Toxicity of Dimethyl Sulfoxide as a Solvent in Bioassay System with HeLa Cells Evaluated Colorimetrically with 3-(4,5-Dimethyl thiazol-2-yl)-2,5-diphenyl-tetrazolium Bromide

Abstract

The toxicity of dimethyl sulfoxide (Me2SO) was examined in HeLa cells cultured at 37 degrees C for up to 72 hr. The growth of the cells was measured by a colorimetric method with the use of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), which gave good correlation between the cell number and the color development from the reduction of MTT under suitable conditions. When the initial number of cells was 3 x 10(4)/ml, Me2SO at 1% or less had no apparent effect on proliferation for up to 48 hr of incubation, but in longer incubations, cell growth was repressed. When the initial number of cells was 3 x 10(5)/ml, the effect of Me2SO was similar.

Decomposition and detoxification of aflatoxin B1 by lactic acid

“…These samples were dissolved in dimethyl sulfoxide (DMSO; Rankem, India), which was then diluted with DMEM. The concentration of DMSO in the test samples was kept at 1% …”

The H4IIE Cell Bioassay as an Indicator of Dioxin-like Chemicals in Wildlife and the Environment

“…It has been suggested that the amount of carrier solvent used should not exceed 0.5% of the total solution to eliminate interference or toxicity due to the presence of excess carrier solvent (Bradlaw and Casterline, 1979;Harris et al, 1989). Trivedi et al (1990) examined the toxicity of DMSO to the human carcinoma cell line HeLa. When the initial number of cells plated was 3 × 10 4 /ml, DMSO concentration of 1% or less had no apparent effect on the rate of proliferation for up to 48 h of incubation at 37 • C. In incubations up to 72 h, cell growth was repressed by 1% DMSO.…”

Synthesis, Biological Evaluation and Molecular Docking of Novel Indole-Aminoquinazoline Hybrids for Anticancer Properties

“…The compounds were assayed as dimethyl sulfoxide (DMSO) solutions at the following concentrations: 5, 12.5, 25, 50, 100 μM, against gefitinib as a reference standard. DMSO was used as a negative control of choice since it has been found before to have no apparent effect on proliferation of the HeLa, Caco-2, and C3A cells at 1% or less for up to 48 h [22,23]. Gefitinib used as a positive control, on the other hand, has previously been found to induce autophagy in lung cancer A549 cells and to enhance apoptosis and suppress cancer cell proliferation [2].…”