Toxicity of Fumonisins, Deoxynivalenol, and Zearalenone Alone and in Combination in Turkeys Fed with the Maximum European Union–Tolerated Level

Travel, Angélique; Métayer, J. P.; Mika, A.; Bailly, Jean‐Denis; Cleva, Didier; Boissieu, Cyril; Guennec, Jean Le; Albaric, Olivier; Labrut, Sophie; Lepivert, Gurvan; Marengue, Eric; Froment, Pascal; Tardieu, Didier; Guerre, Philippe

doi:10.1637/aviandiseases-d-19-00073

Cited by 16 publications

(26 citation statements)

References 75 publications

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“…This study was done to compare different scenarios of exposure in two avian species to highlight between-protocol convergences and divergences. No effects on health and performance were observed whatever the diet fed [ 27 , 28 ]. Special attention was given to the measurement of α-ZOL and β-ZOL, which strongly vary in their toxicity [ 1 , 47 ].…”

Section: Resultsmentioning

confidence: 99%

“…All experiments with animals were approved on May 18, 2017 by the French Ministry of Higher Education and Research and registered under number 02032.01. Tissue samples were obtained from turkeys and chickens reared in individual cages and fed ad libitum with different diets containing fusariotoxins as previously described [ 27 , 28 ]. Briefly, the diets containing mycotoxins were formulated on a corn/soybean basis by incorporation of ground cultured toxigenic Fusarium strains to reach a ZEN concentration of 0.5 mg/kg and concentrations of ZEN, DON, and FB1+FB2 of 0.5, 5, and 20 mg/kg, respectively.…”

Section: Methodsmentioning

confidence: 99%

“…The control diets were considered to be free of mycotoxins. The final concentrations of ZEN, DON, and FB, and the concentrations of other mycotoxins that could contaminate the diets, were measured by HPLC-MSMS according to AFNOR NFV03-110 as previously described, and did not vary much from the targeted concentrations [ 27 , 28 , 53 ]. Each of the experimental diets was distributed to 14 turkeys from the 55th to the 70th day of age and to 14 broilers from the 1st to the 35th day of age.…”

Section: Methodsmentioning

confidence: 99%

“…Studies were performed in turkeys and broiler chickens to compare the metabolism of ZEN into α-ZOL and β-ZOL, which has been reported to vary across species [ 5 , 26 ]. Exposure was conducted at a low dose that did not demonstrate any signs of toxicity [ 27 , 28 ]. Because deoxynivalenol (DON) and fumonisins (FB) often co-occur in feed with ZEN, different diets were compared in each species, one containing ZEN alone and another containing ZEN, DON, and FB.…”

Section: Introductionmentioning

confidence: 99%

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Zearalenone and Metabolites in Livers of Turkey Poults and Broiler Chickens Fed with Diets Containing Fusariotoxins

Tardieu

Travel

Métayer

et al. 2020

Toxins

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Zearalenone (ZEN) and metabolites were measured in livers of turkeys and broilers fed a control diet free of mycotoxins, a diet that contained 0.5 mg/kg ZEN (ZEN diet), and a diet that contained 0.5, 5, and 20 mg/kg of ZEN, fumonisins, and deoxynivalenol, respectively (ZENDONFB diet). The feed was individually distributed to male Grade Maker turkeys from the 55th to the 70th day of age and to male Ross chickens from the 1st to the 35th day of age, without any signs of toxicity. Together, the free and conjugated forms of ZEN, α- and β-zearalenols (ZOLs), zearalanone (ZAN), and α- and β-zearalanols (ZALs) were measured by UHPLC-MS/MS with [13C18]-ZEN as an internal standard and immunoaffinity clean-up of samples. ZAN and ZALs were not detected. ZEN and ZOLs were mainly found in their conjugated forms. α-ZOL was the most abundant and was found at a mean concentration of 2.23 and 1.56 ng/g in turkeys and chickens, respectively. Consuming the ZENDONFB diet significantly increased the level of total metabolites in the livers of chickens. Furthermore, this increase was more pronounced for the free forms of α-ZOL than for the conjugated forms. An investigation of the presence of ZEN and metabolites in muscle with the methods validated for the liver failed to reveal any traces of these contaminants in this tissue. These results suggest that concomitant dietary exposure to deoxynivalenol (DON) and fumonisins (FB) may alter the metabolism and persistence of ZEN and its metabolites in the liver.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Zearalenone and Metabolites in Livers of Turkey Poults and Broiler Chickens Fed with Diets Containing Fusariotoxins

Tardieu

Travel

Métayer

et al. 2020

Toxins

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“…To this end, we first developed an UPLC-MS/MS method that enables simultaneous quantitation of FB1, FB2, and FB3 in muscle and liver. We then applied the method to analyze samples that had been taken as a part of two toxicological studies in chickens and turkey fed with FB alone, and fed with FB in combination with DON and ZEN [34,35].…”

Section: Introductionmentioning

confidence: 99%

Fumonisin B1, B2 and B3 in Muscle and Liver of Broiler Chickens and Turkey Poults Fed with Diets Containing Fusariotoxins at the EU Maximum Tolerable Level

Tardieu

Travel

Métayer

et al. 2019

Toxins

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Although provisional maximum tolerable daily intake and recommended guidelines have been established for fumonisins (FB) in food, few data are available concerning levels of FB in edible animal tissues. Such data are of particular interest in avian species that can tolerate relatively high levels of fumonisins in their feed. Also, even if multiple contamination of animal feed by toxins produced by Fusarium is very frequent, little is known about the consequences of multiple contamination for FB levels in tissues. The aim of this study was to analyze the concentrations of FB in the muscle and liver of chickens and turkeys fed with FB alone and with FB combined with deoxynivalenol (DON), and with zearalenone (ZEN). Experimental diets were formulated by incorporating ground cultured toxigenic Fusarium strains in corn-soybean based feeds. Control diets were free of mycotoxins, FB diets contained 20 mg FB1+FB2/kg, and FBDONZEN diets contained 20, 5, and 0.5 mg/kg of FB1+FB2, DON, and ZEN, respectively. Animals were reared in individual cages with free access to water and feed. The feed was distributed to male Ross chickens from the 1st to the 35th day of age and to male Grade Maker turkeys from the 55th to the 70th day of age. On the last day of the study, the birds were starved for eight hours, killed, and autopsied for tissues sampling. No sign of toxicity was observed. A UHPLC-MS/MS method with isotopic dilution and immunoaffinity clean-up of samples has been developed for analysis of FB in muscle (n = 8 per diet) and liver (n = 8 per diet). Only traces of FB that were below the LOQ of 0.25 µg/kg were found in most of the samples of animals fed with the control diets. Mean concentrations of FB1, FB2, and FB3 in muscle were 17.5, 3.39, and 1.26 µg/kg, respectively, in chickens, and 5.77, 1.52, and 0.54 µg/kg in turkeys, respectively. In the liver, the respective FB1, FB2, and FB3 concentrations were 44.7, 2.61, and 0.79 µg/kg in chickens, and 41.47, 4.23, and 1.41 µg/kg, in turkeys. Cumulated level of FB1+FB2+FB3 in the highly contaminated samples were above 60 and 100 µg/kg in muscle and liver, respectively. The concentrations of FB in the tissues of animals fed the FBDONZEN diet did not greatly differ from the concentrations measured in animals fed the diet containing only FB.

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The natural occurrence, toxicity mechanisms and management strategies of Fumonisin B1：A review

Gao

Luo

Zhu

et al. 2023

Environmental Pollution

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Toxicity of Fumonisins, Deoxynivalenol, and Zearalenone Alone and in Combination in Turkeys Fed with the Maximum European Union–Tolerated Level

Cited by 16 publications

References 75 publications

Zearalenone and Metabolites in Livers of Turkey Poults and Broiler Chickens Fed with Diets Containing Fusariotoxins

Zearalenone and Metabolites in Livers of Turkey Poults and Broiler Chickens Fed with Diets Containing Fusariotoxins

Fumonisin B1, B2 and B3 in Muscle and Liver of Broiler Chickens and Turkey Poults Fed with Diets Containing Fusariotoxins at the EU Maximum Tolerable Level

The natural occurrence, toxicity mechanisms and management strategies of Fumonisin B1：A review

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