Toxicity of Four Dibenzoylhydrazine Correlates with Evagination-Induction in the Cotton Leafworm

Smagghe, Guy; Carton, Bert; Heirman, Ans; Tirry, Luc

doi:10.1006/pest.2000.2497

Cited by 29 publications

(25 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…With laboratory susceptibles, we have previously measured a K D value of 6.211 nM (Smagghe et al, 2000), which is not significantly different from the K D value that we obtained for multi-resistant strains in this study. In addition, we confirmed the presence of EcRs in the nuclei of imaginal wing discs with a common monoclonal antibody.…”

Section: Discussioncontrasting

confidence: 45%

“…So, the current assays indicated that imaginal disc evagination-induction is a good approximation of the potency for both ecdysteroids and ecdysone agonists. Comparison of the current data with those using imaginal discs of S. littoralis from a laboratory susceptible colony (Smagghe et al, 2000) and of S. exigua (Smagghe et al, 1999a) revealed a high similarity between Lepidoptera species. This supports the utility of such imaginal disc bioassays for evaluating putative ecdysone agonists.…”

Section: Discussionmentioning

confidence: 67%

“…Data are compared to laboratory susceptible strains (S) of S. littoralis (Smagghe et al, 2000). matic benzoyl rings, should be in favor of the rate of immediate passage and elimination via the gut without uptake in the body hemocoel, particularly in multi-resistant larvae.…”

Section: Discussionmentioning

confidence: 99%

“…4B) revealed an equilibrium binding constant K D of 8.065 nM and a theoretical number of receptors β max of 138 fM per 20 isolated imaginal wing discs. Similarly, K D was 6.211 nM and β max 181 fM for imaginal wing discs from laboratory susceptible strains (Smagghe et al, 2000).…”

Section: Immunolocalisation Of Ecrs and Scatchard Plot Binding Assay mentioning

confidence: 99%

See 3 more Smart Citations

Significance of absorption, oxidation, and binding to toxicity of four ecdysone agonists in multi‐resistant cotton leafworm

Smagghe

Carton

Decombel

et al. 2001

Arch Insect Biochem Physiol

Self Cite

View full text Add to dashboard Cite

Treatment of last-instar larvae of multi-resistant cotton leafworm Spodoptera littoralis with four dibenzoylhydrazines, methoxyfenozide (RH-2485), tebufenozide (RH-5992), halofenozide (RH-0345), and RH-5849, resulted in premature molting leading to death. Methoxyfenozide was the most toxic followed by tebufenozide, halofenozide, and RH-5849. To explain differences in toxicity, especially between multi-resistant and laboratory strains, absorption in the body tissues and oxidative metabolism were tested with 14C-labeled ecdysone agonist and a Lineweaver-Burk assay, respectively. Then to address different compound potencies in multi-resistant strains, the potency of the four ecdysone agonists was measured based on their ability to mimic the natural insect molting hormone, 20-hydroxyecdysone (20E) by inducing evagination in isolated imaginal wing discs. Using monoclonal antibody 9B9, the presence of ecdysteroid receptors in imaginal discs in vitro was confirmed. In parallel, Scatchard plot analysis with whole imaginal wing discs cultured with different concentrations of 3H-labeled ponasterone A indicated no significant difference in affinity and in number of target sites for binding between multi-resistant and susceptible laboratory strains. The four compounds tested caused the effect as agonists of 20E in vitro, and typically the order of their toxicities (LC50s) corresponded with that for evagination-induction with whole imaginal discs.

show abstract

Section: Discussioncontrasting

confidence: 45%

Section: Discussionmentioning

confidence: 67%

Section: Discussionmentioning

confidence: 99%

Section: Immunolocalisation Of Ecrs and Scatchard Plot Binding Assay mentioning

confidence: 99%

See 2 more Smart Citations

Significance of absorption, oxidation, and binding to toxicity of four ecdysone agonists in multi‐resistant cotton leafworm

Smagghe

Carton

Decombel

et al. 2001

Arch Insect Biochem Physiol

Self Cite

View full text Add to dashboard Cite

show abstract

“…Methoxyfenozide has been shown to be more effective than tebufenozide on armyworms, Spodoptera sp and other pests (Smagghe et al, 2000). Methoxyfenozide is an effective agent for control of codling moth Cydia pomonella (Smagghe et al, 1998), Southwestern corn borer, Distraea grandiosella, European corn borer Ostrinia nubilalis, rice stem borers and cotton leaf worm.…”

Section: Methoxyfenozidementioning

confidence: 99%

A New Method Development and Validation for Identification and Quantification of Methoxyfenozide Insecticide Residues in Grapes using High Performance Liquid Chromatography

Murthy¹,

Rao²,

Revathi³

et al. 2017

Int.J.Curr.Microbiol.App.Sci

View full text Add to dashboard Cite

Growth and mitogenic effects of arylphorin in vivo and in vitro

Blackburn

Cortí

et al. 2007

Arch Insect Biochem Physiol

View full text Add to dashboard Cite

In insects, developmental responses are organ- and tissue-specific. In previous studies of insect midgut cells in primary tissue cultures, growth-promoting and differentiation factors were identified from the growth media, hemolymph, and fat body. Recently, it was determined that the mitogenic effect of a Manduca sexta fat body extract on midgut stem cells of Heliothis virescens was due to the presence of monomeric alpha-arylphorin. Here we report that in primary midgut cell cultures, this same arylphorin stimulates stem cell proliferation in the lepidopterans M. sexta and Spodoptera littoralis, and in the beetle Leptinotarsa decemlineata. Studies using S. littoralis cells confirm that the mitogenic effect is due to free alpha-arylphorin subunits. In addition, feeding artificial diets containing arylphorin increased the growth rates of several insect species. When tested against continuous cell lines, including some with midgut and fat body origins, arylphorin had no effect; however, a cell line derived from Lymantria dispar fat body grew more rapidly in medium containing a chymotryptic digest of arylphorin.

show abstract

Toxicity of Four Dibenzoylhydrazine Correlates with Evagination-Induction in the Cotton Leafworm

Cited by 29 publications

References 19 publications

Significance of absorption, oxidation, and binding to toxicity of four ecdysone agonists in multi‐resistant cotton leafworm

Significance of absorption, oxidation, and binding to toxicity of four ecdysone agonists in multi‐resistant cotton leafworm

A New Method Development and Validation for Identification and Quantification of Methoxyfenozide Insecticide Residues in Grapes using High Performance Liquid Chromatography

Growth and mitogenic effects of arylphorin in vivo and in vitro

Contact Info

Product

Resources

About