in 1914 from white phosphorus with a high-pressure synthesis method. [10] Nevertheless, the BP crystal did not gain much attention due to the small band gap and the difficulty of controlling the material quality, [11] until a monolayer (ML) of BP crystal was introduced by several research groups in 2014. [12] The 2D nanomaterial of BP, also known as phosphorene, has several different properties from other 2D materials, e.g., graphene. One different major point is that the lone pair electron makes phosphorus atom be an active chemical adsorption position for oxygen molecules. With the rapid increase of BP surface, phosphorene will chemically decompose very quickly Nanomaterials have attracted ever-increasing interest in tissue regeneration in the past few decades. As an emerging 2D layered nanomaterial, black phosphorus shows more promising potentials in organism restoration than other 2D materials, attributed to its extraordinary biodegradation ability, remarkable optical properties, and high payload of drugs and agents. The degradation products, i.e., phosphate, can be adsorbed by tissues for specific regeneration such as bone, yet the excessive degradation rate of black phosphorus wil...

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Toxicity of graphene oxide and multi-walled carbon nanotubes against human cells and zebrafish

Cited by 147 publications

References 38 publications

Graphene-Based Anticancer Nanosystem and Its Biosafety Evaluation Using a Zebrafish Model

Graphene-Based Anticancer Nanosystem and Its Biosafety Evaluation Using a Zebrafish Model

Toxicity studies of six types of carbon nanoparticles in a chicken-embryo model

Black Phosphorus: Degradation Mechanism, Passivation Method, and Application for In Situ Tissue Regeneration

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